Cargando…

The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo

Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results fro...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimomura, Tadahiro, Kawakami, Masayoshi, Tatsumi, Kouko, Tanaka, Tatsuhide, Morita-Takemura, Shoko, Kirita, Tadaaki, Wanaka, Akio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434314/
https://www.ncbi.nlm.nih.gov/pubmed/30923412
http://dx.doi.org/10.1267/ahc.18038
_version_ 1783406457373327360
author Shimomura, Tadahiro
Kawakami, Masayoshi
Tatsumi, Kouko
Tanaka, Tatsuhide
Morita-Takemura, Shoko
Kirita, Tadaaki
Wanaka, Akio
author_facet Shimomura, Tadahiro
Kawakami, Masayoshi
Tatsumi, Kouko
Tanaka, Tatsuhide
Morita-Takemura, Shoko
Kirita, Tadaaki
Wanaka, Akio
author_sort Shimomura, Tadahiro
collection PubMed
description Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results from the failure of the Wnt signaling pathway. To examine whether Wnt signaling can influences upper jaw development, we applied beads soaked with Dickkopf-1 (Dkk-1), Alsterpaullone (AL) or Wnt3a to the right side of the maxillary prominence of the chick embryo. The embryo showed a defect of the maxilla on the treated side, and skeletal staining revealed hypoplasia of the premaxilla and palatine bone as a result of Dkk-1-soaked bead implantation. 5-bromo-2'-deoxyuridine (BrdU)-positive cell numbers in the treated maxillary prominence were significantly lower at both 24 and 48 hr after implantation. Down-regulation of the expression of Bmp4, Tbx22, Sox9, and Barx1 was confirmed in the maxillary prominence treated with Dkk-1, which indicated that the deformity of the maxillary bone was controlled by gene targets of the Wnt signaling pathway. Expression of N-cadherin was seen immunohistochemically in the maxillary prominences of embryos at 6 hr and increased at 24 hr after AL treatment. Wnt signaling enhanced by AL or Wnt3a up-regulated the expression levels of Msx1, Bmp4, Tbx22, Sox9, and Barx1. Our data suggest that the Wnt signaling pathway regulates maxillary morphogenesis and growth through Bmp4, Tbx22, Sox9, and Barx1. Wnt signaling might regulate N-cadherin expression via Msx1, resulting in cell aggregation for osteochondrogenesis.
format Online
Article
Text
id pubmed-6434314
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
record_format MEDLINE/PubMed
spelling pubmed-64343142019-03-28 The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo Shimomura, Tadahiro Kawakami, Masayoshi Tatsumi, Kouko Tanaka, Tatsuhide Morita-Takemura, Shoko Kirita, Tadaaki Wanaka, Akio Acta Histochem Cytochem Regular Article Cleft lip with or without cleft palate (CLP) usually results from a failure of the medial nasal prominences to fuse with the lateral and maxillary prominences. This failure inhibits facial morphogenesis regulated by several major morphogenetic signaling pathways. We hypothesized that CLP results from the failure of the Wnt signaling pathway. To examine whether Wnt signaling can influences upper jaw development, we applied beads soaked with Dickkopf-1 (Dkk-1), Alsterpaullone (AL) or Wnt3a to the right side of the maxillary prominence of the chick embryo. The embryo showed a defect of the maxilla on the treated side, and skeletal staining revealed hypoplasia of the premaxilla and palatine bone as a result of Dkk-1-soaked bead implantation. 5-bromo-2'-deoxyuridine (BrdU)-positive cell numbers in the treated maxillary prominence were significantly lower at both 24 and 48 hr after implantation. Down-regulation of the expression of Bmp4, Tbx22, Sox9, and Barx1 was confirmed in the maxillary prominence treated with Dkk-1, which indicated that the deformity of the maxillary bone was controlled by gene targets of the Wnt signaling pathway. Expression of N-cadherin was seen immunohistochemically in the maxillary prominences of embryos at 6 hr and increased at 24 hr after AL treatment. Wnt signaling enhanced by AL or Wnt3a up-regulated the expression levels of Msx1, Bmp4, Tbx22, Sox9, and Barx1. Our data suggest that the Wnt signaling pathway regulates maxillary morphogenesis and growth through Bmp4, Tbx22, Sox9, and Barx1. Wnt signaling might regulate N-cadherin expression via Msx1, resulting in cell aggregation for osteochondrogenesis. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2019-02-28 2019-02-23 /pmc/articles/PMC6434314/ /pubmed/30923412 http://dx.doi.org/10.1267/ahc.18038 Text en 2019 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Shimomura, Tadahiro
Kawakami, Masayoshi
Tatsumi, Kouko
Tanaka, Tatsuhide
Morita-Takemura, Shoko
Kirita, Tadaaki
Wanaka, Akio
The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo
title The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo
title_full The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo
title_fullStr The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo
title_full_unstemmed The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo
title_short The Role of the Wnt Signaling Pathway in Upper Jaw Development of Chick Embryo
title_sort role of the wnt signaling pathway in upper jaw development of chick embryo
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434314/
https://www.ncbi.nlm.nih.gov/pubmed/30923412
http://dx.doi.org/10.1267/ahc.18038
work_keys_str_mv AT shimomuratadahiro theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT kawakamimasayoshi theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT tatsumikouko theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT tanakatatsuhide theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT moritatakemurashoko theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT kiritatadaaki theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT wanakaakio theroleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT shimomuratadahiro roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT kawakamimasayoshi roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT tatsumikouko roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT tanakatatsuhide roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT moritatakemurashoko roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT kiritatadaaki roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo
AT wanakaakio roleofthewntsignalingpathwayinupperjawdevelopmentofchickembryo