Optimizing genome editing strategy by primer-extension-mediated sequencing

Efficient and precise genome editing is essential for clinical applications and generating animal models, which requires engineered nucleases with high editing ability while low off-target activity. Here we present a high-throughput sequencing method, primer-extension-mediated sequencing (PEM-seq),...

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Detalles Bibliográficos
Autores principales: Yin, Jianhang, Liu, Mengzhu, Liu, Yang, Wu, Jinchun, Gan, Tingting, Zhang, Weiwei, Li, Yinghui, Zhou, Yaxuan, Hu, Jiazhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434046/
https://www.ncbi.nlm.nih.gov/pubmed/30937179
http://dx.doi.org/10.1038/s41421-019-0088-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434046/
https://www.ncbi.nlm.nih.gov/pubmed/30937179
http://dx.doi.org/10.1038/s41421-019-0088-8

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