Cargando…

Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition

Impairment spiral arteries remodelling was considered to be the underlying cause of pathogenesis of pre‐eclampsia (PE). Resveratrol (RE) was reported that it could modulate cellar phenotype to ameliorate diverse human diseases. However, the biological function of RE in PE remains poorly understood....

Descripción completa

Detalles Bibliográficos
Autores principales: Zou, Yanfen, Li, Shuhong, Wu, Dan, Xu, Yetao, Wang, Sailan, Jiang, Ying, Liu, Fang, Jiang, Ziyan, Qu, Hongmei, Yu, Xiang, Wang, Xiaoli, Wang, Yuanli, Sun, Lizhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433653/
https://www.ncbi.nlm.nih.gov/pubmed/30710417
http://dx.doi.org/10.1111/jcmm.14175
_version_ 1783406312797765632
author Zou, Yanfen
Li, Shuhong
Wu, Dan
Xu, Yetao
Wang, Sailan
Jiang, Ying
Liu, Fang
Jiang, Ziyan
Qu, Hongmei
Yu, Xiang
Wang, Xiaoli
Wang, Yuanli
Sun, Lizhou
author_facet Zou, Yanfen
Li, Shuhong
Wu, Dan
Xu, Yetao
Wang, Sailan
Jiang, Ying
Liu, Fang
Jiang, Ziyan
Qu, Hongmei
Yu, Xiang
Wang, Xiaoli
Wang, Yuanli
Sun, Lizhou
author_sort Zou, Yanfen
collection PubMed
description Impairment spiral arteries remodelling was considered to be the underlying cause of pathogenesis of pre‐eclampsia (PE). Resveratrol (RE) was reported that it could modulate cellar phenotype to ameliorate diverse human diseases. However, the biological function of RE in PE remains poorly understood. In this report, we investigated the effect of RE on trophoblast phenotype both in vivo and in vitro. We conducted MTT and transwell assays to explore cell proliferation and invasion events in HTR‐8/SVneo. In mice model, the clinical characteristics of PE were established through the injection of NG‐nitro‐l‐arginine methyl ester (L‐NAME). Furthermore, related experiments were performed to detect cellar phenotype‐associated signalling pathway, including epithelial‐mesenchymal transition (EMT) and Wnt/β‐catenin. Cell assays indicated that RE could increase trophoblasts migration and invasion. In addition, hypertension and proteinuria were markedly ameliorated by RE compared with the controls in PE mice model. Moreover, treatment by RE in trophoblasts or in PE model, we found that RE activated EMT progress through the regulation of E‐cadherin, β‐catenin, N‐cadherin, vimentin expression, and further altered the WNT‐related gene expression, including WNT1, WNT3 and WNT5B. Our findings demonstrated that RE might stimulate the invasive capability of human trophoblasts by promoting EMT and mediating the Wnt/β‐catenin pathway in PE.
format Online
Article
Text
id pubmed-6433653
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-64336532019-04-08 Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition Zou, Yanfen Li, Shuhong Wu, Dan Xu, Yetao Wang, Sailan Jiang, Ying Liu, Fang Jiang, Ziyan Qu, Hongmei Yu, Xiang Wang, Xiaoli Wang, Yuanli Sun, Lizhou J Cell Mol Med Original Articles Impairment spiral arteries remodelling was considered to be the underlying cause of pathogenesis of pre‐eclampsia (PE). Resveratrol (RE) was reported that it could modulate cellar phenotype to ameliorate diverse human diseases. However, the biological function of RE in PE remains poorly understood. In this report, we investigated the effect of RE on trophoblast phenotype both in vivo and in vitro. We conducted MTT and transwell assays to explore cell proliferation and invasion events in HTR‐8/SVneo. In mice model, the clinical characteristics of PE were established through the injection of NG‐nitro‐l‐arginine methyl ester (L‐NAME). Furthermore, related experiments were performed to detect cellar phenotype‐associated signalling pathway, including epithelial‐mesenchymal transition (EMT) and Wnt/β‐catenin. Cell assays indicated that RE could increase trophoblasts migration and invasion. In addition, hypertension and proteinuria were markedly ameliorated by RE compared with the controls in PE mice model. Moreover, treatment by RE in trophoblasts or in PE model, we found that RE activated EMT progress through the regulation of E‐cadherin, β‐catenin, N‐cadherin, vimentin expression, and further altered the WNT‐related gene expression, including WNT1, WNT3 and WNT5B. Our findings demonstrated that RE might stimulate the invasive capability of human trophoblasts by promoting EMT and mediating the Wnt/β‐catenin pathway in PE. John Wiley and Sons Inc. 2019-02-01 2019-04 /pmc/articles/PMC6433653/ /pubmed/30710417 http://dx.doi.org/10.1111/jcmm.14175 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Zou, Yanfen
Li, Shuhong
Wu, Dan
Xu, Yetao
Wang, Sailan
Jiang, Ying
Liu, Fang
Jiang, Ziyan
Qu, Hongmei
Yu, Xiang
Wang, Xiaoli
Wang, Yuanli
Sun, Lizhou
Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
title Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
title_full Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
title_fullStr Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
title_full_unstemmed Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
title_short Resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
title_sort resveratrol promotes trophoblast invasion in pre‐eclampsia by inducing epithelial‐mesenchymal transition
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433653/
https://www.ncbi.nlm.nih.gov/pubmed/30710417
http://dx.doi.org/10.1111/jcmm.14175
work_keys_str_mv AT zouyanfen resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT lishuhong resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT wudan resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT xuyetao resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT wangsailan resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT jiangying resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT liufang resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT jiangziyan resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT quhongmei resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT yuxiang resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT wangxiaoli resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT wangyuanli resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition
AT sunlizhou resveratrolpromotestrophoblastinvasioninpreeclampsiabyinducingepithelialmesenchymaltransition