A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation

The development of a reliable electrochemical sensor using a hybrid nanocomposite consisting of ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate) functionalized graphene oxide (GrO-IL) and gold nanoparticles (AuNPs) stabilized by chitosan (Chit) was described. The new sensor, labelled a...

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Detalles Bibliográficos
Autores principales: Shpigun, Liliya K., Andryukhina, Elena Yu.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431463/
https://www.ncbi.nlm.nih.gov/pubmed/30984441
http://dx.doi.org/10.1155/2019/1572526
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author Shpigun, Liliya K.
Andryukhina, Elena Yu.
author_facet Shpigun, Liliya K.
Andryukhina, Elena Yu.
author_sort Shpigun, Liliya K.
collection PubMed
description The development of a reliable electrochemical sensor using a hybrid nanocomposite consisting of ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate) functionalized graphene oxide (GrO-IL) and gold nanoparticles (AuNPs) stabilized by chitosan (Chit) was described. The new sensor, labelled as GrO-IL-AuNPs-Chit/CSE, exhibited an improved electrocatalytic response to cancer drugs such as purine antimetabolites (6-thioguanine, 6-mercaptopurine, and azathioprine) in a wide concentration range with a low detection limit (20–40 nmol·L(−1), S/N = 3), and satisfactory recoveries (97.1–103.0%). The sensor has been also successfully used for cyclic voltammetric study of a salmon sperm double-stranded DNA degradation and DNA-6-mercaptopurine interaction in aqueous solutions (pH 7.4).
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spelling pubmed-64314632019-04-14 A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation Shpigun, Liliya K. Andryukhina, Elena Yu. J Anal Methods Chem Research Article The development of a reliable electrochemical sensor using a hybrid nanocomposite consisting of ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate) functionalized graphene oxide (GrO-IL) and gold nanoparticles (AuNPs) stabilized by chitosan (Chit) was described. The new sensor, labelled as GrO-IL-AuNPs-Chit/CSE, exhibited an improved electrocatalytic response to cancer drugs such as purine antimetabolites (6-thioguanine, 6-mercaptopurine, and azathioprine) in a wide concentration range with a low detection limit (20–40 nmol·L(−1), S/N = 3), and satisfactory recoveries (97.1–103.0%). The sensor has been also successfully used for cyclic voltammetric study of a salmon sperm double-stranded DNA degradation and DNA-6-mercaptopurine interaction in aqueous solutions (pH 7.4). Hindawi 2019-03-07 /pmc/articles/PMC6431463/ /pubmed/30984441 http://dx.doi.org/10.1155/2019/1572526 Text en Copyright © 2019 Liliya K. Shpigun and Elena Yu. Andryukhina. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shpigun, Liliya K.
Andryukhina, Elena Yu.
A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation
title A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation
title_full A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation
title_fullStr A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation
title_full_unstemmed A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation
title_short A New Electrochemical Sensor for Direct Detection of Purine Antimetabolites and DNA Degradation
title_sort new electrochemical sensor for direct detection of purine antimetabolites and dna degradation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431463/
https://www.ncbi.nlm.nih.gov/pubmed/30984441
http://dx.doi.org/10.1155/2019/1572526
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