Cargando…
The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect
BACKGROUND: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. AIMS: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434731/ https://www.ncbi.nlm.nih.gov/pubmed/30983781 http://dx.doi.org/10.4103/jisp.jisp_232_18 |
_version_ | 1783406526907547648 |
---|---|
author | Susanto, Agus Susanah, Susi Priosoeryanto, Bambang Pontjo Satari, Mieke Hemiawati Komara, Ira |
author_facet | Susanto, Agus Susanah, Susi Priosoeryanto, Bambang Pontjo Satari, Mieke Hemiawati Komara, Ira |
author_sort | Susanto, Agus |
collection | PubMed |
description | BACKGROUND: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. AIMS: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on wound healing in rat mandibular defect by counting the number of fibroblasts and new blood vessels. MATERIALS AND METHODS: As much as 24 male Wistar rats were divided into two groups, the treatment and control group. Bone defects were made In the rat mandible with diamond bur with a diameter of 2 mm, then the defect was covered with a chitosan-collagen membrane, and the control group was covered without application of chitosan-collagen membrane. After the 3(rd), 7(th), 14(th), and the 21(st) day, the defect site was analyzed histologically. The number of fibroblasts and blood vessels was counted under a light microscope, at five fields with ×1000 and ×400 microscope magnification. STATISTICAL ANALYSIS USED: This study was done by using analysis of variance and unpaired t-test. RESULTS: The average number of fibroblasts and blood vessels in the treatment group was higher than the control group. There was a significant difference in the number of fibroblasts on the 3(rd) and 7(th) day (P = 0.001; P = 0.001) and the number of blood vessels on the 3(rd) day (P = 0.04). CONCLUSION: The chitosan-collagen membrane was able to increase the number of fibroblasts and new blood vessels in the wound healing process. |
format | Online Article Text |
id | pubmed-6434731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-64347312019-04-12 The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect Susanto, Agus Susanah, Susi Priosoeryanto, Bambang Pontjo Satari, Mieke Hemiawati Komara, Ira J Indian Soc Periodontol Original Article BACKGROUND: Collagen and chitosan are potential biomaterials for medical applications; chitosan-collagen membranes are used as a barrier membrane in guided tissue regeneration and guided bone regeneration. AIMS: The purpose of this study was to analyze the effect of the chitosan-collagen membrane on wound healing in rat mandibular defect by counting the number of fibroblasts and new blood vessels. MATERIALS AND METHODS: As much as 24 male Wistar rats were divided into two groups, the treatment and control group. Bone defects were made In the rat mandible with diamond bur with a diameter of 2 mm, then the defect was covered with a chitosan-collagen membrane, and the control group was covered without application of chitosan-collagen membrane. After the 3(rd), 7(th), 14(th), and the 21(st) day, the defect site was analyzed histologically. The number of fibroblasts and blood vessels was counted under a light microscope, at five fields with ×1000 and ×400 microscope magnification. STATISTICAL ANALYSIS USED: This study was done by using analysis of variance and unpaired t-test. RESULTS: The average number of fibroblasts and blood vessels in the treatment group was higher than the control group. There was a significant difference in the number of fibroblasts on the 3(rd) and 7(th) day (P = 0.001; P = 0.001) and the number of blood vessels on the 3(rd) day (P = 0.04). CONCLUSION: The chitosan-collagen membrane was able to increase the number of fibroblasts and new blood vessels in the wound healing process. Wolters Kluwer - Medknow 2019 /pmc/articles/PMC6434731/ /pubmed/30983781 http://dx.doi.org/10.4103/jisp.jisp_232_18 Text en Copyright: © 2018 Indian Society of Periodontology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Susanto, Agus Susanah, Susi Priosoeryanto, Bambang Pontjo Satari, Mieke Hemiawati Komara, Ira The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
title | The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
title_full | The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
title_fullStr | The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
title_full_unstemmed | The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
title_short | The effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
title_sort | effect of the chitosan-collagen membrane on wound healing process in rat mandibular defect |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434731/ https://www.ncbi.nlm.nih.gov/pubmed/30983781 http://dx.doi.org/10.4103/jisp.jisp_232_18 |
work_keys_str_mv | AT susantoagus theeffectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT susanahsusi theeffectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT priosoeryantobambangpontjo theeffectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT satarimiekehemiawati theeffectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT komaraira theeffectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT susantoagus effectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT susanahsusi effectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT priosoeryantobambangpontjo effectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT satarimiekehemiawati effectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect AT komaraira effectofthechitosancollagenmembraneonwoundhealingprocessinratmandibulardefect |