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Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities
Secondary metabolites of the roots of Eleutherococcus spp. cultivated in Poland, or the bioactivity, are not fully known. The 75% methanol extracts of five Eleutherococcus spp. (E. senticosus, E. divaricatus, E. sessiliflorus, E. gracilistylus, and E. henryi) were examined for the content of polyphe...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431473/ https://www.ncbi.nlm.nih.gov/pubmed/30984341 http://dx.doi.org/10.1155/2019/8673521 |
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author | Adamczyk, Kuba Olech, Marta Abramek, Jagoda Pietrzak, Wioleta Kuźniewski, Rafał Bogucka-Kocka, Anna Nowak, Renata Ptaszyńska, Aneta A. Rapacka-Gackowska, Alina Skalski, Tomasz Strzemski, Maciej Sowa, Ireneusz Wójciak-Kosior, Magdalena Feldo, Marcin Załuski, Daniel |
author_facet | Adamczyk, Kuba Olech, Marta Abramek, Jagoda Pietrzak, Wioleta Kuźniewski, Rafał Bogucka-Kocka, Anna Nowak, Renata Ptaszyńska, Aneta A. Rapacka-Gackowska, Alina Skalski, Tomasz Strzemski, Maciej Sowa, Ireneusz Wójciak-Kosior, Magdalena Feldo, Marcin Załuski, Daniel |
author_sort | Adamczyk, Kuba |
collection | PubMed |
description | Secondary metabolites of the roots of Eleutherococcus spp. cultivated in Poland, or the bioactivity, are not fully known. The 75% methanol extracts of five Eleutherococcus spp. (E. senticosus, E. divaricatus, E. sessiliflorus, E. gracilistylus, and E. henryi) were examined for the content of polyphenols and phenolic acids as well as for antiacetylcholinesterase, antihyaluronidase, anti-DPPH(∗), and cytotoxic activities. The richest in polyphenols were the roots of E. henryi (10.4 mg/g DW), while in flavonoids the roots of E. divaricatus (6.5 mg/g DW). The richest in phenolic acids occurred the roots of E. henryi [protocatechuic acid (1865 μg/g DE), caffeic acid (244 μg/g DE), and p-coumaric and ferulic acids (55 μg/g DE)]. The highest inhibition of AChE was observed for E. gracilistylus and E. sessiliflorus (32%), at the concentration of 100 μg/0.19 mL of the reaction mixture, while that of Hyal for the roots of E. henryi (40.7%), at the concentration of 100 μg/0.16 mL of the reaction mixture. Among five species tested, the E. henryi extract exhibited the strongest HL-60 cell line growth's inhibition (IC(50) 270 μg/mL). The extracts reduced DPPH(∗) in a time-dependent mode, at the concentration of 0.8 mg/mL. After 90 min from 14.7 to 26.2%, DPPH(∗)was reduced. A phytochemical composition and activity of the Eleutherococcus species, cultivated in Poland, are still under research; however, on the basis of the results obtained, it may be concluded that they may become a source of phytochemicals and be useful for Europe's citizens. |
format | Online Article Text |
id | pubmed-6431473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-64314732019-04-14 Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities Adamczyk, Kuba Olech, Marta Abramek, Jagoda Pietrzak, Wioleta Kuźniewski, Rafał Bogucka-Kocka, Anna Nowak, Renata Ptaszyńska, Aneta A. Rapacka-Gackowska, Alina Skalski, Tomasz Strzemski, Maciej Sowa, Ireneusz Wójciak-Kosior, Magdalena Feldo, Marcin Załuski, Daniel Oxid Med Cell Longev Research Article Secondary metabolites of the roots of Eleutherococcus spp. cultivated in Poland, or the bioactivity, are not fully known. The 75% methanol extracts of five Eleutherococcus spp. (E. senticosus, E. divaricatus, E. sessiliflorus, E. gracilistylus, and E. henryi) were examined for the content of polyphenols and phenolic acids as well as for antiacetylcholinesterase, antihyaluronidase, anti-DPPH(∗), and cytotoxic activities. The richest in polyphenols were the roots of E. henryi (10.4 mg/g DW), while in flavonoids the roots of E. divaricatus (6.5 mg/g DW). The richest in phenolic acids occurred the roots of E. henryi [protocatechuic acid (1865 μg/g DE), caffeic acid (244 μg/g DE), and p-coumaric and ferulic acids (55 μg/g DE)]. The highest inhibition of AChE was observed for E. gracilistylus and E. sessiliflorus (32%), at the concentration of 100 μg/0.19 mL of the reaction mixture, while that of Hyal for the roots of E. henryi (40.7%), at the concentration of 100 μg/0.16 mL of the reaction mixture. Among five species tested, the E. henryi extract exhibited the strongest HL-60 cell line growth's inhibition (IC(50) 270 μg/mL). The extracts reduced DPPH(∗) in a time-dependent mode, at the concentration of 0.8 mg/mL. After 90 min from 14.7 to 26.2%, DPPH(∗)was reduced. A phytochemical composition and activity of the Eleutherococcus species, cultivated in Poland, are still under research; however, on the basis of the results obtained, it may be concluded that they may become a source of phytochemicals and be useful for Europe's citizens. Hindawi 2019-03-10 /pmc/articles/PMC6431473/ /pubmed/30984341 http://dx.doi.org/10.1155/2019/8673521 Text en Copyright © 2019 Kuba Adamczyk et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Adamczyk, Kuba Olech, Marta Abramek, Jagoda Pietrzak, Wioleta Kuźniewski, Rafał Bogucka-Kocka, Anna Nowak, Renata Ptaszyńska, Aneta A. Rapacka-Gackowska, Alina Skalski, Tomasz Strzemski, Maciej Sowa, Ireneusz Wójciak-Kosior, Magdalena Feldo, Marcin Załuski, Daniel Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities |
title |
Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities |
title_full |
Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities |
title_fullStr |
Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities |
title_full_unstemmed |
Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities |
title_short |
Eleutherococcus Species Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities |
title_sort | eleutherococcus species cultivated in europe: a new source of compounds with antiacetylcholinesterase, antihyaluronidase, anti-dpph, and cytotoxic activities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431473/ https://www.ncbi.nlm.nih.gov/pubmed/30984341 http://dx.doi.org/10.1155/2019/8673521 |
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