Characteristics of Female Germline Stem Cells from Porcine Ovaries at Sexual Maturity

Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexua...

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Detalles Bibliográficos
Autores principales: Hou, Lin, Wang, Jian, Li, Xinyue, Wang, Hu, Liu, Guishu, Xu, Bo, Mei, Xingxing, Hua, Xiuguo, Wu, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434470/
https://www.ncbi.nlm.nih.gov/pubmed/29991280
http://dx.doi.org/10.1177/0963689718784878
Descripción
Sumario:Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexual maturity. Here, we isolated, purified, and cultured FGSCs from porcine ovaries at sexual maturity. Furthermore, we established and characterized the porcine FGSC (pFGSC) lines. In addition, we found that pFGSC lines could differentiate into oocytes when injection into tissue grafts, including human ovarian tissues. The results show that FGSCs exist in ovaries of Banna mini-pigs at juvenile and sexually maturity. These findings have implications in animal biotechnology applications and regeneration medicine.

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