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Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy
Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effectiv...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427468/ https://www.ncbi.nlm.nih.gov/pubmed/30862101 http://dx.doi.org/10.3390/s19051234 |
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author | Scholz, Gregor Mariana, Shinta Dharmawan, Agus Budi Syamsu, Iqbal Hörmann, Philipp Reuse, Carsten Hartmann, Jana Hiller, Karsten Prades, Joan Daniel Wasisto, Hutomo Suryo Waag, Andreas |
author_facet | Scholz, Gregor Mariana, Shinta Dharmawan, Agus Budi Syamsu, Iqbal Hörmann, Philipp Reuse, Carsten Hartmann, Jana Hiller, Karsten Prades, Joan Daniel Wasisto, Hutomo Suryo Waag, Andreas |
author_sort | Scholz, Gregor |
collection | PubMed |
description | Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility. |
format | Online Article Text |
id | pubmed-6427468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64274682019-04-15 Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy Scholz, Gregor Mariana, Shinta Dharmawan, Agus Budi Syamsu, Iqbal Hörmann, Philipp Reuse, Carsten Hartmann, Jana Hiller, Karsten Prades, Joan Daniel Wasisto, Hutomo Suryo Waag, Andreas Sensors (Basel) Article Continuous cell culture monitoring as a way of investigating growth, proliferation, and kinetics of biological experiments is in high demand. However, commercially available solutions are typically expensive and large in size. Digital inline-holographic microscopes (DIHM) can provide a cost-effective alternative to conventional microscopes, bridging the gap towards live-cell culture imaging. In this work, a DIHM is built from inexpensive components and applied to different cell cultures. The images are reconstructed by computational methods and the data are analyzed with particle detection and tracking methods. Counting of cells as well as movement tracking of living cells is demonstrated, showing the feasibility of using a field-portable DIHM for basic cell culture investigation and bringing about the potential to deeply understand cell motility. MDPI 2019-03-11 /pmc/articles/PMC6427468/ /pubmed/30862101 http://dx.doi.org/10.3390/s19051234 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Scholz, Gregor Mariana, Shinta Dharmawan, Agus Budi Syamsu, Iqbal Hörmann, Philipp Reuse, Carsten Hartmann, Jana Hiller, Karsten Prades, Joan Daniel Wasisto, Hutomo Suryo Waag, Andreas Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
title | Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
title_full | Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
title_fullStr | Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
title_full_unstemmed | Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
title_short | Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy |
title_sort | continuous live-cell culture imaging and single-cell tracking by computational lensfree led microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427468/ https://www.ncbi.nlm.nih.gov/pubmed/30862101 http://dx.doi.org/10.3390/s19051234 |
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