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Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A

Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV as...

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Autores principales: Piao, Mingyue, Zou, Donglei, Yang, Yuesuo, Ren, Xianghao, Qin, Chuanyu, Piao, Yunxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427804/
https://www.ncbi.nlm.nih.gov/pubmed/30818844
http://dx.doi.org/10.3390/ma12050704
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author Piao, Mingyue
Zou, Donglei
Yang, Yuesuo
Ren, Xianghao
Qin, Chuanyu
Piao, Yunxian
author_facet Piao, Mingyue
Zou, Donglei
Yang, Yuesuo
Ren, Xianghao
Qin, Chuanyu
Piao, Yunxian
author_sort Piao, Mingyue
collection PubMed
description Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV assisted emulsion polymerization method followed by cross linking with glutaraldehyde (GA). The obtained Lac/particles were spherical and micron sized (137–535 μm), presenting high enzyme entrapment efficiency of 100%, high activity recovery of 18.9%, and great stability at various pHs (3–7) than the free Lac. The Lac/particles could adsorb the BPA into the catalytic particles in a short time, promoting contact between BPA and enzyme, and further enzymatically degrade them without the shaking process and independent surrounding buffer solution. The Lac/particles could be reused for another round BPA adsorption and biotranformation by maintaining over 90% of BPA removal efficiency after seven times reuse. The synergistic effects of adsorption and biocatalytical reaction of Lac/particles have significant values in high efficient and cost-effective BPA removal.
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spelling pubmed-64278042019-04-10 Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A Piao, Mingyue Zou, Donglei Yang, Yuesuo Ren, Xianghao Qin, Chuanyu Piao, Yunxian Materials (Basel) Article Hghly stable, reusable, and multi-functional biocatalytic microparticles with Laccase (Lac) enzyme (Lac/particles) were synthesized for bisphenol A (BPA) removal from aqueous solution. The Lac/particles were prepared by encapsulating Lac enzymes into poly ethylene glycol (PEG) hydrogel via the UV assisted emulsion polymerization method followed by cross linking with glutaraldehyde (GA). The obtained Lac/particles were spherical and micron sized (137–535 μm), presenting high enzyme entrapment efficiency of 100%, high activity recovery of 18.9%, and great stability at various pHs (3–7) than the free Lac. The Lac/particles could adsorb the BPA into the catalytic particles in a short time, promoting contact between BPA and enzyme, and further enzymatically degrade them without the shaking process and independent surrounding buffer solution. The Lac/particles could be reused for another round BPA adsorption and biotranformation by maintaining over 90% of BPA removal efficiency after seven times reuse. The synergistic effects of adsorption and biocatalytical reaction of Lac/particles have significant values in high efficient and cost-effective BPA removal. MDPI 2019-02-27 /pmc/articles/PMC6427804/ /pubmed/30818844 http://dx.doi.org/10.3390/ma12050704 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Piao, Mingyue
Zou, Donglei
Yang, Yuesuo
Ren, Xianghao
Qin, Chuanyu
Piao, Yunxian
Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
title Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
title_full Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
title_fullStr Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
title_full_unstemmed Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
title_short Multi-Functional Laccase Immobilized Hydrogel Microparticles for Efficient Removal of Bisphenol A
title_sort multi-functional laccase immobilized hydrogel microparticles for efficient removal of bisphenol a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427804/
https://www.ncbi.nlm.nih.gov/pubmed/30818844
http://dx.doi.org/10.3390/ma12050704
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