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Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1

Mid-to-late gestation is a unique period in which women experience dynamic changes in lipid metabolism. Although the recent intensive epigenome-wide association studies (EWAS) using peripheral leukocytes have revealed that lipid-related traits alter DNA methylation, the influence of pregnancy-induce...

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Autores principales: Pavethynath, Shilpa, Imai, Chihiro, Jin, Xin, Hichiwa, Naomi, Takimoto, Hidemi, Okamitsu, Motoko, Tarui, Iori, Aoyama, Tomoko, Yago, Satoshi, Fudono, Ayako, Muramatsu, Masaaki, Miyasaka, Naoyuki, Sato, Noriko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429071/
https://www.ncbi.nlm.nih.gov/pubmed/30823689
http://dx.doi.org/10.3390/ijms20051066
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author Pavethynath, Shilpa
Imai, Chihiro
Jin, Xin
Hichiwa, Naomi
Takimoto, Hidemi
Okamitsu, Motoko
Tarui, Iori
Aoyama, Tomoko
Yago, Satoshi
Fudono, Ayako
Muramatsu, Masaaki
Miyasaka, Naoyuki
Sato, Noriko
author_facet Pavethynath, Shilpa
Imai, Chihiro
Jin, Xin
Hichiwa, Naomi
Takimoto, Hidemi
Okamitsu, Motoko
Tarui, Iori
Aoyama, Tomoko
Yago, Satoshi
Fudono, Ayako
Muramatsu, Masaaki
Miyasaka, Naoyuki
Sato, Noriko
author_sort Pavethynath, Shilpa
collection PubMed
description Mid-to-late gestation is a unique period in which women experience dynamic changes in lipid metabolism. Although the recent intensive epigenome-wide association studies (EWAS) using peripheral leukocytes have revealed that lipid-related traits alter DNA methylation, the influence of pregnancy-induced metabolic changes on the methylation levels of these differentially methylated sites is not well known. In this study, we performed a prospective cohort study of pregnant women (n = 52) using the MassARRAY EpiTYPER assay and analyzed the methylation levels of variably methylated sites, including CPT1A intron 1 and SREBF1 intron 1 CpGs, which were previously verified to be robustly associated with adiposity traits. Although methylation of SREBF1 was associated with body mass index (BMI) and low-density lipoprotein cholesterol at mid-gestation, this association was attenuated at late gestation, which was consistent with the metabolic switch from an anabolic to a catabolic state. However, the BMI association with CPT1A intron 1 methylation appeared to strengthen at late gestation; this association was mediated by pre-pregnancy BMI-dependent change in the leukocyte proportion during mid-to-late gestation. Thus, the methylation of adiposity-related differentially methylated regions was sensitive to metabolic and immunological changes during mid-to-late gestation.
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spelling pubmed-64290712019-04-10 Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1 Pavethynath, Shilpa Imai, Chihiro Jin, Xin Hichiwa, Naomi Takimoto, Hidemi Okamitsu, Motoko Tarui, Iori Aoyama, Tomoko Yago, Satoshi Fudono, Ayako Muramatsu, Masaaki Miyasaka, Naoyuki Sato, Noriko Int J Mol Sci Article Mid-to-late gestation is a unique period in which women experience dynamic changes in lipid metabolism. Although the recent intensive epigenome-wide association studies (EWAS) using peripheral leukocytes have revealed that lipid-related traits alter DNA methylation, the influence of pregnancy-induced metabolic changes on the methylation levels of these differentially methylated sites is not well known. In this study, we performed a prospective cohort study of pregnant women (n = 52) using the MassARRAY EpiTYPER assay and analyzed the methylation levels of variably methylated sites, including CPT1A intron 1 and SREBF1 intron 1 CpGs, which were previously verified to be robustly associated with adiposity traits. Although methylation of SREBF1 was associated with body mass index (BMI) and low-density lipoprotein cholesterol at mid-gestation, this association was attenuated at late gestation, which was consistent with the metabolic switch from an anabolic to a catabolic state. However, the BMI association with CPT1A intron 1 methylation appeared to strengthen at late gestation; this association was mediated by pre-pregnancy BMI-dependent change in the leukocyte proportion during mid-to-late gestation. Thus, the methylation of adiposity-related differentially methylated regions was sensitive to metabolic and immunological changes during mid-to-late gestation. MDPI 2019-03-01 /pmc/articles/PMC6429071/ /pubmed/30823689 http://dx.doi.org/10.3390/ijms20051066 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pavethynath, Shilpa
Imai, Chihiro
Jin, Xin
Hichiwa, Naomi
Takimoto, Hidemi
Okamitsu, Motoko
Tarui, Iori
Aoyama, Tomoko
Yago, Satoshi
Fudono, Ayako
Muramatsu, Masaaki
Miyasaka, Naoyuki
Sato, Noriko
Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
title Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
title_full Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
title_fullStr Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
title_full_unstemmed Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
title_short Metabolic and Immunological Shifts during Mid-to-Late Gestation Influence Maternal Blood Methylation of CPT1A and SREBF1
title_sort metabolic and immunological shifts during mid-to-late gestation influence maternal blood methylation of cpt1a and srebf1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429071/
https://www.ncbi.nlm.nih.gov/pubmed/30823689
http://dx.doi.org/10.3390/ijms20051066
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