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Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats
Synaptophysin (SYP) and growth-associated binding protein 43 (GAP-43) have been shown to be closely related to hippocampal synaptic plasticity in recent years. They are important molecular markers associated with synaptic plasticity. However, the role of SYP and GAP-43 in chronic intermittent hypoxi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428973/ https://www.ncbi.nlm.nih.gov/pubmed/30911273 http://dx.doi.org/10.7150/ijms.28359 |
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author | Zhu, Xiankun Wang, Pei Liu, Haijun Zhan, Jing Wang, Jin Li, Mi Zeng, Ling Xu, Ping |
author_facet | Zhu, Xiankun Wang, Pei Liu, Haijun Zhan, Jing Wang, Jin Li, Mi Zeng, Ling Xu, Ping |
author_sort | Zhu, Xiankun |
collection | PubMed |
description | Synaptophysin (SYP) and growth-associated binding protein 43 (GAP-43) have been shown to be closely related to hippocampal synaptic plasticity in recent years. They are important molecular markers associated with synaptic plasticity. However, the role of SYP and GAP-43 in chronic intermittent hypoxic injury of the central nervous system needs to be further clarified. In this study, 25 adult male sprague dawley (SD) rats were randomly divided into a normal control group (CON) and a chronic intermittent hypoxia group (CIH) with four time points as follows: 1 W, 2 W, 3 W, and 4 W. The behavioural changes (primarily learning and memory abilities) were observed by the Morris water maze in each group, consisting of 5 rats per group.The localization of SYP and GAP-43 in hippocampal CA1 neurons was observed, and the expression of SYP and GAP-43 in the hippocampus was detected by Western blotting. The results showed that the mean oxygen saturation of the tail artery in CIH rats was less than that in normal rats (P < 0.05). The escape latency of CIH rats was longer than that of normal rats, and the number of space exploration platform crossings was less than that of normal rats. SYP-positive stained cells were yellow or brown and were mainly expressed on the cell membrane, while the GAP-43-positive staining was brown and was mainly expressed on the cell membrane and in the cytoplasm. The expression of SYP in plasma decreased gradually at the four time points for the CIH group (P < 0.05), while the expression of GAP-43 in the CIH 1W group increased (P < 0.05) and decreased gradually in the CIH 2 W, CIH 3 W and CIH 4 W groups (P < 0.05). |
format | Online Article Text |
id | pubmed-6428973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-64289732019-03-25 Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats Zhu, Xiankun Wang, Pei Liu, Haijun Zhan, Jing Wang, Jin Li, Mi Zeng, Ling Xu, Ping Int J Med Sci Research Paper Synaptophysin (SYP) and growth-associated binding protein 43 (GAP-43) have been shown to be closely related to hippocampal synaptic plasticity in recent years. They are important molecular markers associated with synaptic plasticity. However, the role of SYP and GAP-43 in chronic intermittent hypoxic injury of the central nervous system needs to be further clarified. In this study, 25 adult male sprague dawley (SD) rats were randomly divided into a normal control group (CON) and a chronic intermittent hypoxia group (CIH) with four time points as follows: 1 W, 2 W, 3 W, and 4 W. The behavioural changes (primarily learning and memory abilities) were observed by the Morris water maze in each group, consisting of 5 rats per group.The localization of SYP and GAP-43 in hippocampal CA1 neurons was observed, and the expression of SYP and GAP-43 in the hippocampus was detected by Western blotting. The results showed that the mean oxygen saturation of the tail artery in CIH rats was less than that in normal rats (P < 0.05). The escape latency of CIH rats was longer than that of normal rats, and the number of space exploration platform crossings was less than that of normal rats. SYP-positive stained cells were yellow or brown and were mainly expressed on the cell membrane, while the GAP-43-positive staining was brown and was mainly expressed on the cell membrane and in the cytoplasm. The expression of SYP in plasma decreased gradually at the four time points for the CIH group (P < 0.05), while the expression of GAP-43 in the CIH 1W group increased (P < 0.05) and decreased gradually in the CIH 2 W, CIH 3 W and CIH 4 W groups (P < 0.05). Ivyspring International Publisher 2019-01-29 /pmc/articles/PMC6428973/ /pubmed/30911273 http://dx.doi.org/10.7150/ijms.28359 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Zhu, Xiankun Wang, Pei Liu, Haijun Zhan, Jing Wang, Jin Li, Mi Zeng, Ling Xu, Ping Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats |
title | Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats |
title_full | Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats |
title_fullStr | Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats |
title_full_unstemmed | Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats |
title_short | Changes and Significance of SYP and GAP-43 Expression in the Hippocampus of CIH Rats |
title_sort | changes and significance of syp and gap-43 expression in the hippocampus of cih rats |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428973/ https://www.ncbi.nlm.nih.gov/pubmed/30911273 http://dx.doi.org/10.7150/ijms.28359 |
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