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Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers

The realization of gene therapy relies on the development of delivery vectors with high efficiency and biocompatibility. With a multitude of structures accessible, the core challenge is precisely tuning vector structure to probe and optimize structure–property relationships. Employing a modular stra...

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Detalles Bibliográficos
Autores principales: Brucks, Spencer D., Freyer, Jessica L., Lambert, Tristan H., Campos, Luis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431972/
https://www.ncbi.nlm.nih.gov/pubmed/30970759
http://dx.doi.org/10.3390/polym9030079
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author Brucks, Spencer D.
Freyer, Jessica L.
Lambert, Tristan H.
Campos, Luis M.
author_facet Brucks, Spencer D.
Freyer, Jessica L.
Lambert, Tristan H.
Campos, Luis M.
author_sort Brucks, Spencer D.
collection PubMed
description The realization of gene therapy relies on the development of delivery vectors with high efficiency and biocompatibility. With a multitude of structures accessible, the core challenge is precisely tuning vector structure to probe and optimize structure–property relationships. Employing a modular strategy, two pairs of cationic polymers based on the trisaminocyclopropenium (TAC) ion were synthesized where the substituents differ in the degree of alkyl chain branching. All TAC-based polymers exhibited higher transfection efficiencies than the untreated controls, with variable in vitro toxicities. Considering both cytotoxicity and transfection efficacy, an optimal nonviral vector was identified. Our studies highlight the importance of exercising precise control over polymer structure, both in terms of backbone identity and substituent nature, and the necessity of a robust, modular platform from which to study them.
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spelling pubmed-64319722019-04-02 Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers Brucks, Spencer D. Freyer, Jessica L. Lambert, Tristan H. Campos, Luis M. Polymers (Basel) Communication The realization of gene therapy relies on the development of delivery vectors with high efficiency and biocompatibility. With a multitude of structures accessible, the core challenge is precisely tuning vector structure to probe and optimize structure–property relationships. Employing a modular strategy, two pairs of cationic polymers based on the trisaminocyclopropenium (TAC) ion were synthesized where the substituents differ in the degree of alkyl chain branching. All TAC-based polymers exhibited higher transfection efficiencies than the untreated controls, with variable in vitro toxicities. Considering both cytotoxicity and transfection efficacy, an optimal nonviral vector was identified. Our studies highlight the importance of exercising precise control over polymer structure, both in terms of backbone identity and substituent nature, and the necessity of a robust, modular platform from which to study them. MDPI 2017-02-24 /pmc/articles/PMC6431972/ /pubmed/30970759 http://dx.doi.org/10.3390/polym9030079 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Brucks, Spencer D.
Freyer, Jessica L.
Lambert, Tristan H.
Campos, Luis M.
Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers
title Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers
title_full Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers
title_fullStr Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers
title_full_unstemmed Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers
title_short Influence of Substituent Chain Branching on the Transfection Efficacy of Cyclopropenium-Based Polymers
title_sort influence of substituent chain branching on the transfection efficacy of cyclopropenium-based polymers
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431972/
https://www.ncbi.nlm.nih.gov/pubmed/30970759
http://dx.doi.org/10.3390/polym9030079
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