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EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma
Epidermal growth factor receptor variant III (EGFRvIII) is a tumor-specific cell surface antigen often expressed in glioblastoma and has drawn much attention as a possible therapeutic target. We performed immunohistochemistry on histology sections of surgical specimens taken from 67 cases with gliob...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japan Neurosurgical Society
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434422/ https://www.ncbi.nlm.nih.gov/pubmed/30787232 http://dx.doi.org/10.2176/nmc.oa.2018-0078 |
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author | NOZAWA, Takanori OKADA, Masayasu NATSUMEDA, Manabu EDA, Takeyoshi ABE, Hideaki TSUKAMOTO, Yoshihiro OKAMOTO, Kouichirou OISHI, Makoto TAKAHASHI, Hitoshi FUJII, Yukihiko KAKITA, Akiyoshi |
author_facet | NOZAWA, Takanori OKADA, Masayasu NATSUMEDA, Manabu EDA, Takeyoshi ABE, Hideaki TSUKAMOTO, Yoshihiro OKAMOTO, Kouichirou OISHI, Makoto TAKAHASHI, Hitoshi FUJII, Yukihiko KAKITA, Akiyoshi |
author_sort | NOZAWA, Takanori |
collection | PubMed |
description | Epidermal growth factor receptor variant III (EGFRvIII) is a tumor-specific cell surface antigen often expressed in glioblastoma and has drawn much attention as a possible therapeutic target. We performed immunohistochemistry on histology sections of surgical specimens taken from 67 cases with glioblastoma, isocitrate dehydrogenase-wild type, and evaluated the morphological characteristics and distribution of the EGFRvIII-positive tumor cells. We then evaluated the localization of EGFRvIII-expression within the tumor and peritumoral areas. EGFRvIII immunopositivity was detected in 15 specimens taken from 13 patients, including two recurrent specimens taken from the same patient at relapse. Immunofluorescence staining demonstrated that EGFRvIII-positive cells were present in cells positive for glial fibrillary acidic protein (GFAP), and some showed astrocytic differentiation with multiple fine processes and others did not shown. The EGFRvIII-positive cells were located in cellular areas of the tumor, but not in the invading zone. In the two recurrent cases, EGFRvIII-positive cells were markedly decreased in one case and retained in the other. With regard to overall survival, univariate analysis indicated that EGFRvIII-expression in patients with glioblastoma was not significantly associated with a favorable outcome. Double-labeling immunofluorescence staining of EGFRvIII and GFAP showed that processes of large, well differentiated, GFAP-positive glia extend to and surround less differentiated, EGFRvIII-positive glial cells in cellular areas of tumor. However, in the tumor periphery, EGFRvIII-positive tumor cells were not observed. This finding suggests that EGFRvIII is involved in tumor proliferation, but that invading glioma cells lose their EGFRvIII expression. |
format | Online Article Text |
id | pubmed-6434422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Japan Neurosurgical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-64344222019-03-27 EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma NOZAWA, Takanori OKADA, Masayasu NATSUMEDA, Manabu EDA, Takeyoshi ABE, Hideaki TSUKAMOTO, Yoshihiro OKAMOTO, Kouichirou OISHI, Makoto TAKAHASHI, Hitoshi FUJII, Yukihiko KAKITA, Akiyoshi Neurol Med Chir (Tokyo) Original Article Epidermal growth factor receptor variant III (EGFRvIII) is a tumor-specific cell surface antigen often expressed in glioblastoma and has drawn much attention as a possible therapeutic target. We performed immunohistochemistry on histology sections of surgical specimens taken from 67 cases with glioblastoma, isocitrate dehydrogenase-wild type, and evaluated the morphological characteristics and distribution of the EGFRvIII-positive tumor cells. We then evaluated the localization of EGFRvIII-expression within the tumor and peritumoral areas. EGFRvIII immunopositivity was detected in 15 specimens taken from 13 patients, including two recurrent specimens taken from the same patient at relapse. Immunofluorescence staining demonstrated that EGFRvIII-positive cells were present in cells positive for glial fibrillary acidic protein (GFAP), and some showed astrocytic differentiation with multiple fine processes and others did not shown. The EGFRvIII-positive cells were located in cellular areas of the tumor, but not in the invading zone. In the two recurrent cases, EGFRvIII-positive cells were markedly decreased in one case and retained in the other. With regard to overall survival, univariate analysis indicated that EGFRvIII-expression in patients with glioblastoma was not significantly associated with a favorable outcome. Double-labeling immunofluorescence staining of EGFRvIII and GFAP showed that processes of large, well differentiated, GFAP-positive glia extend to and surround less differentiated, EGFRvIII-positive glial cells in cellular areas of tumor. However, in the tumor periphery, EGFRvIII-positive tumor cells were not observed. This finding suggests that EGFRvIII is involved in tumor proliferation, but that invading glioma cells lose their EGFRvIII expression. The Japan Neurosurgical Society 2019-03 2019-02-21 /pmc/articles/PMC6434422/ /pubmed/30787232 http://dx.doi.org/10.2176/nmc.oa.2018-0078 Text en © 2019 The Japan Neurosurgical Society This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Original Article NOZAWA, Takanori OKADA, Masayasu NATSUMEDA, Manabu EDA, Takeyoshi ABE, Hideaki TSUKAMOTO, Yoshihiro OKAMOTO, Kouichirou OISHI, Makoto TAKAHASHI, Hitoshi FUJII, Yukihiko KAKITA, Akiyoshi EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma |
title | EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma |
title_full | EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma |
title_fullStr | EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma |
title_full_unstemmed | EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma |
title_short | EGFRvIII Is Expressed in Cellular Areas of Tumor in a Subset of Glioblastoma |
title_sort | egfrviii is expressed in cellular areas of tumor in a subset of glioblastoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434422/ https://www.ncbi.nlm.nih.gov/pubmed/30787232 http://dx.doi.org/10.2176/nmc.oa.2018-0078 |
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