Cargando…
Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells
BACKGROUND: Secondary metabolites from the group of isoprenoid compounds are widely distributed in mangrove plants. Polyisoprenoids (dolichol and polyprenol) are known to have benefits as anticancer agents. The present study was conducted to determine the cytotoxic potential of polyisoprenoids in le...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428521/ https://www.ncbi.nlm.nih.gov/pubmed/30583345 http://dx.doi.org/10.31557/APJCP.2018.19.12.3393 |
_version_ | 1783405409261846528 |
---|---|
author | Sari, Dini P Basyuni, Mohammad Hasibuan, Poppy AZ Sumardi, Sumardi Nuryawan, Arif Wati, Ridha |
author_facet | Sari, Dini P Basyuni, Mohammad Hasibuan, Poppy AZ Sumardi, Sumardi Nuryawan, Arif Wati, Ridha |
author_sort | Sari, Dini P |
collection | PubMed |
description | BACKGROUND: Secondary metabolites from the group of isoprenoid compounds are widely distributed in mangrove plants. Polyisoprenoids (dolichol and polyprenol) are known to have benefits as anticancer agents. The present study was conducted to determine the cytotoxic potential of polyisoprenoids in leaves from seventeen selected mangrove species against colon cancer (WiDr) cells. METHODS: Cytotoxic activity was evaluated by MTT assay in vitro using WiDr human colon cancer cells and 3T3 fibroblasts from Swiss albino mouse embryo tissue as controls. Mechanisms of action were approached by assessing apoptosis and the cell cycle using flow cytometry and fluorescence microscopy with annexin V-FITC, as well as expression of Bcl-2 and cyclin D1 by immunocytochemistry. RESULTS: Polyisoprenoids from N. fruticans leaves demonstrated the highest anticancer activity, with an IC(50) of 180.2 µg/mL, as compared to 397.7 µg/mL against 3T3 normal cells. Significant decrease in the expression of Bcl-2 and cyclin D1 was also noted, facilitating apoptosis and arrest of the cell cycle in the G0-G1 phase in WiDr cells. The present study showed for the first time that polyisoprenoids from N. fruticans exhibit concrete anticancer activity in vitro, decreasing cell proliferation and inducing apoptosis in colon cancer cells. CONCLUSIONS: Polyisoprenoids isolated from N. fruticans leaves may have promise as a source of anticancer agents. |
format | Online Article Text |
id | pubmed-6428521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-64285212019-04-01 Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells Sari, Dini P Basyuni, Mohammad Hasibuan, Poppy AZ Sumardi, Sumardi Nuryawan, Arif Wati, Ridha Asian Pac J Cancer Prev Research Article BACKGROUND: Secondary metabolites from the group of isoprenoid compounds are widely distributed in mangrove plants. Polyisoprenoids (dolichol and polyprenol) are known to have benefits as anticancer agents. The present study was conducted to determine the cytotoxic potential of polyisoprenoids in leaves from seventeen selected mangrove species against colon cancer (WiDr) cells. METHODS: Cytotoxic activity was evaluated by MTT assay in vitro using WiDr human colon cancer cells and 3T3 fibroblasts from Swiss albino mouse embryo tissue as controls. Mechanisms of action were approached by assessing apoptosis and the cell cycle using flow cytometry and fluorescence microscopy with annexin V-FITC, as well as expression of Bcl-2 and cyclin D1 by immunocytochemistry. RESULTS: Polyisoprenoids from N. fruticans leaves demonstrated the highest anticancer activity, with an IC(50) of 180.2 µg/mL, as compared to 397.7 µg/mL against 3T3 normal cells. Significant decrease in the expression of Bcl-2 and cyclin D1 was also noted, facilitating apoptosis and arrest of the cell cycle in the G0-G1 phase in WiDr cells. The present study showed for the first time that polyisoprenoids from N. fruticans exhibit concrete anticancer activity in vitro, decreasing cell proliferation and inducing apoptosis in colon cancer cells. CONCLUSIONS: Polyisoprenoids isolated from N. fruticans leaves may have promise as a source of anticancer agents. West Asia Organization for Cancer Prevention 2018 /pmc/articles/PMC6428521/ /pubmed/30583345 http://dx.doi.org/10.31557/APJCP.2018.19.12.3393 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License |
spellingShingle | Research Article Sari, Dini P Basyuni, Mohammad Hasibuan, Poppy AZ Sumardi, Sumardi Nuryawan, Arif Wati, Ridha Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells |
title | Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells |
title_full | Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells |
title_fullStr | Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells |
title_full_unstemmed | Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells |
title_short | Cytotoxic and Antiproliferative Activity of Polyisoprenoids in Seventeen Mangroves Species Against WiDr Colon Cancer Cells |
title_sort | cytotoxic and antiproliferative activity of polyisoprenoids in seventeen mangroves species against widr colon cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428521/ https://www.ncbi.nlm.nih.gov/pubmed/30583345 http://dx.doi.org/10.31557/APJCP.2018.19.12.3393 |
work_keys_str_mv | AT saridinip cytotoxicandantiproliferativeactivityofpolyisoprenoidsinseventeenmangrovesspeciesagainstwidrcoloncancercells AT basyunimohammad cytotoxicandantiproliferativeactivityofpolyisoprenoidsinseventeenmangrovesspeciesagainstwidrcoloncancercells AT hasibuanpoppyaz cytotoxicandantiproliferativeactivityofpolyisoprenoidsinseventeenmangrovesspeciesagainstwidrcoloncancercells AT sumardisumardi cytotoxicandantiproliferativeactivityofpolyisoprenoidsinseventeenmangrovesspeciesagainstwidrcoloncancercells AT nuryawanarif cytotoxicandantiproliferativeactivityofpolyisoprenoidsinseventeenmangrovesspeciesagainstwidrcoloncancercells AT watiridha cytotoxicandantiproliferativeactivityofpolyisoprenoidsinseventeenmangrovesspeciesagainstwidrcoloncancercells |