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Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes

Extracts from plastrum testudinis (PTE) are active compounds that have been used to treat bone diseases in traditional Chinese medicine for thousands of years. In previous studies, we demonstrated their effects on glucocorticoid-induced osteoporosis both in vivo and in vitro. However, the mechanisms...

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Autores principales: Shang, Qi, Yu, Xiang, Ren, Hui, Shen, Gengyang, Zhao, Wenhua, Zhang, Zhida, Huang, Jinjing, Yu, Peiyuan, Liang, De, Tang, Jingjing, Jiang, Xiaobing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431499/
https://www.ncbi.nlm.nih.gov/pubmed/30984279
http://dx.doi.org/10.1155/2019/6815620
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author Shang, Qi
Yu, Xiang
Ren, Hui
Shen, Gengyang
Zhao, Wenhua
Zhang, Zhida
Huang, Jinjing
Yu, Peiyuan
Liang, De
Tang, Jingjing
Jiang, Xiaobing
author_facet Shang, Qi
Yu, Xiang
Ren, Hui
Shen, Gengyang
Zhao, Wenhua
Zhang, Zhida
Huang, Jinjing
Yu, Peiyuan
Liang, De
Tang, Jingjing
Jiang, Xiaobing
author_sort Shang, Qi
collection PubMed
description Extracts from plastrum testudinis (PTE) are active compounds that have been used to treat bone diseases in traditional Chinese medicine for thousands of years. In previous studies, we demonstrated their effects on glucocorticoid-induced osteoporosis both in vivo and in vitro. However, the mechanisms by which PTE regulates the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro remain poorly understood. In this study, rBMSCs were treated with medium (CON), PTE, osteogenic induction (OI), and a combination of PTE and OI (PTE+OI) over a 21-day period. We found that PTE significantly promoted rBMSCs osteogenic differentiation and mineralisation after 21 days of culturing. Moreover, PTE+OI further enhanced the differentiation and mineralisation process. PTE upregulated STE20, IGF1R, and p38 MAPK mRNA expression and downregulated TRAF6 mRNA expression. The extracts inhibited TRAF6 protein expression and promoted STE20, IGF1R, and phosphorylated p38 MAPK protein expression. Our results imply that PTE promotes the proliferation and osteogenic differentiation of rBMSCs by upregulating p38 MAPK, STE20, and IGF1R and downregulating TRAF6 expression, which may provide experimental evidence of the potential of PTE in the treatment of osteoporosis.
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spelling pubmed-64314992019-04-14 Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes Shang, Qi Yu, Xiang Ren, Hui Shen, Gengyang Zhao, Wenhua Zhang, Zhida Huang, Jinjing Yu, Peiyuan Liang, De Tang, Jingjing Jiang, Xiaobing Evid Based Complement Alternat Med Research Article Extracts from plastrum testudinis (PTE) are active compounds that have been used to treat bone diseases in traditional Chinese medicine for thousands of years. In previous studies, we demonstrated their effects on glucocorticoid-induced osteoporosis both in vivo and in vitro. However, the mechanisms by which PTE regulates the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) in vitro remain poorly understood. In this study, rBMSCs were treated with medium (CON), PTE, osteogenic induction (OI), and a combination of PTE and OI (PTE+OI) over a 21-day period. We found that PTE significantly promoted rBMSCs osteogenic differentiation and mineralisation after 21 days of culturing. Moreover, PTE+OI further enhanced the differentiation and mineralisation process. PTE upregulated STE20, IGF1R, and p38 MAPK mRNA expression and downregulated TRAF6 mRNA expression. The extracts inhibited TRAF6 protein expression and promoted STE20, IGF1R, and phosphorylated p38 MAPK protein expression. Our results imply that PTE promotes the proliferation and osteogenic differentiation of rBMSCs by upregulating p38 MAPK, STE20, and IGF1R and downregulating TRAF6 expression, which may provide experimental evidence of the potential of PTE in the treatment of osteoporosis. Hindawi 2019-03-07 /pmc/articles/PMC6431499/ /pubmed/30984279 http://dx.doi.org/10.1155/2019/6815620 Text en Copyright © 2019 Qi Shang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shang, Qi
Yu, Xiang
Ren, Hui
Shen, Gengyang
Zhao, Wenhua
Zhang, Zhida
Huang, Jinjing
Yu, Peiyuan
Liang, De
Tang, Jingjing
Jiang, Xiaobing
Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
title Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
title_full Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
title_fullStr Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
title_full_unstemmed Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
title_short Effect of Plastrum Testudinis Extracts on the Proliferation and Osteogenic Differentiation of rBMSCs by Regulating p38 MAPK-Related Genes
title_sort effect of plastrum testudinis extracts on the proliferation and osteogenic differentiation of rbmscs by regulating p38 mapk-related genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431499/
https://www.ncbi.nlm.nih.gov/pubmed/30984279
http://dx.doi.org/10.1155/2019/6815620
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