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Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer

Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN)...

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Autores principales: Zheng, Yan, Yang, Yao, Wang, Jiaman, Liang, Yanhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434243/
https://www.ncbi.nlm.nih.gov/pubmed/30936956
http://dx.doi.org/10.3892/etm.2019.7261
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author Zheng, Yan
Yang, Yao
Wang, Jiaman
Liang, Yanhua
author_facet Zheng, Yan
Yang, Yao
Wang, Jiaman
Liang, Yanhua
author_sort Zheng, Yan
collection PubMed
description Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN) has been identified as a commonly overexpressed proto-oncogene in several types of visceral cancer. However, to the best of our knowledge, the functions of SHARPIN in nonmelanoma skin cancer (NMSC) have not been described. The present study aimed to investigate the expression of SHARPIN protein and SHARPIN mutations in NMSC. A total of 85 BCC, 77 SCC and 21 keratoacanthoma (KA) formalin-fixed paraffin-embedded (FFPE) samples were collected. SHARPIN expression was detected using immunohistochemistry. DNA was extracted from the FFPE samples, and the sequences of SHARPIN were analyzed using polymerase chain reaction. In addition, high and moderate expression levels of SHARPIN were observed in normal skin tissues and KA samples. However, the expression of SHARPIN was absent in cancer nests and was significantly low in precancerous NMSC lesions. The total mutation frequency of SHARPIN was 21.8% in BCC and 17.0% in SCC. These data indicate that SHARPIN may serve a tumor-suppressing role and be a promising diagnostic, prognostic and therapeutic biomarker in NMSC.
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spelling pubmed-64342432019-04-01 Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer Zheng, Yan Yang, Yao Wang, Jiaman Liang, Yanhua Exp Ther Med Articles Squamous cell carcinoma (SCC) and basal cell carcinoma (BCC) have exhibited a marked increase in incidence in previous decades and are the most common malignancies in Caucasian populations. Src homology 3 and multiple ankyrin repeat domains protein-associated RH domain-interacting protein (SHARPIN) has been identified as a commonly overexpressed proto-oncogene in several types of visceral cancer. However, to the best of our knowledge, the functions of SHARPIN in nonmelanoma skin cancer (NMSC) have not been described. The present study aimed to investigate the expression of SHARPIN protein and SHARPIN mutations in NMSC. A total of 85 BCC, 77 SCC and 21 keratoacanthoma (KA) formalin-fixed paraffin-embedded (FFPE) samples were collected. SHARPIN expression was detected using immunohistochemistry. DNA was extracted from the FFPE samples, and the sequences of SHARPIN were analyzed using polymerase chain reaction. In addition, high and moderate expression levels of SHARPIN were observed in normal skin tissues and KA samples. However, the expression of SHARPIN was absent in cancer nests and was significantly low in precancerous NMSC lesions. The total mutation frequency of SHARPIN was 21.8% in BCC and 17.0% in SCC. These data indicate that SHARPIN may serve a tumor-suppressing role and be a promising diagnostic, prognostic and therapeutic biomarker in NMSC. D.A. Spandidos 2019-04 2019-02-12 /pmc/articles/PMC6434243/ /pubmed/30936956 http://dx.doi.org/10.3892/etm.2019.7261 Text en Copyright: © Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zheng, Yan
Yang, Yao
Wang, Jiaman
Liang, Yanhua
Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
title Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
title_full Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
title_fullStr Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
title_full_unstemmed Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
title_short Aberrant expression and high-frequency mutations of SHARPIN in nonmelanoma skin cancer
title_sort aberrant expression and high-frequency mutations of sharpin in nonmelanoma skin cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434243/
https://www.ncbi.nlm.nih.gov/pubmed/30936956
http://dx.doi.org/10.3892/etm.2019.7261
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