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Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)

BACKGROUND: Plant growth-promoting rhizobacteria (PGPR) are highly promising biofertilizers that contribute to eco-friendly sustainable agriculture. There have been many reports on the anti-microbial properties of nanoparticles (NPs). Toxic effects of NPs under laboratory conditions have also report...

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Autores principales: Sotoodehnia, Poopak, Mazlan, Norida, Mohd Saud, Halimi, Samsuri, Wahid A., Habib, Sheikh Hasna, Soltangheisi, Amin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431134/
https://www.ncbi.nlm.nih.gov/pubmed/30918747
http://dx.doi.org/10.7717/peerj.6418
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author Sotoodehnia, Poopak
Mazlan, Norida
Mohd Saud, Halimi
Samsuri, Wahid A.
Habib, Sheikh Hasna
Soltangheisi, Amin
author_facet Sotoodehnia, Poopak
Mazlan, Norida
Mohd Saud, Halimi
Samsuri, Wahid A.
Habib, Sheikh Hasna
Soltangheisi, Amin
author_sort Sotoodehnia, Poopak
collection PubMed
description BACKGROUND: Plant growth-promoting rhizobacteria (PGPR) are highly promising biofertilizers that contribute to eco-friendly sustainable agriculture. There have been many reports on the anti-microbial properties of nanoparticles (NPs). Toxic effects of NPs under laboratory conditions have also reported; however, there is a lack of information about their uptake and mobility in organisms under environmental conditions. There is an urgent need to determine the highest concentration of NPs which is not detrimental for growth and proliferation of PGPR. METHODS: Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to measure the size and shape of NPs. Minimum inhibitory concentrations (MIC) of nano-silver on selected beneficial microbes and Ralstonia solanacearum were measured using the microdilution broth method. The percentage of seed germination was measured under in vitro conditions. RESULTS: NPs were spherical with a size of 16 ± 6 nm. Nano-silver at 12–40 mg l(−1) inhibited the growth of bacteria. Seed application at 40 mg l(−1) protected seeds from R. solanacearum and improved the rate of seed germination.
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spelling pubmed-64311342019-03-27 Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus) Sotoodehnia, Poopak Mazlan, Norida Mohd Saud, Halimi Samsuri, Wahid A. Habib, Sheikh Hasna Soltangheisi, Amin PeerJ Agricultural Science BACKGROUND: Plant growth-promoting rhizobacteria (PGPR) are highly promising biofertilizers that contribute to eco-friendly sustainable agriculture. There have been many reports on the anti-microbial properties of nanoparticles (NPs). Toxic effects of NPs under laboratory conditions have also reported; however, there is a lack of information about their uptake and mobility in organisms under environmental conditions. There is an urgent need to determine the highest concentration of NPs which is not detrimental for growth and proliferation of PGPR. METHODS: Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to measure the size and shape of NPs. Minimum inhibitory concentrations (MIC) of nano-silver on selected beneficial microbes and Ralstonia solanacearum were measured using the microdilution broth method. The percentage of seed germination was measured under in vitro conditions. RESULTS: NPs were spherical with a size of 16 ± 6 nm. Nano-silver at 12–40 mg l(−1) inhibited the growth of bacteria. Seed application at 40 mg l(−1) protected seeds from R. solanacearum and improved the rate of seed germination. PeerJ Inc. 2019-03-20 /pmc/articles/PMC6431134/ /pubmed/30918747 http://dx.doi.org/10.7717/peerj.6418 Text en ©2019 Sotoodehnia et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Sotoodehnia, Poopak
Mazlan, Norida
Mohd Saud, Halimi
Samsuri, Wahid A.
Habib, Sheikh Hasna
Soltangheisi, Amin
Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
title Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
title_full Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
title_fullStr Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
title_full_unstemmed Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
title_short Minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on Ralstonia solanacearum and seed germination of Japanese Cucumber (Cucumis sativus)
title_sort minimum inhibitory concentration of nano-silver bactericides for beneficial microbes and its effect on ralstonia solanacearum and seed germination of japanese cucumber (cucumis sativus)
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431134/
https://www.ncbi.nlm.nih.gov/pubmed/30918747
http://dx.doi.org/10.7717/peerj.6418
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