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Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases

Polyurethanes (PU) are widely used synthetic polymers. The growing amount of PU used industrially has resulted in a worldwide increase of plastic wastes. The related environmental pollution as well as the limited availability of the raw materials based on petrochemicals requires novel solutions for...

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Autores principales: Schmidt, Juliane, Wei, Ren, Oeser, Thorsten, Dedavid e Silva, Lukas Andre, Breite, Daniel, Schulze, Agnes, Zimmermann, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431909/
https://www.ncbi.nlm.nih.gov/pubmed/30970745
http://dx.doi.org/10.3390/polym9020065
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author Schmidt, Juliane
Wei, Ren
Oeser, Thorsten
Dedavid e Silva, Lukas Andre
Breite, Daniel
Schulze, Agnes
Zimmermann, Wolfgang
author_facet Schmidt, Juliane
Wei, Ren
Oeser, Thorsten
Dedavid e Silva, Lukas Andre
Breite, Daniel
Schulze, Agnes
Zimmermann, Wolfgang
author_sort Schmidt, Juliane
collection PubMed
description Polyurethanes (PU) are widely used synthetic polymers. The growing amount of PU used industrially has resulted in a worldwide increase of plastic wastes. The related environmental pollution as well as the limited availability of the raw materials based on petrochemicals requires novel solutions for their efficient degradation and recycling. The degradation of the polyester PU Impranil DLN by the polyester hydrolases LC cutinase (LCC), TfCut2, Tcur1278 and Tcur0390 was analyzed using a turbidimetric assay. The highest hydrolysis rates were obtained with TfCut2 and Tcur0390. TfCut2 also showed a significantly higher substrate affinity for Impranil DLN than the other three enzymes, indicated by a higher adsorption constant K. Significant weight losses of the solid thermoplastic polyester PU (TPU) Elastollan B85A-10 and C85A-10 were detected as a result of the enzymatic degradation by all four polyester hydrolases. Within a reaction time of 200 h at 70 °C, LCC caused weight losses of up to 4.9% and 4.1% of Elastollan B85A-10 and C85A-10, respectively. Gel permeation chromatography confirmed a preferential degradation of the larger polymer chains. Scanning electron microscopy revealed cracks at the surface of the TPU cubes as a result of enzymatic surface erosion. Analysis by Fourier transform infrared spectroscopy indicated that the observed weight losses were a result of the cleavage of ester bonds of the polyester TPU.
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spelling pubmed-64319092019-04-02 Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases Schmidt, Juliane Wei, Ren Oeser, Thorsten Dedavid e Silva, Lukas Andre Breite, Daniel Schulze, Agnes Zimmermann, Wolfgang Polymers (Basel) Article Polyurethanes (PU) are widely used synthetic polymers. The growing amount of PU used industrially has resulted in a worldwide increase of plastic wastes. The related environmental pollution as well as the limited availability of the raw materials based on petrochemicals requires novel solutions for their efficient degradation and recycling. The degradation of the polyester PU Impranil DLN by the polyester hydrolases LC cutinase (LCC), TfCut2, Tcur1278 and Tcur0390 was analyzed using a turbidimetric assay. The highest hydrolysis rates were obtained with TfCut2 and Tcur0390. TfCut2 also showed a significantly higher substrate affinity for Impranil DLN than the other three enzymes, indicated by a higher adsorption constant K. Significant weight losses of the solid thermoplastic polyester PU (TPU) Elastollan B85A-10 and C85A-10 were detected as a result of the enzymatic degradation by all four polyester hydrolases. Within a reaction time of 200 h at 70 °C, LCC caused weight losses of up to 4.9% and 4.1% of Elastollan B85A-10 and C85A-10, respectively. Gel permeation chromatography confirmed a preferential degradation of the larger polymer chains. Scanning electron microscopy revealed cracks at the surface of the TPU cubes as a result of enzymatic surface erosion. Analysis by Fourier transform infrared spectroscopy indicated that the observed weight losses were a result of the cleavage of ester bonds of the polyester TPU. MDPI 2017-02-16 /pmc/articles/PMC6431909/ /pubmed/30970745 http://dx.doi.org/10.3390/polym9020065 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schmidt, Juliane
Wei, Ren
Oeser, Thorsten
Dedavid e Silva, Lukas Andre
Breite, Daniel
Schulze, Agnes
Zimmermann, Wolfgang
Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases
title Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases
title_full Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases
title_fullStr Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases
title_full_unstemmed Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases
title_short Degradation of Polyester Polyurethane by Bacterial Polyester Hydrolases
title_sort degradation of polyester polyurethane by bacterial polyester hydrolases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431909/
https://www.ncbi.nlm.nih.gov/pubmed/30970745
http://dx.doi.org/10.3390/polym9020065
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