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Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum

Red coleoptiles can help crops to cope with adversity and the key genes that are responsible for this trait have previously been isolated from Triticum aestivum, Triticum urartu, and Aegilops tauschii. This report describes the use of transcriptome analysis to determine the candidate gene that contr...

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Autores principales: Cao, Dong, Fan, Jiequn, Xi, Xingyuan, Zong, Yuan, Wang, Dongxia, Zhang, Huaigang, Liu, Baolong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429503/
https://www.ncbi.nlm.nih.gov/pubmed/30866466
http://dx.doi.org/10.3390/molecules24050932
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author Cao, Dong
Fan, Jiequn
Xi, Xingyuan
Zong, Yuan
Wang, Dongxia
Zhang, Huaigang
Liu, Baolong
author_facet Cao, Dong
Fan, Jiequn
Xi, Xingyuan
Zong, Yuan
Wang, Dongxia
Zhang, Huaigang
Liu, Baolong
author_sort Cao, Dong
collection PubMed
description Red coleoptiles can help crops to cope with adversity and the key genes that are responsible for this trait have previously been isolated from Triticum aestivum, Triticum urartu, and Aegilops tauschii. This report describes the use of transcriptome analysis to determine the candidate gene that controls the trait for white coleoptiles in T. monococcum by screening three cultivars with white coleoptiles and two with red coleoptiles. Fifteen structural genes and two transcription factors that are involved in anthocyanin biosynthesis were identified from the assembled UniGene database through BLAST analysis and their transcript levels were then compared in white and red coleoptiles. The majority of the structural genes reflected lower transcript levels in the white than in the red coleoptiles, which implied that transcription factors related to anthocyanin biosynthesis could be candidate genes. The transcript levels of MYC transcription factor TmMYC-A1 were not significantly different between the white and red coleoptiles and all of the TmMYC-A1s contained complete functional domains. The deduced amino acid sequence of the MYB transcription factor TmMYB-A1 in red coleoptiles was homologous to TuMYB-A1, TaMYB-A1, TaMYB-B1, and TaMYB-D1, which control coleoptile color in corresponding species and contained the complete R2R3 MYB domain and the transactivation domain. TmMYB-a1 lost its two functional domains in white coleoptiles due to a single nucleotide deletion that caused premature termination at 13 bp after the initiation codon. Therefore, TmMYB-A1 is likely to be the candidate gene for the control of the red coleoptile trait, and its loss-of-function mutation leads to the white phenotype in T. monococcum.
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spelling pubmed-64295032019-04-15 Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum Cao, Dong Fan, Jiequn Xi, Xingyuan Zong, Yuan Wang, Dongxia Zhang, Huaigang Liu, Baolong Molecules Article Red coleoptiles can help crops to cope with adversity and the key genes that are responsible for this trait have previously been isolated from Triticum aestivum, Triticum urartu, and Aegilops tauschii. This report describes the use of transcriptome analysis to determine the candidate gene that controls the trait for white coleoptiles in T. monococcum by screening three cultivars with white coleoptiles and two with red coleoptiles. Fifteen structural genes and two transcription factors that are involved in anthocyanin biosynthesis were identified from the assembled UniGene database through BLAST analysis and their transcript levels were then compared in white and red coleoptiles. The majority of the structural genes reflected lower transcript levels in the white than in the red coleoptiles, which implied that transcription factors related to anthocyanin biosynthesis could be candidate genes. The transcript levels of MYC transcription factor TmMYC-A1 were not significantly different between the white and red coleoptiles and all of the TmMYC-A1s contained complete functional domains. The deduced amino acid sequence of the MYB transcription factor TmMYB-A1 in red coleoptiles was homologous to TuMYB-A1, TaMYB-A1, TaMYB-B1, and TaMYB-D1, which control coleoptile color in corresponding species and contained the complete R2R3 MYB domain and the transactivation domain. TmMYB-a1 lost its two functional domains in white coleoptiles due to a single nucleotide deletion that caused premature termination at 13 bp after the initiation codon. Therefore, TmMYB-A1 is likely to be the candidate gene for the control of the red coleoptile trait, and its loss-of-function mutation leads to the white phenotype in T. monococcum. MDPI 2019-03-07 /pmc/articles/PMC6429503/ /pubmed/30866466 http://dx.doi.org/10.3390/molecules24050932 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cao, Dong
Fan, Jiequn
Xi, Xingyuan
Zong, Yuan
Wang, Dongxia
Zhang, Huaigang
Liu, Baolong
Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum
title Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum
title_full Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum
title_fullStr Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum
title_full_unstemmed Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum
title_short Transcriptome Analysis Identifies Key Genes Responsible for Red Coleoptiles in Triticum Monococcum
title_sort transcriptome analysis identifies key genes responsible for red coleoptiles in triticum monococcum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429503/
https://www.ncbi.nlm.nih.gov/pubmed/30866466
http://dx.doi.org/10.3390/molecules24050932
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