Tadpole serum activity (Rana catesbeian a) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocytes in albino rats’ epithelial cells induced by neoplasia

AIM: This study was conducted to examine the tadpole’s serum activity (Rana catesbeiana) in caspase-3 as a marker of the role of apoptosis and total cytotoxic T lymphocyte (CTL) in albino rats’ epithelial cells induced by neoplasia. Tadpole serumcontains thyroxine hormone that may cause the metamorphosis process and control cell proliferation. MATERIALS AND METHODS: Male rats were induced by 7,12-dimethylbenz (α)anthracene (DMBA) 20 mg/rats twice every week over 5 weeks to stimulate skin neoplasia. Tadpole serum injected intracutaneously after neoplasia is known. The negative control group (C−) was not exposed to DMBA and tadpole serum, while the positive control group (C+) was exposed to DMBA. Treatment groups (T1, T2, and T3) were exposed DMBA and tadpole serum 100%, 75%, and 25%/rat/day, respectively. Samples of skin organ were be made preparations immunohistochemistry interacted with caspase-3 and CTL antibody as the marker. RESULTS: Based on the result, immunohistochemistry from skin neoplasia and given therapy of tadpole serum show that Treatment 1 was the highest caspase-3 and CTL expression. The result of caspase-3 expression in C−, C+, T1, T2, and T3 was 0.00(c)±0.000, 0.70(bc)±0.141, 2.00(a)±0.283, 1.10(b)±0.424, and 1.15(b)±0.495, respectively. The result of CTL expression in C−, C+, T1, T2, and T3 was 0.10(d)±0.200, 1.00(c)±0.230, 2.10(a)±0.529, 1.70(ab)±0.258, and 1.35(bc)±0.443, respectively. CONCLUSION: It can be concluded from the study that tadpole serum (R. catesbeiana) 100% concentration can increase caspase-3 and total CTL in albino rats’ epithelial cells induced by neoplasia.