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Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV as...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427804/ https://www.ncbi.nlm.nih.gov/pubmed/30818844 http://dx.doi.org/10.3390/ma12050704 |
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author | Piao, Mingyue Zou, Donglei Yang, Yuesuo Ren, Xianghao Qin, Chuanyu Piao, Yunxian |
author_facet | Piao, Mingyue Zou, Donglei Yang, Yuesuo Ren, Xianghao Qin, Chuanyu Piao, Yunxian |
author_sort | Piao, Mingyue |
collection | PubMed |
description | Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV assisted emulsion polymerization method followed by cross linking with glutaraldehyde (GA). The obtained Lac/particles were spherical and micron sized (137–535 μm), presenting high enzyme entrapment efficiency of 100%, high activity recovery of 18.9%, and great stability at various pHs (3–7) than the free Lac. The Lac/particles could adsorb the BPA into the catalytic particles in a short time, promoting contact between BPA and enzyme, and further enzymatically degrade them without the shaking process and independent surrounding buffer solution. The Lac/particles could be reused for another round BPA adsorption and biotranformation by maintaining over 90% of BPA removal efficiency after seven times reuse. The synergistic effects of adsorption and biocatalytical reaction of Lac/particles have significant values in high efficient and cost-effective BPA removal. |
format | Online Article Text |
id | pubmed-6427804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64278042019-04-10 Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A Piao, Mingyue Zou, Donglei Yang, Yuesuo Ren, Xianghao Qin, Chuanyu Piao, Yunxian Materials (Basel) Article Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV assisted emulsion polymerization method followed by cross linking with glutaraldehyde (GA). The obtained Lac/particles were spherical and micron sized (137–535 μm), presenting high enzyme entrapment efficiency of 100%, high activity recovery of 18.9%, and great stability at various pHs (3–7) than the free Lac. The Lac/particles could adsorb the BPA into the catalytic particles in a short time, promoting contact between BPA and enzyme, and further enzymatically degrade them without the shaking process and independent surrounding buffer solution. The Lac/particles could be reused for another round BPA adsorption and biotranformation by maintaining over 90% of BPA removal efficiency after seven times reuse. The synergistic effects of adsorption and biocatalytical reaction of Lac/particles have significant values in high efficient and cost-effective BPA removal. MDPI 2019-02-27 /pmc/articles/PMC6427804/ /pubmed/30818844 http://dx.doi.org/10.3390/ma12050704 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Piao, Mingyue Zou, Donglei Yang, Yuesuo Ren, Xianghao Qin, Chuanyu Piao, Yunxian Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A |
title | Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A |
title_full | Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A |
title_fullStr | Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A |
title_full_unstemmed | Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A |
title_short | Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A |
title_sort | multi-functional laccase immobilized hydrogel microparticles for efficient removal of bisphenol a |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427804/ https://www.ncbi.nlm.nih.gov/pubmed/30818844 http://dx.doi.org/10.3390/ma12050704 |
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