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The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1

The outgrowth and metastasis of cervical cancer (CC) contribute to its malignancy. Pituitary Tumor Transforming Gene 1 (PTTG1) is upregulated in many types of cancer, and enhances tumor cell growth and metastasis. However, the activation and regulation of PTTG1 in CC, especially by its pseudogene PT...

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Autores principales: Guo, Xiang-cui, Li, Li, Gao, Zhi-hui, Zhou, Hong-wei, Li, Jun, Wang, Qian-qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428096/
https://www.ncbi.nlm.nih.gov/pubmed/30853662
http://dx.doi.org/10.18632/aging.101830
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author Guo, Xiang-cui
Li, Li
Gao, Zhi-hui
Zhou, Hong-wei
Li, Jun
Wang, Qian-qing
author_facet Guo, Xiang-cui
Li, Li
Gao, Zhi-hui
Zhou, Hong-wei
Li, Jun
Wang, Qian-qing
author_sort Guo, Xiang-cui
collection PubMed
description The outgrowth and metastasis of cervical cancer (CC) contribute to its malignancy. Pituitary Tumor Transforming Gene 1 (PTTG1) is upregulated in many types of cancer, and enhances tumor cell growth and metastasis. However, the activation and regulation of PTTG1 in CC, especially by its pseudogene PTTG3P, have not been shown. Here, we detected significantly higher levels of PTTG1 and PTTG3P in the resected CC tissue, compared to the paired adjacent normal cervical tissue. Interestingly, the PTTG3P levels positively correlated with the PTTG1 levels. High PTTG3P levels were associated with poor patients’ survival. In vitro, PTTG1 were increased by PTTG3P overexpression, but was inhibited by PTTG3P depletion in CC cells. However, PTTG3P levels were not altered by modulation of PTTG1 in CC cells, suggesting that PTTG3P is upstream of PTTG1. Moreover, PTTG3P increased CC cell growth, likely through CCNB1-mediated increase in cell proliferation, rather than through decrease in cell apoptosis. Furthermore, PTTG3P increased CC cell invasiveness, likely through upregulation of SNAIL and downregulation of E-cadherin. Our work thus suggests that PTTG3P may promote growth and metastasis of CC through PTTG1.
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spelling pubmed-64280962019-03-26 The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1 Guo, Xiang-cui Li, Li Gao, Zhi-hui Zhou, Hong-wei Li, Jun Wang, Qian-qing Aging (Albany NY) Research Paper The outgrowth and metastasis of cervical cancer (CC) contribute to its malignancy. Pituitary Tumor Transforming Gene 1 (PTTG1) is upregulated in many types of cancer, and enhances tumor cell growth and metastasis. However, the activation and regulation of PTTG1 in CC, especially by its pseudogene PTTG3P, have not been shown. Here, we detected significantly higher levels of PTTG1 and PTTG3P in the resected CC tissue, compared to the paired adjacent normal cervical tissue. Interestingly, the PTTG3P levels positively correlated with the PTTG1 levels. High PTTG3P levels were associated with poor patients’ survival. In vitro, PTTG1 were increased by PTTG3P overexpression, but was inhibited by PTTG3P depletion in CC cells. However, PTTG3P levels were not altered by modulation of PTTG1 in CC cells, suggesting that PTTG3P is upstream of PTTG1. Moreover, PTTG3P increased CC cell growth, likely through CCNB1-mediated increase in cell proliferation, rather than through decrease in cell apoptosis. Furthermore, PTTG3P increased CC cell invasiveness, likely through upregulation of SNAIL and downregulation of E-cadherin. Our work thus suggests that PTTG3P may promote growth and metastasis of CC through PTTG1. Impact Journals 2019-03-10 /pmc/articles/PMC6428096/ /pubmed/30853662 http://dx.doi.org/10.18632/aging.101830 Text en Copyright © 2019 Guo et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Guo, Xiang-cui
Li, Li
Gao, Zhi-hui
Zhou, Hong-wei
Li, Jun
Wang, Qian-qing
The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1
title The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1
title_full The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1
title_fullStr The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1
title_full_unstemmed The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1
title_short The long non-coding RNA PTTG3P promotes growth and metastasis of cervical cancer through PTTG1
title_sort long non-coding rna pttg3p promotes growth and metastasis of cervical cancer through pttg1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428096/
https://www.ncbi.nlm.nih.gov/pubmed/30853662
http://dx.doi.org/10.18632/aging.101830
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