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Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components

Abscisic acid (ABA) plays an important role in various aspects of plant growth and development, including adaptation to stresses, fruit development and ripening. In seeds, ABA participates through its core signaling components in dormancy instauration, longevity determination, and inhibition of germ...

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Autores principales: Ruggiero, Alessandra, Landi, Simone, Punzo, Paola, Possenti, Marco, Van Oosten, Michael J., Costa, Antonello, Morelli, Giorgio, Maggio, Albino, Grillo, Stefania, Batelli, Giorgia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433719/
https://www.ncbi.nlm.nih.gov/pubmed/30941154
http://dx.doi.org/10.3389/fpls.2019.00304
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author Ruggiero, Alessandra
Landi, Simone
Punzo, Paola
Possenti, Marco
Van Oosten, Michael J.
Costa, Antonello
Morelli, Giorgio
Maggio, Albino
Grillo, Stefania
Batelli, Giorgia
author_facet Ruggiero, Alessandra
Landi, Simone
Punzo, Paola
Possenti, Marco
Van Oosten, Michael J.
Costa, Antonello
Morelli, Giorgio
Maggio, Albino
Grillo, Stefania
Batelli, Giorgia
author_sort Ruggiero, Alessandra
collection PubMed
description Abscisic acid (ABA) plays an important role in various aspects of plant growth and development, including adaptation to stresses, fruit development and ripening. In seeds, ABA participates through its core signaling components in dormancy instauration, longevity determination, and inhibition of germination in unfavorable environmental conditions such as high soil salinity. Here, we show that seed germination in pepper was delayed but only marginally reduced by ABA or NaCl with respect to control treatments. Through a similarity search, pepper orthologs of ABA core signaling components PYL (PYRABACTIN RESISTANCE1-LIKE), PP2C (PROTEIN PHOSPHATASE2C), and SnRK2 (SUCROSE NONFERMENTING1 (SNF1)-RELATED PROTEIN KINASE2) genes were identified. Gene expression analyses of selected members showed a low abundance of PYL and SnRK2 transcripts in dry seeds compared to other tissues, and an up-regulation at high concentrations of ABA and/or NaCl for both positive and negative regulators of ABA signaling. As expected, in hydroponically-grown seedlings exposed to NaCl, only PP2C encoding genes were up-regulated. Yeast two hybrid assays performed among putative pepper core components and with Arabidopsis thaliana orthologs confirmed the ability of the identified proteins to function in ABA signaling cascade, with the exception of a CaABI isoform cloned from seeds. BiFC assay in planta confirmed some of the interactions obtained in yeast. Altogether, our results indicate that a low expression of perception and signaling components in pepper seeds might contribute to explain the observed high percentages of seed germination in the presence of ABA. These results might have direct implications on the improvement of seed longevity and vigor, a bottleneck in pepper breeding.
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spelling pubmed-64337192019-04-02 Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components Ruggiero, Alessandra Landi, Simone Punzo, Paola Possenti, Marco Van Oosten, Michael J. Costa, Antonello Morelli, Giorgio Maggio, Albino Grillo, Stefania Batelli, Giorgia Front Plant Sci Plant Science Abscisic acid (ABA) plays an important role in various aspects of plant growth and development, including adaptation to stresses, fruit development and ripening. In seeds, ABA participates through its core signaling components in dormancy instauration, longevity determination, and inhibition of germination in unfavorable environmental conditions such as high soil salinity. Here, we show that seed germination in pepper was delayed but only marginally reduced by ABA or NaCl with respect to control treatments. Through a similarity search, pepper orthologs of ABA core signaling components PYL (PYRABACTIN RESISTANCE1-LIKE), PP2C (PROTEIN PHOSPHATASE2C), and SnRK2 (SUCROSE NONFERMENTING1 (SNF1)-RELATED PROTEIN KINASE2) genes were identified. Gene expression analyses of selected members showed a low abundance of PYL and SnRK2 transcripts in dry seeds compared to other tissues, and an up-regulation at high concentrations of ABA and/or NaCl for both positive and negative regulators of ABA signaling. As expected, in hydroponically-grown seedlings exposed to NaCl, only PP2C encoding genes were up-regulated. Yeast two hybrid assays performed among putative pepper core components and with Arabidopsis thaliana orthologs confirmed the ability of the identified proteins to function in ABA signaling cascade, with the exception of a CaABI isoform cloned from seeds. BiFC assay in planta confirmed some of the interactions obtained in yeast. Altogether, our results indicate that a low expression of perception and signaling components in pepper seeds might contribute to explain the observed high percentages of seed germination in the presence of ABA. These results might have direct implications on the improvement of seed longevity and vigor, a bottleneck in pepper breeding. Frontiers Media S.A. 2019-03-19 /pmc/articles/PMC6433719/ /pubmed/30941154 http://dx.doi.org/10.3389/fpls.2019.00304 Text en Copyright © 2019 Ruggiero, Landi, Punzo, Possenti, Van Oosten, Costa, Morelli, Maggio, Grillo and Batelli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ruggiero, Alessandra
Landi, Simone
Punzo, Paola
Possenti, Marco
Van Oosten, Michael J.
Costa, Antonello
Morelli, Giorgio
Maggio, Albino
Grillo, Stefania
Batelli, Giorgia
Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components
title Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components
title_full Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components
title_fullStr Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components
title_full_unstemmed Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components
title_short Salinity and ABA Seed Responses in Pepper: Expression and Interaction of ABA Core Signaling Components
title_sort salinity and aba seed responses in pepper: expression and interaction of aba core signaling components
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433719/
https://www.ncbi.nlm.nih.gov/pubmed/30941154
http://dx.doi.org/10.3389/fpls.2019.00304
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