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Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy

Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effectiv...

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Autores principales: Scholz, Gregor, Mariana, Shinta, Dharmawan, Agus Budi, Syamsu, Iqbal, Hörmann, Philipp, Reuse, Carsten, Hartmann, Jana, Hiller, Karsten, Prades, Joan Daniel, Wasisto, Hutomo Suryo, Waag, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427468/
https://www.ncbi.nlm.nih.gov/pubmed/30862101
http://dx.doi.org/10.3390/s19051234
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author Scholz, Gregor
Mariana, Shinta
Dharmawan, Agus Budi
Syamsu, Iqbal
Hörmann, Philipp
Reuse, Carsten
Hartmann, Jana
Hiller, Karsten
Prades, Joan Daniel
Wasisto, Hutomo Suryo
Waag, Andreas
author_facet Scholz, Gregor
Mariana, Shinta
Dharmawan, Agus Budi
Syamsu, Iqbal
Hörmann, Philipp
Reuse, Carsten
Hartmann, Jana
Hiller, Karsten
Prades, Joan Daniel
Wasisto, Hutomo Suryo
Waag, Andreas
author_sort Scholz, Gregor
collection PubMed
description Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility.
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spelling pubmed-64274682019-04-15 Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy Scholz, Gregor Mariana, Shinta Dharmawan, Agus Budi Syamsu, Iqbal Hörmann, Philipp Reuse, Carsten Hartmann, Jana Hiller, Karsten Prades, Joan Daniel Wasisto, Hutomo Suryo Waag, Andreas Sensors (Basel) Article Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility. MDPI 2019-03-11 /pmc/articles/PMC6427468/ /pubmed/30862101 http://dx.doi.org/10.3390/s19051234 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Scholz, Gregor
Mariana, Shinta
Dharmawan, Agus Budi
Syamsu, Iqbal
Hörmann, Philipp
Reuse, Carsten
Hartmann, Jana
Hiller, Karsten
Prades, Joan Daniel
Wasisto, Hutomo Suryo
Waag, Andreas
Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
title Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
title_full Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
title_fullStr Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
title_full_unstemmed Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
title_short Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
title_sort continuous live-cell culture imaging and single-cell tracking by computational lensfree led microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427468/
https://www.ncbi.nlm.nih.gov/pubmed/30862101
http://dx.doi.org/10.3390/s19051234
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