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New insights into the origin and evolution of α-amylase genes in green plants

Gene duplication is a source of genetic materials and evolutionary changes, and has been associated with gene family expansion. Functional divergence of duplicated genes is strongly directed by natural selections such as organism diversification and novel feature acquisition. We show that, plant α-a...

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Autores principales: Ju, Liangliang, Pan, Zhifen, Zhang, Haili, Li, Qiao, Liang, Junjun, Deng, Guangbing, Yu, Maoqun, Long, Hai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426938/
https://www.ncbi.nlm.nih.gov/pubmed/30894656
http://dx.doi.org/10.1038/s41598-019-41420-w
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author Ju, Liangliang
Pan, Zhifen
Zhang, Haili
Li, Qiao
Liang, Junjun
Deng, Guangbing
Yu, Maoqun
Long, Hai
author_facet Ju, Liangliang
Pan, Zhifen
Zhang, Haili
Li, Qiao
Liang, Junjun
Deng, Guangbing
Yu, Maoqun
Long, Hai
author_sort Ju, Liangliang
collection PubMed
description Gene duplication is a source of genetic materials and evolutionary changes, and has been associated with gene family expansion. Functional divergence of duplicated genes is strongly directed by natural selections such as organism diversification and novel feature acquisition. We show that, plant α-amylase gene family (AMY) is comprised of six subfamilies (AMY1-AMY6) that fell into two ancient phylogenetic lineages (AMY3 and AMY4). Both AMY1 and AMY2 are grass-specific and share a single-copy ancestor, which is derived from grass AMY3 genes that have undergone massive tandem and whole-genome duplications during evolution. Ancestral features of AMY4 and AMY5/AMY6 genes have been retained among four green algal sequences (Chrein_08.g362450, Vocart_0021s0194, Dusali_0430s00012 and Monegl_16464), suggesting a gene duplication event following Chlorophyceae diversification. The observed horizontal gene transfers between plant and bacterial AMYs, and chromosomal locations of AMY3 and AMY4 genes in the most ancestral green body (C. reinhardtii), provide evidences for the monophyletic origin of plant AMYs. Despite subfamily-specific sequence divergence driven by natural selections, the active site and SBS1 are well-conserved across different AMY isoforms. The differentiated electrostatic potentials and hydrogen bands-forming residue polymorphisms, further imply variable digestive abilities for a broad substrates in particular tissues or subcellular localizations.
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spelling pubmed-64269382019-03-28 New insights into the origin and evolution of α-amylase genes in green plants Ju, Liangliang Pan, Zhifen Zhang, Haili Li, Qiao Liang, Junjun Deng, Guangbing Yu, Maoqun Long, Hai Sci Rep Article Gene duplication is a source of genetic materials and evolutionary changes, and has been associated with gene family expansion. Functional divergence of duplicated genes is strongly directed by natural selections such as organism diversification and novel feature acquisition. We show that, plant α-amylase gene family (AMY) is comprised of six subfamilies (AMY1-AMY6) that fell into two ancient phylogenetic lineages (AMY3 and AMY4). Both AMY1 and AMY2 are grass-specific and share a single-copy ancestor, which is derived from grass AMY3 genes that have undergone massive tandem and whole-genome duplications during evolution. Ancestral features of AMY4 and AMY5/AMY6 genes have been retained among four green algal sequences (Chrein_08.g362450, Vocart_0021s0194, Dusali_0430s00012 and Monegl_16464), suggesting a gene duplication event following Chlorophyceae diversification. The observed horizontal gene transfers between plant and bacterial AMYs, and chromosomal locations of AMY3 and AMY4 genes in the most ancestral green body (C. reinhardtii), provide evidences for the monophyletic origin of plant AMYs. Despite subfamily-specific sequence divergence driven by natural selections, the active site and SBS1 are well-conserved across different AMY isoforms. The differentiated electrostatic potentials and hydrogen bands-forming residue polymorphisms, further imply variable digestive abilities for a broad substrates in particular tissues or subcellular localizations. Nature Publishing Group UK 2019-03-20 /pmc/articles/PMC6426938/ /pubmed/30894656 http://dx.doi.org/10.1038/s41598-019-41420-w Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ju, Liangliang
Pan, Zhifen
Zhang, Haili
Li, Qiao
Liang, Junjun
Deng, Guangbing
Yu, Maoqun
Long, Hai
New insights into the origin and evolution of α-amylase genes in green plants
title New insights into the origin and evolution of α-amylase genes in green plants
title_full New insights into the origin and evolution of α-amylase genes in green plants
title_fullStr New insights into the origin and evolution of α-amylase genes in green plants
title_full_unstemmed New insights into the origin and evolution of α-amylase genes in green plants
title_short New insights into the origin and evolution of α-amylase genes in green plants
title_sort new insights into the origin and evolution of α-amylase genes in green plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426938/
https://www.ncbi.nlm.nih.gov/pubmed/30894656
http://dx.doi.org/10.1038/s41598-019-41420-w
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