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Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori

Wnt is a family of conserved glycoproteins that participate in a variety of important biological processes including embryo development, cell proliferation and differentiation, and tissue regeneration. The Wnt family is a metazoan novelty found in all animal phyla. Studies have revealed that the num...

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Autores principales: Ding, Xin, Liu, Junxia, Zheng, Lu, Song, Jiangbo, Li, Niannian, Hu, Hai, Tong, Xiaoling, Dai, Fangyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429082/
https://www.ncbi.nlm.nih.gov/pubmed/30862048
http://dx.doi.org/10.3390/ijms20051221
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author Ding, Xin
Liu, Junxia
Zheng, Lu
Song, Jiangbo
Li, Niannian
Hu, Hai
Tong, Xiaoling
Dai, Fangyin
author_facet Ding, Xin
Liu, Junxia
Zheng, Lu
Song, Jiangbo
Li, Niannian
Hu, Hai
Tong, Xiaoling
Dai, Fangyin
author_sort Ding, Xin
collection PubMed
description Wnt is a family of conserved glycoproteins that participate in a variety of important biological processes including embryo development, cell proliferation and differentiation, and tissue regeneration. The Wnt family is a metazoan novelty found in all animal phyla. Studies have revealed that the number of Wnt genes varies among species, presumably due to reproduction and loss of genes during evolution. However, a comprehensive inventory of Wnt genes in Lepidoptera is lacking. In this study, we identified the repertoire of Wnt genes in the silkworm and seven other species of Lepidoptera and obtained eight Wnt genes (Wnt1, Wnt5–Wnt7, Wnt9–Wnt11, and WntA) in each species. Four of these Wnt genes are clustered in two orientations (5′-Wnt9-Wnt1-Wnt6-Wnt10-3′ and 5′-Wnt10-Wnt6-Wnt1-Wnt9-3′) in both moths and butterflies. Transcript analysis of Wnt in silkworm embryonic stages showed that each BmWnt gene had a unique expression pattern during embryological development. Analysis of a larval stage revealed differential expression of Wnt family members in diverse tissues. Our study provides an overview of the Wnt family in Lepidoptera and will inspire further functional study of the Wnt genes in the silkworm.
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spelling pubmed-64290822019-04-10 Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori Ding, Xin Liu, Junxia Zheng, Lu Song, Jiangbo Li, Niannian Hu, Hai Tong, Xiaoling Dai, Fangyin Int J Mol Sci Article Wnt is a family of conserved glycoproteins that participate in a variety of important biological processes including embryo development, cell proliferation and differentiation, and tissue regeneration. The Wnt family is a metazoan novelty found in all animal phyla. Studies have revealed that the number of Wnt genes varies among species, presumably due to reproduction and loss of genes during evolution. However, a comprehensive inventory of Wnt genes in Lepidoptera is lacking. In this study, we identified the repertoire of Wnt genes in the silkworm and seven other species of Lepidoptera and obtained eight Wnt genes (Wnt1, Wnt5–Wnt7, Wnt9–Wnt11, and WntA) in each species. Four of these Wnt genes are clustered in two orientations (5′-Wnt9-Wnt1-Wnt6-Wnt10-3′ and 5′-Wnt10-Wnt6-Wnt1-Wnt9-3′) in both moths and butterflies. Transcript analysis of Wnt in silkworm embryonic stages showed that each BmWnt gene had a unique expression pattern during embryological development. Analysis of a larval stage revealed differential expression of Wnt family members in diverse tissues. Our study provides an overview of the Wnt family in Lepidoptera and will inspire further functional study of the Wnt genes in the silkworm. MDPI 2019-03-11 /pmc/articles/PMC6429082/ /pubmed/30862048 http://dx.doi.org/10.3390/ijms20051221 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Xin
Liu, Junxia
Zheng, Lu
Song, Jiangbo
Li, Niannian
Hu, Hai
Tong, Xiaoling
Dai, Fangyin
Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
title Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
title_full Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
title_fullStr Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
title_full_unstemmed Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
title_short Genome-Wide Identification and Expression Profiling of Wnt Family Genes in the Silkworm, Bombyx mori
title_sort genome-wide identification and expression profiling of wnt family genes in the silkworm, bombyx mori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429082/
https://www.ncbi.nlm.nih.gov/pubmed/30862048
http://dx.doi.org/10.3390/ijms20051221
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