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A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane

TonB-dependent transporters (TBDTs) are ubiquitous outer membrane β-barrel proteins that import nutrients and bacteriocins across the outer membrane in a proton motive force-dependent manner, by directly connecting to the ExbB/ExbD/TonB system in the inner membrane. Here, we show that the TBDT Oar i...

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Autores principales: Gómez-Santos, Nuria, Glatter, Timo, Koebnik, Ralf, Świątek-Połatyńska, Magdalena Anna, Søgaard-Andersen, Lotte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434023/
https://www.ncbi.nlm.nih.gov/pubmed/30911012
http://dx.doi.org/10.1038/s41467-019-09366-9
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author Gómez-Santos, Nuria
Glatter, Timo
Koebnik, Ralf
Świątek-Połatyńska, Magdalena Anna
Søgaard-Andersen, Lotte
author_facet Gómez-Santos, Nuria
Glatter, Timo
Koebnik, Ralf
Świątek-Połatyńska, Magdalena Anna
Søgaard-Andersen, Lotte
author_sort Gómez-Santos, Nuria
collection PubMed
description TonB-dependent transporters (TBDTs) are ubiquitous outer membrane β-barrel proteins that import nutrients and bacteriocins across the outer membrane in a proton motive force-dependent manner, by directly connecting to the ExbB/ExbD/TonB system in the inner membrane. Here, we show that the TBDT Oar in Myxococcus xanthus is required for secretion of a protein, protease PopC, to the extracellular milieu. PopC accumulates in the periplasm before secretion across the outer membrane, and the proton motive force has a role in secretion to the extracellular milieu. Reconstitution experiments in Escherichia coli demonstrate that secretion of PopC across the outer membrane not only depends on Oar but also on the ExbB/ExbD/TonB system. Our results indicate that TBDTs and the ExbB/ExbD/TonB system may have roles not only in import processes but also in secretion of proteins.
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spelling pubmed-64340232019-03-27 A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane Gómez-Santos, Nuria Glatter, Timo Koebnik, Ralf Świątek-Połatyńska, Magdalena Anna Søgaard-Andersen, Lotte Nat Commun Article TonB-dependent transporters (TBDTs) are ubiquitous outer membrane β-barrel proteins that import nutrients and bacteriocins across the outer membrane in a proton motive force-dependent manner, by directly connecting to the ExbB/ExbD/TonB system in the inner membrane. Here, we show that the TBDT Oar in Myxococcus xanthus is required for secretion of a protein, protease PopC, to the extracellular milieu. PopC accumulates in the periplasm before secretion across the outer membrane, and the proton motive force has a role in secretion to the extracellular milieu. Reconstitution experiments in Escherichia coli demonstrate that secretion of PopC across the outer membrane not only depends on Oar but also on the ExbB/ExbD/TonB system. Our results indicate that TBDTs and the ExbB/ExbD/TonB system may have roles not only in import processes but also in secretion of proteins. Nature Publishing Group UK 2019-03-25 /pmc/articles/PMC6434023/ /pubmed/30911012 http://dx.doi.org/10.1038/s41467-019-09366-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gómez-Santos, Nuria
Glatter, Timo
Koebnik, Ralf
Świątek-Połatyńska, Magdalena Anna
Søgaard-Andersen, Lotte
A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
title A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
title_full A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
title_fullStr A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
title_full_unstemmed A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
title_short A TonB-dependent transporter is required for secretion of protease PopC across the bacterial outer membrane
title_sort tonb-dependent transporter is required for secretion of protease popc across the bacterial outer membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434023/
https://www.ncbi.nlm.nih.gov/pubmed/30911012
http://dx.doi.org/10.1038/s41467-019-09366-9
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