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Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells

A deeper understanding of the detailed mechanism of in vivo tissue healing is necessary for the development of novel regenerative therapies. Among several external factors, environmental pH is one of the crucial parameters that greatly affects enzyme activity and cellular biochemical reactions invol...

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Autores principales: Hazehara-Kunitomo, Yuri, Hara, Emilio Satoshi, Ono, Mitsuaki, Aung, Kyaw Thu, Komi, Keiko, Pham, Hai Thanh, Akiyama, Kentaro, Okada, Masahiro, Oohashi, Toshitaka, Matsumoto, Takuya, Kuboki, Takuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429188/
https://www.ncbi.nlm.nih.gov/pubmed/30836626
http://dx.doi.org/10.3390/ijms20051097
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author Hazehara-Kunitomo, Yuri
Hara, Emilio Satoshi
Ono, Mitsuaki
Aung, Kyaw Thu
Komi, Keiko
Pham, Hai Thanh
Akiyama, Kentaro
Okada, Masahiro
Oohashi, Toshitaka
Matsumoto, Takuya
Kuboki, Takuo
author_facet Hazehara-Kunitomo, Yuri
Hara, Emilio Satoshi
Ono, Mitsuaki
Aung, Kyaw Thu
Komi, Keiko
Pham, Hai Thanh
Akiyama, Kentaro
Okada, Masahiro
Oohashi, Toshitaka
Matsumoto, Takuya
Kuboki, Takuo
author_sort Hazehara-Kunitomo, Yuri
collection PubMed
description A deeper understanding of the detailed mechanism of in vivo tissue healing is necessary for the development of novel regenerative therapies. Among several external factors, environmental pH is one of the crucial parameters that greatly affects enzyme activity and cellular biochemical reactions involving tissue repair and homeostasis. In this study, in order to analyze the microenvironmental conditions during bone healing, we first measured the pH in vivo at the bone healing site using a high-resolution fiber optic pH microsensor directly in femur defects and tooth extraction sockets. The pH was shown to decrease from physiological 7.4 to 6.8 during the initial two days of healing (inflammatory phase). In the same initial stages of the inflammatory phase of the bone healing process, mesenchymal stem cells (MSCs) are known to migrate to the healing site to contribute to tissue repair. Therefore, we investigated the effect of a short-term acidic (pH 6.8) pre-treatment on the stemness of bone marrow-derived MSCs (BMSCs). Interestingly, the results showed that pre-treatment of BMSCs with acidic pH enhances the expression of stem cell markers (OCT-4, NANOG, SSEA-4), as well as cell viability and proliferation. On the other hand, acidic pH decreased BMSC migration ability. These results indicate that acidic pH during the initial stages of bone healing is important to enhance the stem cell properties of BMSCs. These findings may enable the development of novel methods for optimization of stem cell function towards tissue engineering or regenerative medicine.
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spelling pubmed-64291882019-04-10 Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells Hazehara-Kunitomo, Yuri Hara, Emilio Satoshi Ono, Mitsuaki Aung, Kyaw Thu Komi, Keiko Pham, Hai Thanh Akiyama, Kentaro Okada, Masahiro Oohashi, Toshitaka Matsumoto, Takuya Kuboki, Takuo Int J Mol Sci Communication A deeper understanding of the detailed mechanism of in vivo tissue healing is necessary for the development of novel regenerative therapies. Among several external factors, environmental pH is one of the crucial parameters that greatly affects enzyme activity and cellular biochemical reactions involving tissue repair and homeostasis. In this study, in order to analyze the microenvironmental conditions during bone healing, we first measured the pH in vivo at the bone healing site using a high-resolution fiber optic pH microsensor directly in femur defects and tooth extraction sockets. The pH was shown to decrease from physiological 7.4 to 6.8 during the initial two days of healing (inflammatory phase). In the same initial stages of the inflammatory phase of the bone healing process, mesenchymal stem cells (MSCs) are known to migrate to the healing site to contribute to tissue repair. Therefore, we investigated the effect of a short-term acidic (pH 6.8) pre-treatment on the stemness of bone marrow-derived MSCs (BMSCs). Interestingly, the results showed that pre-treatment of BMSCs with acidic pH enhances the expression of stem cell markers (OCT-4, NANOG, SSEA-4), as well as cell viability and proliferation. On the other hand, acidic pH decreased BMSC migration ability. These results indicate that acidic pH during the initial stages of bone healing is important to enhance the stem cell properties of BMSCs. These findings may enable the development of novel methods for optimization of stem cell function towards tissue engineering or regenerative medicine. MDPI 2019-03-04 /pmc/articles/PMC6429188/ /pubmed/30836626 http://dx.doi.org/10.3390/ijms20051097 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Hazehara-Kunitomo, Yuri
Hara, Emilio Satoshi
Ono, Mitsuaki
Aung, Kyaw Thu
Komi, Keiko
Pham, Hai Thanh
Akiyama, Kentaro
Okada, Masahiro
Oohashi, Toshitaka
Matsumoto, Takuya
Kuboki, Takuo
Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells
title Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells
title_full Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells
title_fullStr Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells
title_full_unstemmed Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells
title_short Acidic Pre-Conditioning Enhances the Stem Cell Phenotype of Human Bone Marrow Stem/Progenitor Cells
title_sort acidic pre-conditioning enhances the stem cell phenotype of human bone marrow stem/progenitor cells
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429188/
https://www.ncbi.nlm.nih.gov/pubmed/30836626
http://dx.doi.org/10.3390/ijms20051097
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