Intergenerational paternal effect of adult density in Drosophila melanogaster

1. Notwithstanding recent evidences, paternal environment is thought to be a potential but unlikely source of fitness variation that can affect trait evolution. Here we studied intergenerational effects of males’ exposure to varying adult density in Drosophila melanogasterlaboratory populations. 2. We held sires at normal (N), medium (M) and high (H) adult densities for 2 days before allowing them to mate with virgin females. This treatment did not introduce selection through differential mortality. Further, we randomly paired males and females and allowed a single round of mating between the sires and the dams. We then collected eggs from the dams and measured the egg size. Finally, we investigated the effect of the paternal treatment on juvenile and adult (male) fitness components. 3. We found a significant treatment effect on juvenile competitive ability where the progeny sired by the H‐males had higher competitive ability. Since we did not find the treatment to affect egg size, this effect is unlikely to be mediated through variation in female provisioning. 4. Male fitness components were also found to have a significant treatment effect: M‐sons had lower dry weight at eclosion, higher mating latency, and lower competitive mating success. 5. While being the first study to show both adaptive and non‐adaptive effect of the paternal density in Drosophila, our results highlight the importance of considering paternal environment as important source of fitness variation.