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The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect

BACKGROUND: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. AIMS: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on...

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Autores principales: Susanto, Agus, Susanah, Susi, Priosoeryanto, Bambang Pontjo, Satari, Mieke Hemiawati, Komara, Ira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434731/
https://www.ncbi.nlm.nih.gov/pubmed/30983781
http://dx.doi.org/10.4103/jisp.jisp_232_18
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author Susanto, Agus
Susanah, Susi
Priosoeryanto, Bambang Pontjo
Satari, Mieke Hemiawati
Komara, Ira
author_facet Susanto, Agus
Susanah, Susi
Priosoeryanto, Bambang Pontjo
Satari, Mieke Hemiawati
Komara, Ira
author_sort Susanto, Agus
collection PubMed
description BACKGROUND: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. AIMS: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on wound healing in rat mandibular defect by counting the number of fibroblasts and new blood vessels. MATERIALS AND METHODS: As much as 24 male Wistar rats were divided into two groups, the treatment and control group. Bone defects were made In the rat mandible with diamond bur with a diameter of 2 mm, then the defect was covered with a chitosan-collagen membrane, and the control group was covered without application of chitosan-collagen membrane. After the 3(rd), 7(th), 14(th), and the 21(st) day, the defect site was analyzed histologically. The number of fibroblasts and blood vessels was counted under a light microscope, at five fields with ×1000 and ×400 microscope magnification. STATISTICAL ANALYSIS USED: This study was done by using analysis of variance and unpaired t-test. RESULTS: The average number of fibroblasts and blood vessels in the treatment group was higher than the control group. There was a significant difference in the number of fibroblasts on the 3(rd) and 7(th) day (P = 0.001; P = 0.001) and the number of blood vessels on the 3(rd) day (P = 0.04). CONCLUSION: The chitosan-collagen membrane was able to increase the number of fibroblasts and new blood vessels in the wound healing process.
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spelling pubmed-64347312019-04-12 The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect Susanto, Agus Susanah, Susi Priosoeryanto, Bambang Pontjo Satari, Mieke Hemiawati Komara, Ira J Indian Soc Periodontol Original Article BACKGROUND: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. AIMS: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on wound healing in rat mandibular defect by counting the number of fibroblasts and new blood vessels. MATERIALS AND METHODS: As much as 24 male Wistar rats were divided into two groups, the treatment and control group. Bone defects were made In the rat mandible with diamond bur with a diameter of 2 mm, then the defect was covered with a chitosan-collagen membrane, and the control group was covered without application of chitosan-collagen membrane. After the 3(rd), 7(th), 14(th), and the 21(st) day, the defect site was analyzed histologically. The number of fibroblasts and blood vessels was counted under a light microscope, at five fields with ×1000 and ×400 microscope magnification. STATISTICAL ANALYSIS USED: This study was done by using analysis of variance and unpaired t-test. RESULTS: The average number of fibroblasts and blood vessels in the treatment group was higher than the control group. There was a significant difference in the number of fibroblasts on the 3(rd) and 7(th) day (P = 0.001; P = 0.001) and the number of blood vessels on the 3(rd) day (P = 0.04). CONCLUSION: The chitosan-collagen membrane was able to increase the number of fibroblasts and new blood vessels in the wound healing process. Wolters Kluwer - Medknow 2019 /pmc/articles/PMC6434731/ /pubmed/30983781 http://dx.doi.org/10.4103/jisp.jisp_232_18 Text en Copyright: © 2018 Indian Society of Periodontology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Susanto, Agus
Susanah, Susi
Priosoeryanto, Bambang Pontjo
Satari, Mieke Hemiawati
Komara, Ira
The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
title The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
title_full The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
title_fullStr The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
title_full_unstemmed The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
title_short The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
title_sort effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434731/
https://www.ncbi.nlm.nih.gov/pubmed/30983781
http://dx.doi.org/10.4103/jisp.jisp_232_18
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