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The influence of a single hemodialysis procedure on human T lymphocytes
At the moment it is unknown to what extent the impaired function of T lymphocytes in ESRD patients depends on uremia, and to what extent on hemodialysis (HD) procedure. Therefore, the purpose of the study was to evaluate percentages of T lymphocyte subpopulations ex vivo, plasma concentrations of IL...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434050/ https://www.ncbi.nlm.nih.gov/pubmed/30911040 http://dx.doi.org/10.1038/s41598-019-41619-x |
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author | Lisowska, Katarzyna A. Pindel, Małgorzata Pietruczuk, Krzysztof Kuźmiuk-Glembin, Izabella Storoniak, Hanna Dębska-Ślizień, Alicja Witkowski, Jacek M. |
author_facet | Lisowska, Katarzyna A. Pindel, Małgorzata Pietruczuk, Krzysztof Kuźmiuk-Glembin, Izabella Storoniak, Hanna Dębska-Ślizień, Alicja Witkowski, Jacek M. |
author_sort | Lisowska, Katarzyna A. |
collection | PubMed |
description | At the moment it is unknown to what extent the impaired function of T lymphocytes in ESRD patients depends on uremia, and to what extent on hemodialysis (HD) procedure. Therefore, the purpose of the study was to evaluate percentages of T lymphocyte subpopulations ex vivo, plasma concentrations of IL12p70, TNF, IL-10, IL-6, IL-1β, IL-8 cytokines and selected proliferation parameters of in vitro activated T lymphocytes in HD patients before and after single HD procedure using flow cytometry. We demonstrated that the percentage of CD8(+) cells ex vivo was decreased while the CD4(+)/CD8(+) ratio was increased after HD procedure. Also, there was significant decrease in the percentage of CD8(+)HLA-DR(+), CD8(+)CD69(+) and CD8(+)CD95(+) cells after HD. At the same time, an increase in the percentage of CD4(+)CD95(+) cells was observed after HD. From all analyzed cytokines, only the concentration of IL-8 was significantly decreased after HD procedure. A single HD session enhanced proliferation capacity of CD4(+) cells but not CD8(+) cells in vitro by increasing number of cell divisions and percentage of dividing cells. Our results show that a single hemodialysis can have immunomodulatory effect on HD patients and may contribute to the state of immune deficiency observed in patients with ESRD. |
format | Online Article Text |
id | pubmed-6434050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64340502019-04-02 The influence of a single hemodialysis procedure on human T lymphocytes Lisowska, Katarzyna A. Pindel, Małgorzata Pietruczuk, Krzysztof Kuźmiuk-Glembin, Izabella Storoniak, Hanna Dębska-Ślizień, Alicja Witkowski, Jacek M. Sci Rep Article At the moment it is unknown to what extent the impaired function of T lymphocytes in ESRD patients depends on uremia, and to what extent on hemodialysis (HD) procedure. Therefore, the purpose of the study was to evaluate percentages of T lymphocyte subpopulations ex vivo, plasma concentrations of IL12p70, TNF, IL-10, IL-6, IL-1β, IL-8 cytokines and selected proliferation parameters of in vitro activated T lymphocytes in HD patients before and after single HD procedure using flow cytometry. We demonstrated that the percentage of CD8(+) cells ex vivo was decreased while the CD4(+)/CD8(+) ratio was increased after HD procedure. Also, there was significant decrease in the percentage of CD8(+)HLA-DR(+), CD8(+)CD69(+) and CD8(+)CD95(+) cells after HD. At the same time, an increase in the percentage of CD4(+)CD95(+) cells was observed after HD. From all analyzed cytokines, only the concentration of IL-8 was significantly decreased after HD procedure. A single HD session enhanced proliferation capacity of CD4(+) cells but not CD8(+) cells in vitro by increasing number of cell divisions and percentage of dividing cells. Our results show that a single hemodialysis can have immunomodulatory effect on HD patients and may contribute to the state of immune deficiency observed in patients with ESRD. Nature Publishing Group UK 2019-03-25 /pmc/articles/PMC6434050/ /pubmed/30911040 http://dx.doi.org/10.1038/s41598-019-41619-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Lisowska, Katarzyna A. Pindel, Małgorzata Pietruczuk, Krzysztof Kuźmiuk-Glembin, Izabella Storoniak, Hanna Dębska-Ślizień, Alicja Witkowski, Jacek M. The influence of a single hemodialysis procedure on human T lymphocytes |
title | The influence of a single hemodialysis procedure on human T lymphocytes |
title_full | The influence of a single hemodialysis procedure on human T lymphocytes |
title_fullStr | The influence of a single hemodialysis procedure on human T lymphocytes |
title_full_unstemmed | The influence of a single hemodialysis procedure on human T lymphocytes |
title_short | The influence of a single hemodialysis procedure on human T lymphocytes |
title_sort | influence of a single hemodialysis procedure on human t lymphocytes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434050/ https://www.ncbi.nlm.nih.gov/pubmed/30911040 http://dx.doi.org/10.1038/s41598-019-41619-x |
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