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Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria

Abstract. Longidoruspolyaesp. n., a bisexual nematode species found in the rhizosphere of pear tree (Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8–9.1 mm; a comparatively long odontostyle (114.0–127.5 μm); a narrow li...

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Autores principales: Lazarova, Stela S., Elshishka, Milka, Radoslavov, Georgi, Lozanova, Lydmila, Hristov, Peter, Mladenov, Alexander, Zheng, Jingwu, Fanelli, Elena, Francesca De Luca, Peneva, Vlada K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428786/
https://www.ncbi.nlm.nih.gov/pubmed/30918443
http://dx.doi.org/10.3897/zookeys.830.32188
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author Lazarova, Stela S.
Elshishka, Milka
Radoslavov, Georgi
Lozanova, Lydmila
Hristov, Peter
Mladenov, Alexander
Zheng, Jingwu
Fanelli, Elena
Francesca De Luca,
Peneva, Vlada K.
author_facet Lazarova, Stela S.
Elshishka, Milka
Radoslavov, Georgi
Lozanova, Lydmila
Hristov, Peter
Mladenov, Alexander
Zheng, Jingwu
Fanelli, Elena
Francesca De Luca,
Peneva, Vlada K.
author_sort Lazarova, Stela S.
collection PubMed
description Abstract. Longidoruspolyaesp. n., a bisexual nematode species found in the rhizosphere of pear tree (Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8–9.1 mm; a comparatively long odontostyle (114.0–127.5 μm); a narrow lip region (14.0–15.5 μm), anteriorly flattened and almost continuous with the body profile; pocket-like amphidial pouches long, deeply bilobed, and slightly asymmetrical, a guide ring at 37–42 μm from the anterior end; normal arrangement of pharyngeal glands; and a short bluntly rounded to hemispherical tail. Four juvenile stages identified: the first stage with a digitate tail, and the second and subsequent stages with a bluntly rounded tail. Males have one adcloacal pair and a row of 10 or 11 single ventromedian supplements; spicules 71.0–74.5 μm long. Based on morphometric data, the new species belongs to a group of species spread over Europe (L.arthensis, L.silvae, L.uroshis,), Iran (L.kheirii), and Syria (L.pauli), which share common characters such as amphidial fovea, lip region and tail shapes, similar odontostyle and body length, and similar first-stage juvenile tail shape. Codes for identifying the new species are A5, B2, C34, D3, E3, F45, G12, H1, I2, J1, K7. The phylogenetic analysis based on D2-D3 expansion domains of the rRNA gene revealed that the new species has the closest relationships with L.athesinus from Italy and three unidentified Longidorus spp. from USA (Longidorus sp. 1, Longidorus sp. 2, and Longidorus sp. 6). New morphometric and molecular data (18S rRNA gene, ITS1-5.8S-ITS2 regions and D2-D3 28S rRNA gene sequences) for three populations of L.pisi from Bulgaria were obtained and variations between populations are discussed.
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spelling pubmed-64287862019-03-27 Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria Lazarova, Stela S. Elshishka, Milka Radoslavov, Georgi Lozanova, Lydmila Hristov, Peter Mladenov, Alexander Zheng, Jingwu Fanelli, Elena Francesca De Luca, Peneva, Vlada K. Zookeys Research Article Abstract. Longidoruspolyaesp. n., a bisexual nematode species found in the rhizosphere of pear tree (Pyruscommunis L.), is described and characterised using an integrative approach. The new species has a female body length of 6.8–9.1 mm; a comparatively long odontostyle (114.0–127.5 μm); a narrow lip region (14.0–15.5 μm), anteriorly flattened and almost continuous with the body profile; pocket-like amphidial pouches long, deeply bilobed, and slightly asymmetrical, a guide ring at 37–42 μm from the anterior end; normal arrangement of pharyngeal glands; and a short bluntly rounded to hemispherical tail. Four juvenile stages identified: the first stage with a digitate tail, and the second and subsequent stages with a bluntly rounded tail. Males have one adcloacal pair and a row of 10 or 11 single ventromedian supplements; spicules 71.0–74.5 μm long. Based on morphometric data, the new species belongs to a group of species spread over Europe (L.arthensis, L.silvae, L.uroshis,), Iran (L.kheirii), and Syria (L.pauli), which share common characters such as amphidial fovea, lip region and tail shapes, similar odontostyle and body length, and similar first-stage juvenile tail shape. Codes for identifying the new species are A5, B2, C34, D3, E3, F45, G12, H1, I2, J1, K7. The phylogenetic analysis based on D2-D3 expansion domains of the rRNA gene revealed that the new species has the closest relationships with L.athesinus from Italy and three unidentified Longidorus spp. from USA (Longidorus sp. 1, Longidorus sp. 2, and Longidorus sp. 6). New morphometric and molecular data (18S rRNA gene, ITS1-5.8S-ITS2 regions and D2-D3 28S rRNA gene sequences) for three populations of L.pisi from Bulgaria were obtained and variations between populations are discussed. Pensoft Publishers 2019-03-14 /pmc/articles/PMC6428786/ /pubmed/30918443 http://dx.doi.org/10.3897/zookeys.830.32188 Text en Stela S. Lazarova, Milka Elshishka, Georgi Radoslavov, Lydmila Lozanova, Peter Hristov, Alexander Mladenov, Jingwu Zheng, Elena Fanelli, Francesca De Luca, Vlada K. Peneva http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lazarova, Stela S.
Elshishka, Milka
Radoslavov, Georgi
Lozanova, Lydmila
Hristov, Peter
Mladenov, Alexander
Zheng, Jingwu
Fanelli, Elena
Francesca De Luca,
Peneva, Vlada K.
Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria
title Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria
title_full Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria
title_fullStr Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria
title_full_unstemmed Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria
title_short Molecular and morphological characterisation of Longidoruspolyae sp. n. and L.pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria
title_sort molecular and morphological characterisation of longidoruspolyae sp. n. and l.pisi edward, misra & singh, 1964 (dorylaimida, longidoridae) from bulgaria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428786/
https://www.ncbi.nlm.nih.gov/pubmed/30918443
http://dx.doi.org/10.3897/zookeys.830.32188
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