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The transcriptome analysis of Protaetia brevitarsis Lewis larvae

Larvae of the pest Protaetia brevitarsis are used to treat infections in traditional Chinese medicine. However, genomic information about this non-model species is currently lacking. To better understand the fundamental biology of this non-model species, its transcriptome was obtained using next gen...

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Autores principales: Li, Zhongjie, Meng, Miaomiao, Li, Shasha, Deng, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428405/
https://www.ncbi.nlm.nih.gov/pubmed/30897120
http://dx.doi.org/10.1371/journal.pone.0214001
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author Li, Zhongjie
Meng, Miaomiao
Li, Shasha
Deng, Bo
author_facet Li, Zhongjie
Meng, Miaomiao
Li, Shasha
Deng, Bo
author_sort Li, Zhongjie
collection PubMed
description Larvae of the pest Protaetia brevitarsis are used to treat infections in traditional Chinese medicine. However, genomic information about this non-model species is currently lacking. To better understand the fundamental biology of this non-model species, its transcriptome was obtained using next generation sequencing and then analyzed. A total of 7.62 Gb of clean reads were obtained, which were assembled into 169,087 transcripts corresponding to 142,000 annotated unigenes. These unigenes were functionally classified according to Gene Ontology (GO), euKaryotic Ortholog Groups of proteins (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. A total of 41,921 unigenes were assigned to 56 GO terms, 21,454 unigenes were divided among 26 KOG categories, and 16,368 unigenes were assigned to 32 KEGG pathways. In addition, 19,144 simple sequence repeats (SSRs) were identified. Furthermore, several kinds of natural antimicrobial peptides and proteins, 4 histones with potential antimicrobial activity, and 41 potential antimicrobial peptide sequences were identified. These data are the first reported whole transcriptome sequence of P. brevitarsis larvae, which represents a valuable genomic resource for studying this species, thus promoting the utilization of its medical potential.
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spelling pubmed-64284052019-04-02 The transcriptome analysis of Protaetia brevitarsis Lewis larvae Li, Zhongjie Meng, Miaomiao Li, Shasha Deng, Bo PLoS One Research Article Larvae of the pest Protaetia brevitarsis are used to treat infections in traditional Chinese medicine. However, genomic information about this non-model species is currently lacking. To better understand the fundamental biology of this non-model species, its transcriptome was obtained using next generation sequencing and then analyzed. A total of 7.62 Gb of clean reads were obtained, which were assembled into 169,087 transcripts corresponding to 142,000 annotated unigenes. These unigenes were functionally classified according to Gene Ontology (GO), euKaryotic Ortholog Groups of proteins (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. A total of 41,921 unigenes were assigned to 56 GO terms, 21,454 unigenes were divided among 26 KOG categories, and 16,368 unigenes were assigned to 32 KEGG pathways. In addition, 19,144 simple sequence repeats (SSRs) were identified. Furthermore, several kinds of natural antimicrobial peptides and proteins, 4 histones with potential antimicrobial activity, and 41 potential antimicrobial peptide sequences were identified. These data are the first reported whole transcriptome sequence of P. brevitarsis larvae, which represents a valuable genomic resource for studying this species, thus promoting the utilization of its medical potential. Public Library of Science 2019-03-21 /pmc/articles/PMC6428405/ /pubmed/30897120 http://dx.doi.org/10.1371/journal.pone.0214001 Text en © 2019 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Zhongjie
Meng, Miaomiao
Li, Shasha
Deng, Bo
The transcriptome analysis of Protaetia brevitarsis Lewis larvae
title The transcriptome analysis of Protaetia brevitarsis Lewis larvae
title_full The transcriptome analysis of Protaetia brevitarsis Lewis larvae
title_fullStr The transcriptome analysis of Protaetia brevitarsis Lewis larvae
title_full_unstemmed The transcriptome analysis of Protaetia brevitarsis Lewis larvae
title_short The transcriptome analysis of Protaetia brevitarsis Lewis larvae
title_sort transcriptome analysis of protaetia brevitarsis lewis larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428405/
https://www.ncbi.nlm.nih.gov/pubmed/30897120
http://dx.doi.org/10.1371/journal.pone.0214001
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