Cargando…

Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces

BACKGROUND: Stripe rust is a serious fungal disease of wheat (Triticum aestivum L.) caused by Puccinia striiformis f. sp. tritici (Pst), which results in yield reduction and decreased grain quality. Breeding for genetic resistance to stripe rust is the most cost-effective method to control the disea...

Descripción completa

Detalles Bibliográficos
Autores principales: Yao, Fangjie, Zhang, Xuemei, Ye, Xueling, Li, Jian, Long, Li, Yu, Can, Li, Jing, Wang, Yuqi, Wu, Yu, Wang, Jirui, Jiang, Qiantao, Li, Wei, Ma, Jian, Wei, Yuming, Zheng, Youliang, Chen, Guoyue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434810/
https://www.ncbi.nlm.nih.gov/pubmed/30914040
http://dx.doi.org/10.1186/s12863-019-0736-x
_version_ 1783406544949346304
author Yao, Fangjie
Zhang, Xuemei
Ye, Xueling
Li, Jian
Long, Li
Yu, Can
Li, Jing
Wang, Yuqi
Wu, Yu
Wang, Jirui
Jiang, Qiantao
Li, Wei
Ma, Jian
Wei, Yuming
Zheng, Youliang
Chen, Guoyue
author_facet Yao, Fangjie
Zhang, Xuemei
Ye, Xueling
Li, Jian
Long, Li
Yu, Can
Li, Jing
Wang, Yuqi
Wu, Yu
Wang, Jirui
Jiang, Qiantao
Li, Wei
Ma, Jian
Wei, Yuming
Zheng, Youliang
Chen, Guoyue
author_sort Yao, Fangjie
collection PubMed
description BACKGROUND: Stripe rust is a serious fungal disease of wheat (Triticum aestivum L.) caused by Puccinia striiformis f. sp. tritici (Pst), which results in yield reduction and decreased grain quality. Breeding for genetic resistance to stripe rust is the most cost-effective method to control the disease. In the present study, a genome-wide association study (GWAS) was conducted to identify markers linked to stripe rust resistance genes (or loci) in 93 Northern Chinese wheat landraces, using Diversity Arrays Technology (DArT) and simple sequence repeat (SSR) molecular marker technology based on phenotypic data from two field locations over two growing seasons in China. RESULTS: Seventeen accessions were verified to display stable and high levels of adult plant resistance (APR) to stripe rust via multi-environment field assessments. Significant correlations among environments and high heritability were observed for stripe rust infection type (IT) and disease severity (DS). Using mixed linear models (MLM) for the GWAS, a total of 32 significantly associated loci (P < 0.001) were detected. In combination with the linkage disequilibrium (LD) decay distance (6.4 cM), 25 quantitative trait loci (QTL) were identified. Based on the integrated map of previously reported genes and QTL, six QTL located on chromosomes 4A, 6A and 7D were mapped far from resistance regions identified previously, and represent potentially novel stripe rust resistance loci at the adult plant stage. CONCLUSIONS: The present findings demonstrated that identification of genes or loci linked to significant markers in wheat by GWAS is feasible. Seventeen elite accessions conferred with stable and high resistance to stripe rust, and six putative newly detected APR loci were identified among the 93 Northern Chinese wheat landraces. The results illustrate the potential for acceleration of molecular breeding of wheat, and also provide novel sources of stripe rust resistance with potential utility in the breeding of improved wheat cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12863-019-0736-x) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6434810
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-64348102019-04-08 Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces Yao, Fangjie Zhang, Xuemei Ye, Xueling Li, Jian Long, Li Yu, Can Li, Jing Wang, Yuqi Wu, Yu Wang, Jirui Jiang, Qiantao Li, Wei Ma, Jian Wei, Yuming Zheng, Youliang Chen, Guoyue BMC Genet Research Article BACKGROUND: Stripe rust is a serious fungal disease of wheat (Triticum aestivum L.) caused by Puccinia striiformis f. sp. tritici (Pst), which results in yield reduction and decreased grain quality. Breeding for genetic resistance to stripe rust is the most cost-effective method to control the disease. In the present study, a genome-wide association study (GWAS) was conducted to identify markers linked to stripe rust resistance genes (or loci) in 93 Northern Chinese wheat landraces, using Diversity Arrays Technology (DArT) and simple sequence repeat (SSR) molecular marker technology based on phenotypic data from two field locations over two growing seasons in China. RESULTS: Seventeen accessions were verified to display stable and high levels of adult plant resistance (APR) to stripe rust via multi-environment field assessments. Significant correlations among environments and high heritability were observed for stripe rust infection type (IT) and disease severity (DS). Using mixed linear models (MLM) for the GWAS, a total of 32 significantly associated loci (P < 0.001) were detected. In combination with the linkage disequilibrium (LD) decay distance (6.4 cM), 25 quantitative trait loci (QTL) were identified. Based on the integrated map of previously reported genes and QTL, six QTL located on chromosomes 4A, 6A and 7D were mapped far from resistance regions identified previously, and represent potentially novel stripe rust resistance loci at the adult plant stage. CONCLUSIONS: The present findings demonstrated that identification of genes or loci linked to significant markers in wheat by GWAS is feasible. Seventeen elite accessions conferred with stable and high resistance to stripe rust, and six putative newly detected APR loci were identified among the 93 Northern Chinese wheat landraces. The results illustrate the potential for acceleration of molecular breeding of wheat, and also provide novel sources of stripe rust resistance with potential utility in the breeding of improved wheat cultivars. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12863-019-0736-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-26 /pmc/articles/PMC6434810/ /pubmed/30914040 http://dx.doi.org/10.1186/s12863-019-0736-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yao, Fangjie
Zhang, Xuemei
Ye, Xueling
Li, Jian
Long, Li
Yu, Can
Li, Jing
Wang, Yuqi
Wu, Yu
Wang, Jirui
Jiang, Qiantao
Li, Wei
Ma, Jian
Wei, Yuming
Zheng, Youliang
Chen, Guoyue
Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces
title Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces
title_full Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces
title_fullStr Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces
title_full_unstemmed Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces
title_short Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces
title_sort characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in northern chinese wheat landraces
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434810/
https://www.ncbi.nlm.nih.gov/pubmed/30914040
http://dx.doi.org/10.1186/s12863-019-0736-x
work_keys_str_mv AT yaofangjie characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT zhangxuemei characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT yexueling characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT lijian characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT longli characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT yucan characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT lijing characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT wangyuqi characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT wuyu characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT wangjirui characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT jiangqiantao characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT liwei characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT majian characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT weiyuming characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT zhengyouliang characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces
AT chenguoyue characterizationofmoleculardiversityandgenomewideassociationstudyofstriperustresistanceattheadultplantstageinnorthernchinesewheatlandraces