Identification of new DNA-associated proteins from Waddlia chondrophila

Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to ma...

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Autores principales: de Barsy, Marie, Herrgott, Lucas, Martin, Virginie, Pillonel, Trestan, Viollier, Patrick H., Greub, Gilbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426960/
https://www.ncbi.nlm.nih.gov/pubmed/30894592
http://dx.doi.org/10.1038/s41598-019-40732-1
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author de Barsy, Marie
Herrgott, Lucas
Martin, Virginie
Pillonel, Trestan
Viollier, Patrick H.
Greub, Gilbert
author_facet de Barsy, Marie
Herrgott, Lucas
Martin, Virginie
Pillonel, Trestan
Viollier, Patrick H.
Greub, Gilbert
author_sort de Barsy, Marie
collection PubMed
description Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ(66)), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain.
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spelling pubmed-64269602019-03-28 Identification of new DNA-associated proteins from Waddlia chondrophila de Barsy, Marie Herrgott, Lucas Martin, Virginie Pillonel, Trestan Viollier, Patrick H. Greub, Gilbert Sci Rep Article Transcriptional regulation in Chlamydiae is still poorly understood. The absence until recently of genetic tools is the main cause of this gap. We discovered three new potential DNA-associated proteins of Waddlia chondrophila, a Chlamydia-related bacterium, using heparin chromatography coupled to mass spectrometry (Wcw_0377, Wcw_1456, and Wcw_1460). By ChIP-seq analysis, we determined the regulatory landscape of these three proteins and we showed that Wcw_0377 binds all along the genome whereas Wcw_1456 and _1460 possess a wide regulon with a large number of co-regulated genes. Wcw_1456 and Wcw_1460 interact with RpoD (σ(66)), emerging as potential RpoD regulators. On the other hand, Wcw_0377 is able to reach the host nucleus, where it might interact with eukaryotic histones through its putative chromatin-remodelling SWIB/MDM2 domain. Nature Publishing Group UK 2019-03-20 /pmc/articles/PMC6426960/ /pubmed/30894592 http://dx.doi.org/10.1038/s41598-019-40732-1 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
de Barsy, Marie
Herrgott, Lucas
Martin, Virginie
Pillonel, Trestan
Viollier, Patrick H.
Greub, Gilbert
Identification of new DNA-associated proteins from Waddlia chondrophila
title Identification of new DNA-associated proteins from Waddlia chondrophila
title_full Identification of new DNA-associated proteins from Waddlia chondrophila
title_fullStr Identification of new DNA-associated proteins from Waddlia chondrophila
title_full_unstemmed Identification of new DNA-associated proteins from Waddlia chondrophila
title_short Identification of new DNA-associated proteins from Waddlia chondrophila
title_sort identification of new dna-associated proteins from waddlia chondrophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426960/
https://www.ncbi.nlm.nih.gov/pubmed/30894592
http://dx.doi.org/10.1038/s41598-019-40732-1
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