Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines

BACKGROUND: This study aimed to clarify the effect of thoracic drainage fluid (DF) on lung cancer cells in vitro. METHODS: We assessed the influence of DF on the proliferation and migration of lung cancer cells (LTEP-a-2 and A549) using the MTT cell proliferation assay and scratch wound assay. Cell...

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Detalles Bibliográficos
Autores principales: Mao, Yuqiang, Yu, Ying, Han, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433100/
https://www.ncbi.nlm.nih.gov/pubmed/30962714
http://dx.doi.org/10.2147/CMAR.S187019
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author Mao, Yuqiang
Yu, Ying
Han, Yun
author_facet Mao, Yuqiang
Yu, Ying
Han, Yun
author_sort Mao, Yuqiang
collection PubMed
description BACKGROUND: This study aimed to clarify the effect of thoracic drainage fluid (DF) on lung cancer cells in vitro. METHODS: We assessed the influence of DF on the proliferation and migration of lung cancer cells (LTEP-a-2 and A549) using the MTT cell proliferation assay and scratch wound assay. Cell apoptosis was determined by flow cytometric analysis. We also investigated the effect of DF on drug chemosensitivity, assessing viability of LTEP-a-2 and A549 cells. RESULTS: The proliferative rates of cancer cells in the DF-treated group were significantly higher than those of the control group. Similar results were obtained for cell migration of lung cancer cells. Cells in the DF-treated groups showed a lower percentage of apoptosis than those of the control groups. Chemosensitivity of lung cancer cells to doxycycline and cisplatin (DDP) was lowered by DF. CONCLUSION: These findings suggest that DF affects lung cancer cells by promoting proliferation and migration, inhibiting apoptosis, and increasing drug resistance.
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spelling pubmed-64331002019-04-08 Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines Mao, Yuqiang Yu, Ying Han, Yun Cancer Manag Res Original Research BACKGROUND: This study aimed to clarify the effect of thoracic drainage fluid (DF) on lung cancer cells in vitro. METHODS: We assessed the influence of DF on the proliferation and migration of lung cancer cells (LTEP-a-2 and A549) using the MTT cell proliferation assay and scratch wound assay. Cell apoptosis was determined by flow cytometric analysis. We also investigated the effect of DF on drug chemosensitivity, assessing viability of LTEP-a-2 and A549 cells. RESULTS: The proliferative rates of cancer cells in the DF-treated group were significantly higher than those of the control group. Similar results were obtained for cell migration of lung cancer cells. Cells in the DF-treated groups showed a lower percentage of apoptosis than those of the control groups. Chemosensitivity of lung cancer cells to doxycycline and cisplatin (DDP) was lowered by DF. CONCLUSION: These findings suggest that DF affects lung cancer cells by promoting proliferation and migration, inhibiting apoptosis, and increasing drug resistance. Dove Medical Press 2019-03-20 /pmc/articles/PMC6433100/ /pubmed/30962714 http://dx.doi.org/10.2147/CMAR.S187019 Text en © 2019 Mao et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Mao, Yuqiang
Yu, Ying
Han, Yun
Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
title Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
title_full Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
title_fullStr Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
title_full_unstemmed Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
title_short Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
title_sort influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433100/
https://www.ncbi.nlm.nih.gov/pubmed/30962714
http://dx.doi.org/10.2147/CMAR.S187019
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