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Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach
Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable fo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429194/ https://www.ncbi.nlm.nih.gov/pubmed/30823685 http://dx.doi.org/10.3390/molecules24050871 |
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author | Santos-Rosales, Víctor Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. |
author_facet | Santos-Rosales, Víctor Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. |
author_sort | Santos-Rosales, Víctor |
collection | PubMed |
description | Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein:starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO(2) sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications. |
format | Online Article Text |
id | pubmed-6429194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64291942019-04-15 Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach Santos-Rosales, Víctor Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. Molecules Article Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein:starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO(2) sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications. MDPI 2019-03-01 /pmc/articles/PMC6429194/ /pubmed/30823685 http://dx.doi.org/10.3390/molecules24050871 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Santos-Rosales, Víctor Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach |
title | Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach |
title_full | Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach |
title_fullStr | Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach |
title_full_unstemmed | Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach |
title_short | Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO(2)-Based Approach |
title_sort | sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical co(2)-based approach |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429194/ https://www.ncbi.nlm.nih.gov/pubmed/30823685 http://dx.doi.org/10.3390/molecules24050871 |
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