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Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
Mid-to-late gestation is a unique period in which women experience dynamic changes in lipid metabolism. Although the recent intensive epigenome-wide association studies (EWAS) using peripheral leukocytes have revealed that lipid-related traits alter DNA methylation, the influence of pregnancy-induce...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429071/ https://www.ncbi.nlm.nih.gov/pubmed/30823689 http://dx.doi.org/10.3390/ijms20051066 |
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author | Pavethynath, Shilpa Imai, Chihiro Jin, Xin Hichiwa, Naomi Takimoto, Hidemi Okamitsu, Motoko Tarui, Iori Aoyama, Tomoko Yago, Satoshi Fudono, Ayako Muramatsu, Masaaki Miyasaka, Naoyuki Sato, Noriko |
author_facet | Pavethynath, Shilpa Imai, Chihiro Jin, Xin Hichiwa, Naomi Takimoto, Hidemi Okamitsu, Motoko Tarui, Iori Aoyama, Tomoko Yago, Satoshi Fudono, Ayako Muramatsu, Masaaki Miyasaka, Naoyuki Sato, Noriko |
author_sort | Pavethynath, Shilpa |
collection | PubMed |
description | Mid-to-late gestation is a unique period in which women experience dynamic changes in lipid metabolism. Although the recent intensive epigenome-wide association studies (EWAS) using peripheral leukocytes have revealed that lipid-related traits alter DNA methylation, the influence of pregnancy-induced metabolic changes on the methylation levels of these differentially methylated sites is not well known. In this study, we performed a prospective cohort study of pregnant women (n = 52) using the MassARRAY EpiTYPER assay and analyzed the methylation levels of variably methylated sites, including CPT1A intron 1 and SREBF1 intron 1 CpGs, which were previously verified to be robustly associated with adiposity traits. Although methylation of SREBF1 was associated with body mass index (BMI) and low-density lipoprotein cholesterol at mid-gestation, this association was attenuated at late gestation, which was consistent with the metabolic switch from an anabolic to a catabolic state. However, the BMI association with CPT1A intron 1 methylation appeared to strengthen at late gestation; this association was mediated by pre-pregnancy BMI-dependent change in the leukocyte proportion during mid-to-late gestation. Thus, the methylation of adiposity-related differentially methylated regions was sensitive to metabolic and immunological changes during mid-to-late gestation. |
format | Online Article Text |
id | pubmed-6429071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64290712019-04-10 Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 Pavethynath, Shilpa Imai, Chihiro Jin, Xin Hichiwa, Naomi Takimoto, Hidemi Okamitsu, Motoko Tarui, Iori Aoyama, Tomoko Yago, Satoshi Fudono, Ayako Muramatsu, Masaaki Miyasaka, Naoyuki Sato, Noriko Int J Mol Sci Article Mid-to-late gestation is a unique period in which women experience dynamic changes in lipid metabolism. Although the recent intensive epigenome-wide association studies (EWAS) using peripheral leukocytes have revealed that lipid-related traits alter DNA methylation, the influence of pregnancy-induced metabolic changes on the methylation levels of these differentially methylated sites is not well known. In this study, we performed a prospective cohort study of pregnant women (n = 52) using the MassARRAY EpiTYPER assay and analyzed the methylation levels of variably methylated sites, including CPT1A intron 1 and SREBF1 intron 1 CpGs, which were previously verified to be robustly associated with adiposity traits. Although methylation of SREBF1 was associated with body mass index (BMI) and low-density lipoprotein cholesterol at mid-gestation, this association was attenuated at late gestation, which was consistent with the metabolic switch from an anabolic to a catabolic state. However, the BMI association with CPT1A intron 1 methylation appeared to strengthen at late gestation; this association was mediated by pre-pregnancy BMI-dependent change in the leukocyte proportion during mid-to-late gestation. Thus, the methylation of adiposity-related differentially methylated regions was sensitive to metabolic and immunological changes during mid-to-late gestation. MDPI 2019-03-01 /pmc/articles/PMC6429071/ /pubmed/30823689 http://dx.doi.org/10.3390/ijms20051066 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pavethynath, Shilpa Imai, Chihiro Jin, Xin Hichiwa, Naomi Takimoto, Hidemi Okamitsu, Motoko Tarui, Iori Aoyama, Tomoko Yago, Satoshi Fudono, Ayako Muramatsu, Masaaki Miyasaka, Naoyuki Sato, Noriko Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 |
title | Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 |
title_full | Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 |
title_fullStr | Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 |
title_full_unstemmed | Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 |
title_short | Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 |
title_sort | metabolic and immunological shifts during mid-to-late gestation influence maternal blood methylation of cpt1a and srebf1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429071/ https://www.ncbi.nlm.nih.gov/pubmed/30823689 http://dx.doi.org/10.3390/ijms20051066 |
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