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Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity

Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The correspo...

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Autores principales: Kovačević, Davor, Pratnekar, Rok, Godič Torkar, Karmen, Salopek, Jasmina, Dražić, Goran, Abram, Anže, Bohinc, Klemen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432465/
https://www.ncbi.nlm.nih.gov/pubmed/30974625
http://dx.doi.org/10.3390/polym8100345
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author Kovačević, Davor
Pratnekar, Rok
Godič Torkar, Karmen
Salopek, Jasmina
Dražić, Goran
Abram, Anže
Bohinc, Klemen
author_facet Kovačević, Davor
Pratnekar, Rok
Godič Torkar, Karmen
Salopek, Jasmina
Dražić, Goran
Abram, Anže
Bohinc, Klemen
author_sort Kovačević, Davor
collection PubMed
description Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The corresponding surface roughness and hydrophobicity were determined by atomic force microscopy and tensiometry. The surface charge was examined by the zeta potential measurements of silica particles covered with polyelectrolyte multilayers, whereby ionic strength and polyelectrolyte concentrations significantly influenced the build-up process. For adhesion experiments, we used the bacterium Pseudomonas aeruginosa. The extent of adhered bacteria on the surface was determined by scanning electron microscopy. The results showed that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, since relatively low differences in surface roughness and hydrophobicity were obtained. In the case of polyelectrolyte multilayers terminating with a positively charged layer, bacterial adhesion was more pronounced than in the case when the polyelectrolyte layer was negatively charged.
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spelling pubmed-64324652019-04-02 Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity Kovačević, Davor Pratnekar, Rok Godič Torkar, Karmen Salopek, Jasmina Dražić, Goran Abram, Anže Bohinc, Klemen Polymers (Basel) Article Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The corresponding surface roughness and hydrophobicity were determined by atomic force microscopy and tensiometry. The surface charge was examined by the zeta potential measurements of silica particles covered with polyelectrolyte multilayers, whereby ionic strength and polyelectrolyte concentrations significantly influenced the build-up process. For adhesion experiments, we used the bacterium Pseudomonas aeruginosa. The extent of adhered bacteria on the surface was determined by scanning electron microscopy. The results showed that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, since relatively low differences in surface roughness and hydrophobicity were obtained. In the case of polyelectrolyte multilayers terminating with a positively charged layer, bacterial adhesion was more pronounced than in the case when the polyelectrolyte layer was negatively charged. MDPI 2016-09-26 /pmc/articles/PMC6432465/ /pubmed/30974625 http://dx.doi.org/10.3390/polym8100345 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kovačević, Davor
Pratnekar, Rok
Godič Torkar, Karmen
Salopek, Jasmina
Dražić, Goran
Abram, Anže
Bohinc, Klemen
Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
title Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
title_full Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
title_fullStr Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
title_full_unstemmed Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
title_short Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
title_sort influence of polyelectrolyte multilayer properties on bacterial adhesion capacity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432465/
https://www.ncbi.nlm.nih.gov/pubmed/30974625
http://dx.doi.org/10.3390/polym8100345
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