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Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434243/ https://www.ncbi.nlm.nih.gov/pubmed/30936956 http://dx.doi.org/10.3892/etm.2019.7261 |
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author | Zheng, Yan Yang, Yao Wang, Jiaman Liang, Yanhua |
author_facet | Zheng, Yan Yang, Yao Wang, Jiaman Liang, Yanhua |
author_sort | Zheng, Yan |
collection | PubMed |
description | Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN) has been identified as a commonly overexpressed proto-oncogene in several types of visceral cancer. However, to the best of our knowledge, the functions of SHARPIN in nonmelanoma skin cancer (NMSC) have not been described. The present study aimed to investigate the expression of SHARPIN protein and SHARPIN mutations in NMSC. A total of 85 BCC, 77 SCC and 21 keratoacanthoma (KA) formalin-fixed paraffin-embedded (FFPE) samples were collected. SHARPIN expression was detected using immunohistochemistry. DNA was extracted from the FFPE samples, and the sequences of SHARPIN were analyzed using polymerase chain reaction. In addition, high and moderate expression levels of SHARPIN were observed in normal skin tissues and KA samples. However, the expression of SHARPIN was absent in cancer nests and was significantly low in precancerous NMSC lesions. The total mutation frequency of SHARPIN was 21.8% in BCC and 17.0% in SCC. These data indicate that SHARPIN may serve a tumor-suppressing role and be a promising diagnostic, prognostic and therapeutic biomarker in NMSC. |
format | Online Article Text |
id | pubmed-6434243 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-64342432019-04-01 Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer Zheng, Yan Yang, Yao Wang, Jiaman Liang, Yanhua Exp Ther Med Articles Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN) has been identified as a commonly overexpressed proto-oncogene in several types of visceral cancer. However, to the best of our knowledge, the functions of SHARPIN in nonmelanoma skin cancer (NMSC) have not been described. The present study aimed to investigate the expression of SHARPIN protein and SHARPIN mutations in NMSC. A total of 85 BCC, 77 SCC and 21 keratoacanthoma (KA) formalin-fixed paraffin-embedded (FFPE) samples were collected. SHARPIN expression was detected using immunohistochemistry. DNA was extracted from the FFPE samples, and the sequences of SHARPIN were analyzed using polymerase chain reaction. In addition, high and moderate expression levels of SHARPIN were observed in normal skin tissues and KA samples. However, the expression of SHARPIN was absent in cancer nests and was significantly low in precancerous NMSC lesions. The total mutation frequency of SHARPIN was 21.8% in BCC and 17.0% in SCC. These data indicate that SHARPIN may serve a tumor-suppressing role and be a promising diagnostic, prognostic and therapeutic biomarker in NMSC. D.A. Spandidos 2019-04 2019-02-12 /pmc/articles/PMC6434243/ /pubmed/30936956 http://dx.doi.org/10.3892/etm.2019.7261 Text en Copyright: © Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zheng, Yan Yang, Yao Wang, Jiaman Liang, Yanhua Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer |
title | Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer |
title_full | Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer |
title_fullStr | Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer |
title_full_unstemmed | Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer |
title_short | Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer |
title_sort | aberrant expression and high-frequency mutations of sharpin in nonmelanoma skin cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434243/ https://www.ncbi.nlm.nih.gov/pubmed/30936956 http://dx.doi.org/10.3892/etm.2019.7261 |
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