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Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy

In 2004, we developed autologous periosteal sheets for the treatment of periodontal bone defects. This regenerative therapy has successfully regenerated periodontal bone and augmented alveolar ridge for implant placement. However, the necessity for 6-week culture is a limitation. Here, we examined t...

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Autores principales: Kawase, Tomoyuki, Nagata, Masaki, Okuda, Kazuhiro, Ushiki, Takashi, Fujimoto, Yoko, Watanabe, Mari, Ito, Akira, Nakata, Koh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429500/
https://www.ncbi.nlm.nih.gov/pubmed/30823423
http://dx.doi.org/10.3390/ijms20051053
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author Kawase, Tomoyuki
Nagata, Masaki
Okuda, Kazuhiro
Ushiki, Takashi
Fujimoto, Yoko
Watanabe, Mari
Ito, Akira
Nakata, Koh
author_facet Kawase, Tomoyuki
Nagata, Masaki
Okuda, Kazuhiro
Ushiki, Takashi
Fujimoto, Yoko
Watanabe, Mari
Ito, Akira
Nakata, Koh
author_sort Kawase, Tomoyuki
collection PubMed
description In 2004, we developed autologous periosteal sheets for the treatment of periodontal bone defects. This regenerative therapy has successfully regenerated periodontal bone and augmented alveolar ridge for implant placement. However, the necessity for 6-week culture is a limitation. Here, we examined the applicability of a human platelet-rich fibrin extract (PRFext) as an alternative to fetal bovine serum (FBS) for the explant culture of periosteal sheets in a novel culture medium (MSC-PCM) originally developed for maintaining mesenchymal stem cells. Small periosteum tissue segments were expanded in MSC-PCM + 2% PRFext for 4 weeks, and the resulting periosteal sheets were compared with those prepared by the conventional method using Medium199 + 10% FBS for their growth rate, cell multilayer formation, alkaline phosphatase (ALP) activity, and surface antigen expression (CD73, CD90, and CD105). Periosteal sheets grew faster in the novel culture medium than in the conventional medium. However, assessment of cell shape and ALP activity revealed that the periosteal cells growing in the novel medium were relatively immature. These findings suggest that the novel culture medium featuring PRFext offers advantages by shortening the culture period and excluding possible risks associated with xeno-factors without negatively altering the activity of periosteal sheets.
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spelling pubmed-64295002019-04-10 Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy Kawase, Tomoyuki Nagata, Masaki Okuda, Kazuhiro Ushiki, Takashi Fujimoto, Yoko Watanabe, Mari Ito, Akira Nakata, Koh Int J Mol Sci Article In 2004, we developed autologous periosteal sheets for the treatment of periodontal bone defects. This regenerative therapy has successfully regenerated periodontal bone and augmented alveolar ridge for implant placement. However, the necessity for 6-week culture is a limitation. Here, we examined the applicability of a human platelet-rich fibrin extract (PRFext) as an alternative to fetal bovine serum (FBS) for the explant culture of periosteal sheets in a novel culture medium (MSC-PCM) originally developed for maintaining mesenchymal stem cells. Small periosteum tissue segments were expanded in MSC-PCM + 2% PRFext for 4 weeks, and the resulting periosteal sheets were compared with those prepared by the conventional method using Medium199 + 10% FBS for their growth rate, cell multilayer formation, alkaline phosphatase (ALP) activity, and surface antigen expression (CD73, CD90, and CD105). Periosteal sheets grew faster in the novel culture medium than in the conventional medium. However, assessment of cell shape and ALP activity revealed that the periosteal cells growing in the novel medium were relatively immature. These findings suggest that the novel culture medium featuring PRFext offers advantages by shortening the culture period and excluding possible risks associated with xeno-factors without negatively altering the activity of periosteal sheets. MDPI 2019-02-28 /pmc/articles/PMC6429500/ /pubmed/30823423 http://dx.doi.org/10.3390/ijms20051053 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kawase, Tomoyuki
Nagata, Masaki
Okuda, Kazuhiro
Ushiki, Takashi
Fujimoto, Yoko
Watanabe, Mari
Ito, Akira
Nakata, Koh
Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy
title Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy
title_full Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy
title_fullStr Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy
title_full_unstemmed Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy
title_short Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy
title_sort platelet-rich fibrin extract: a promising fetal bovine serum alternative in explant cultures of human periosteal sheets for regenerative therapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429500/
https://www.ncbi.nlm.nih.gov/pubmed/30823423
http://dx.doi.org/10.3390/ijms20051053
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