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Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier

Following the tremendous development of hydrogels for cell therapy, there is now a growing need for surgical techniques to validate in vivo scaffold benefits for islet transplantation. Therefore, we propose a newly designed surgical procedure involving the injection of hydrogel-embedded pancreatic i...

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Autores principales: Schaschkow, Anaïs, Sigrist, Séverine, Mura, Carole, Dissaux, Caroline, Bouzakri, Karim, Lejay, Anne, Bruant-Rodier, Catherine, Pinget, Michel, Maillard, Elisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434471/
https://www.ncbi.nlm.nih.gov/pubmed/29996661
http://dx.doi.org/10.1177/0963689718784873
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author Schaschkow, Anaïs
Sigrist, Séverine
Mura, Carole
Dissaux, Caroline
Bouzakri, Karim
Lejay, Anne
Bruant-Rodier, Catherine
Pinget, Michel
Maillard, Elisa
author_facet Schaschkow, Anaïs
Sigrist, Séverine
Mura, Carole
Dissaux, Caroline
Bouzakri, Karim
Lejay, Anne
Bruant-Rodier, Catherine
Pinget, Michel
Maillard, Elisa
author_sort Schaschkow, Anaïs
collection PubMed
description Following the tremendous development of hydrogels for cell therapy, there is now a growing need for surgical techniques to validate in vivo scaffold benefits for islet transplantation. Therefore, we propose a newly designed surgical procedure involving the injection of hydrogel-embedded pancreatic islets in the omentum, which is considered a favorable environment for cell survival and function. Our technique, called h-Omental Matrix Islet filliNG (hOMING) was designed to test the benefits of hydrogel on islet survival and function in vivo. Islets were implanted in the omentum of diabetic rats using the hOMING technique and alginate as an islet carrier. Blood glucose and C-peptide levels were recorded to assess graft function. After 2 months, grafts were explanted and studied using insulin and vessel staining. All rats that underwent hOMING exhibited graft function characterized by a glycemia decrease and a C-peptidemia increase (P < 0.001 compared with preoperative levels). Furthermore, hOMING appeared to preserve islet morphology and insulin content and allowed the proper revascularization of grafted islets. The results suggest that hOMING is a viable and promising approach to test in vivo the benefits of hydrogel administration for islet transplantation into the omental tissue.
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spelling pubmed-64344712019-04-01 Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier Schaschkow, Anaïs Sigrist, Séverine Mura, Carole Dissaux, Caroline Bouzakri, Karim Lejay, Anne Bruant-Rodier, Catherine Pinget, Michel Maillard, Elisa Cell Transplant Brief Communication Following the tremendous development of hydrogels for cell therapy, there is now a growing need for surgical techniques to validate in vivo scaffold benefits for islet transplantation. Therefore, we propose a newly designed surgical procedure involving the injection of hydrogel-embedded pancreatic islets in the omentum, which is considered a favorable environment for cell survival and function. Our technique, called h-Omental Matrix Islet filliNG (hOMING) was designed to test the benefits of hydrogel on islet survival and function in vivo. Islets were implanted in the omentum of diabetic rats using the hOMING technique and alginate as an islet carrier. Blood glucose and C-peptide levels were recorded to assess graft function. After 2 months, grafts were explanted and studied using insulin and vessel staining. All rats that underwent hOMING exhibited graft function characterized by a glycemia decrease and a C-peptidemia increase (P < 0.001 compared with preoperative levels). Furthermore, hOMING appeared to preserve islet morphology and insulin content and allowed the proper revascularization of grafted islets. The results suggest that hOMING is a viable and promising approach to test in vivo the benefits of hydrogel administration for islet transplantation into the omental tissue. SAGE Publications 2018-07-11 2018-08 /pmc/articles/PMC6434471/ /pubmed/29996661 http://dx.doi.org/10.1177/0963689718784873 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Brief Communication
Schaschkow, Anaïs
Sigrist, Séverine
Mura, Carole
Dissaux, Caroline
Bouzakri, Karim
Lejay, Anne
Bruant-Rodier, Catherine
Pinget, Michel
Maillard, Elisa
Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier
title Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier
title_full Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier
title_fullStr Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier
title_full_unstemmed Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier
title_short Extra-Hepatic Islet Transplantation: Validation of the h-Omental Matrix Islet filliNG (hOMING) Technique on a Rodent Model Using an Alginate Carrier
title_sort extra-hepatic islet transplantation: validation of the h-omental matrix islet filling (homing) technique on a rodent model using an alginate carrier
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434471/
https://www.ncbi.nlm.nih.gov/pubmed/29996661
http://dx.doi.org/10.1177/0963689718784873
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