Cargando…

miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A

We investigated the effect of miR-182-5p on the viability, proliferation, invasion, and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma ti...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yuling, Chen, Shudong, Shan, Zhengfei, Bi, Liyan, Yu, Shengqiang, Li, Yongwei, Xu, Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434084/
https://www.ncbi.nlm.nih.gov/pubmed/28546229
http://dx.doi.org/10.1042/BSR20170136
_version_ 1783406410415996928
author Li, Yuling
Chen, Shudong
Shan, Zhengfei
Bi, Liyan
Yu, Shengqiang
Li, Yongwei
Xu, Sen
author_facet Li, Yuling
Chen, Shudong
Shan, Zhengfei
Bi, Liyan
Yu, Shengqiang
Li, Yongwei
Xu, Sen
author_sort Li, Yuling
collection PubMed
description We investigated the effect of miR-182-5p on the viability, proliferation, invasion, and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma tissues, and different cell lines. Western blotting was also used to determine the RAB27A expression in both tissues and cell lines. We chose the HGC-27 cell line as experiment subject as it demonstrated the highest miR-182-5p level. HGC-27 cells were transfected with different vectors and the cell viability, mitosis, invasion, and migration ability were measured through MTT assay, flow cytometry (FCM) analysis, Transwell assay, and wound healing assay. In comparison with the normal tissues, miR-182-5p is expressed at a higher level in gastric cancer (GC) tissues, while RAB27A is expressed at a lower level in cancerous tissues. The down-regulation of miR-182-5p and up-regulation of RAB27A can significantly decrease the viability, migration, invasion, and mitosis of HGC-27 cells. The target relationship between miR-182-5p and RAb27A was confirmed through a dual-luciferase reporter gene assay and Western blot assay. miR-182-5p enhances the viability, mitosis, migration, and invasion of human GC cells by down-regulating RAB27A.
format Online
Article
Text
id pubmed-6434084
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-64340842019-04-12 miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A Li, Yuling Chen, Shudong Shan, Zhengfei Bi, Liyan Yu, Shengqiang Li, Yongwei Xu, Sen Biosci Rep Research Articles We investigated the effect of miR-182-5p on the viability, proliferation, invasion, and migration ability of human gastric cells by regulating the expression of RAB27A. Real-time PCR assay was used to detect the expression of miR-182-5 and RAB27A in human gastric carcinoma tissues, para-carcinoma tissues, and different cell lines. Western blotting was also used to determine the RAB27A expression in both tissues and cell lines. We chose the HGC-27 cell line as experiment subject as it demonstrated the highest miR-182-5p level. HGC-27 cells were transfected with different vectors and the cell viability, mitosis, invasion, and migration ability were measured through MTT assay, flow cytometry (FCM) analysis, Transwell assay, and wound healing assay. In comparison with the normal tissues, miR-182-5p is expressed at a higher level in gastric cancer (GC) tissues, while RAB27A is expressed at a lower level in cancerous tissues. The down-regulation of miR-182-5p and up-regulation of RAB27A can significantly decrease the viability, migration, invasion, and mitosis of HGC-27 cells. The target relationship between miR-182-5p and RAb27A was confirmed through a dual-luciferase reporter gene assay and Western blot assay. miR-182-5p enhances the viability, mitosis, migration, and invasion of human GC cells by down-regulating RAB27A. Portland Press Ltd. 2017-06-27 /pmc/articles/PMC6434084/ /pubmed/28546229 http://dx.doi.org/10.1042/BSR20170136 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Li, Yuling
Chen, Shudong
Shan, Zhengfei
Bi, Liyan
Yu, Shengqiang
Li, Yongwei
Xu, Sen
miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
title miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
title_full miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
title_fullStr miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
title_full_unstemmed miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
title_short miR-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating RAB27A
title_sort mir-182-5p improves the viability, mitosis, migration, and invasion ability of human gastric cancer cells by down-regulating rab27a
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434084/
https://www.ncbi.nlm.nih.gov/pubmed/28546229
http://dx.doi.org/10.1042/BSR20170136
work_keys_str_mv AT liyuling mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a
AT chenshudong mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a
AT shanzhengfei mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a
AT biliyan mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a
AT yushengqiang mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a
AT liyongwei mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a
AT xusen mir1825pimprovestheviabilitymitosismigrationandinvasionabilityofhumangastriccancercellsbydownregulatingrab27a