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Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity
Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The correspo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432465/ https://www.ncbi.nlm.nih.gov/pubmed/30974625 http://dx.doi.org/10.3390/polym8100345 |
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author | Kovačević, Davor Pratnekar, Rok Godič Torkar, Karmen Salopek, Jasmina Dražić, Goran Abram, Anže Bohinc, Klemen |
author_facet | Kovačević, Davor Pratnekar, Rok Godič Torkar, Karmen Salopek, Jasmina Dražić, Goran Abram, Anže Bohinc, Klemen |
author_sort | Kovačević, Davor |
collection | PubMed |
description | Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The corresponding surface roughness and hydrophobicity were determined by atomic force microscopy and tensiometry. The surface charge was examined by the zeta potential measurements of silica particles covered with polyelectrolyte multilayers, whereby ionic strength and polyelectrolyte concentrations significantly influenced the build-up process. For adhesion experiments, we used the bacterium Pseudomonas aeruginosa. The extent of adhered bacteria on the surface was determined by scanning electron microscopy. The results showed that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, since relatively low differences in surface roughness and hydrophobicity were obtained. In the case of polyelectrolyte multilayers terminating with a positively charged layer, bacterial adhesion was more pronounced than in the case when the polyelectrolyte layer was negatively charged. |
format | Online Article Text |
id | pubmed-6432465 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64324652019-04-02 Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity Kovačević, Davor Pratnekar, Rok Godič Torkar, Karmen Salopek, Jasmina Dražić, Goran Abram, Anže Bohinc, Klemen Polymers (Basel) Article Bacterial adhesion can be controlled by different material surface properties, such as surface charge, on which we concentrate in our study. We use a silica surface on which poly(allylamine hydrochloride)/sodium poly(4-styrenesulfonate) (PAH/PSS) polyelectrolyte multilayers were formed. The corresponding surface roughness and hydrophobicity were determined by atomic force microscopy and tensiometry. The surface charge was examined by the zeta potential measurements of silica particles covered with polyelectrolyte multilayers, whereby ionic strength and polyelectrolyte concentrations significantly influenced the build-up process. For adhesion experiments, we used the bacterium Pseudomonas aeruginosa. The extent of adhered bacteria on the surface was determined by scanning electron microscopy. The results showed that the extent of adhered bacteria mostly depends on the type of terminating polyelectrolyte layer, since relatively low differences in surface roughness and hydrophobicity were obtained. In the case of polyelectrolyte multilayers terminating with a positively charged layer, bacterial adhesion was more pronounced than in the case when the polyelectrolyte layer was negatively charged. MDPI 2016-09-26 /pmc/articles/PMC6432465/ /pubmed/30974625 http://dx.doi.org/10.3390/polym8100345 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kovačević, Davor Pratnekar, Rok Godič Torkar, Karmen Salopek, Jasmina Dražić, Goran Abram, Anže Bohinc, Klemen Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity |
title | Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity |
title_full | Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity |
title_fullStr | Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity |
title_full_unstemmed | Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity |
title_short | Influence of Polyelectrolyte Multilayer Properties on Bacterial Adhesion Capacity |
title_sort | influence of polyelectrolyte multilayer properties on bacterial adhesion capacity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432465/ https://www.ncbi.nlm.nih.gov/pubmed/30974625 http://dx.doi.org/10.3390/polym8100345 |
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