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Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells

Blocking cytokine interleukin 10 (IL-10) at the time of immunisation enhances vaccine induced T cell responses and improves control of tumour cell growth in vivo. However, the effect of an IL-10 blockade on the biological function of macrophages has not been explored. In the current paper, a macroph...

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Autores principales: Ni, Guoying, Chen, Shu, Yuan, Jianwei, Cavezza, Shelley F., Wei, Ming Q., Li, Hejie, Pan, Xuan, Liu, Xiaosong, Wang, Tianfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428271/
https://www.ncbi.nlm.nih.gov/pubmed/30897137
http://dx.doi.org/10.1371/journal.pone.0213813
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author Ni, Guoying
Chen, Shu
Yuan, Jianwei
Cavezza, Shelley F.
Wei, Ming Q.
Li, Hejie
Pan, Xuan
Liu, Xiaosong
Wang, Tianfang
author_facet Ni, Guoying
Chen, Shu
Yuan, Jianwei
Cavezza, Shelley F.
Wei, Ming Q.
Li, Hejie
Pan, Xuan
Liu, Xiaosong
Wang, Tianfang
author_sort Ni, Guoying
collection PubMed
description Blocking cytokine interleukin 10 (IL-10) at the time of immunisation enhances vaccine induced T cell responses and improves control of tumour cell growth in vivo. However, the effect of an IL-10 blockade on the biological function of macrophages has not been explored. In the current paper, a macrophage precursor cell line, U937 cells, was selected to investigate the differential expression of proteins and relevant cell signalling pathway changes, when stimulated with lipopolysaccharide (LPS) in the presence of antibodies to IL-10 or IL-10 receptor. We used a quantitative proteomic strategy to investigate variations in protein profiles of U937 cells following the treatments with LPS, LPS plus human anti-IL10 antibody and anti-IL10R antibody in 24hrs, respectively. The LPS treatment significantly activated actin-related cell matrix formation and immune response pathways. The addition of anti-IL10 and anti-IL10R antibody further promoted the immune response and potentially effect macrophage survival through PI3K/AKT signalling; however, the latter appeared to also upregulated oncogene XRCC5 and Cajal body associated processes.
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spelling pubmed-64282712019-04-02 Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells Ni, Guoying Chen, Shu Yuan, Jianwei Cavezza, Shelley F. Wei, Ming Q. Li, Hejie Pan, Xuan Liu, Xiaosong Wang, Tianfang PLoS One Research Article Blocking cytokine interleukin 10 (IL-10) at the time of immunisation enhances vaccine induced T cell responses and improves control of tumour cell growth in vivo. However, the effect of an IL-10 blockade on the biological function of macrophages has not been explored. In the current paper, a macrophage precursor cell line, U937 cells, was selected to investigate the differential expression of proteins and relevant cell signalling pathway changes, when stimulated with lipopolysaccharide (LPS) in the presence of antibodies to IL-10 or IL-10 receptor. We used a quantitative proteomic strategy to investigate variations in protein profiles of U937 cells following the treatments with LPS, LPS plus human anti-IL10 antibody and anti-IL10R antibody in 24hrs, respectively. The LPS treatment significantly activated actin-related cell matrix formation and immune response pathways. The addition of anti-IL10 and anti-IL10R antibody further promoted the immune response and potentially effect macrophage survival through PI3K/AKT signalling; however, the latter appeared to also upregulated oncogene XRCC5 and Cajal body associated processes. Public Library of Science 2019-03-21 /pmc/articles/PMC6428271/ /pubmed/30897137 http://dx.doi.org/10.1371/journal.pone.0213813 Text en © 2019 Ni et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ni, Guoying
Chen, Shu
Yuan, Jianwei
Cavezza, Shelley F.
Wei, Ming Q.
Li, Hejie
Pan, Xuan
Liu, Xiaosong
Wang, Tianfang
Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
title Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
title_full Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
title_fullStr Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
title_full_unstemmed Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
title_short Comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-IL-10 and anti-IL-10 receptor antibodies in human U937 cells
title_sort comparative proteomic study reveals the enhanced immune response with the blockade of interleukin 10 with anti-il-10 and anti-il-10 receptor antibodies in human u937 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428271/
https://www.ncbi.nlm.nih.gov/pubmed/30897137
http://dx.doi.org/10.1371/journal.pone.0213813
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