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Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium

BACKGROUND: The synovial membrane adjacent to the articular cartilage is home to synovial mesenchymal progenitor cell (sMPC) populations that have the ability to undergo chondrogenesis. While it has been hypothesized that multiple subtypes of stem and progenitor cells exist in vivo, there is little...

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Autores principales: Affan, Asmaa, Al-Jezani, Nedaa, Railton, Pamela, Powell, James N., Krawetz, Roman J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434889/
https://www.ncbi.nlm.nih.gov/pubmed/30909916
http://dx.doi.org/10.1186/s12891-019-2495-2
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author Affan, Asmaa
Al-Jezani, Nedaa
Railton, Pamela
Powell, James N.
Krawetz, Roman J.
author_facet Affan, Asmaa
Al-Jezani, Nedaa
Railton, Pamela
Powell, James N.
Krawetz, Roman J.
author_sort Affan, Asmaa
collection PubMed
description BACKGROUND: The synovial membrane adjacent to the articular cartilage is home to synovial mesenchymal progenitor cell (sMPC) populations that have the ability to undergo chondrogenesis. While it has been hypothesized that multiple subtypes of stem and progenitor cells exist in vivo, there is little evidence supporting this hypothesis in human tissues. Furthermore, in most of the published literature on this topic, the cells are cultured before derivation of clonal populations. This gap in the literature makes it difficult to determine if there are distinct MPC subtypes in human synovial tissues, and if so, if these sMPCs express any markers in vivo/in situ that provide information in regards to the function of specific MPC subtypes (e.g. cells with increased chondrogenic capacity)? Therefore, the current study was undertaken to determine if any of the classical MPC cell surface markers provide insight into the differentiation capacity of sMPCs. METHODS: Clonal populations of sMPCs were derived from a cohort of patients with hip osteoarthritis (OA) and patients at high risk to develop OA using indexed cell sorting. Tri-differentiation potential and cell surface receptor expression of the resultant clones was determined. RESULTS: A number of clones with distinct differentiation potential were derived from this cohort, yet the most common cell surface marker profile on MPCs (in situ) that demonstrated chondrogenic potential was determined to be CD90(+)/CD44(+)/CD73(+). A validation cohort was employed to isolate cells with only this cell surface profile. Isolating cells directly from human synovial tissue with these three markers alone, did not enrich for cells with chondrogenic capacity. CONCLUSIONS: Therefore, additional markers are required to further discriminate the heterogeneous subtypes of MPCs and identify sMPCs with functional properties that are believed to be advantageous for clinical application. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12891-019-2495-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-64348892019-04-08 Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium Affan, Asmaa Al-Jezani, Nedaa Railton, Pamela Powell, James N. Krawetz, Roman J. BMC Musculoskelet Disord Research Article BACKGROUND: The synovial membrane adjacent to the articular cartilage is home to synovial mesenchymal progenitor cell (sMPC) populations that have the ability to undergo chondrogenesis. While it has been hypothesized that multiple subtypes of stem and progenitor cells exist in vivo, there is little evidence supporting this hypothesis in human tissues. Furthermore, in most of the published literature on this topic, the cells are cultured before derivation of clonal populations. This gap in the literature makes it difficult to determine if there are distinct MPC subtypes in human synovial tissues, and if so, if these sMPCs express any markers in vivo/in situ that provide information in regards to the function of specific MPC subtypes (e.g. cells with increased chondrogenic capacity)? Therefore, the current study was undertaken to determine if any of the classical MPC cell surface markers provide insight into the differentiation capacity of sMPCs. METHODS: Clonal populations of sMPCs were derived from a cohort of patients with hip osteoarthritis (OA) and patients at high risk to develop OA using indexed cell sorting. Tri-differentiation potential and cell surface receptor expression of the resultant clones was determined. RESULTS: A number of clones with distinct differentiation potential were derived from this cohort, yet the most common cell surface marker profile on MPCs (in situ) that demonstrated chondrogenic potential was determined to be CD90(+)/CD44(+)/CD73(+). A validation cohort was employed to isolate cells with only this cell surface profile. Isolating cells directly from human synovial tissue with these three markers alone, did not enrich for cells with chondrogenic capacity. CONCLUSIONS: Therefore, additional markers are required to further discriminate the heterogeneous subtypes of MPCs and identify sMPCs with functional properties that are believed to be advantageous for clinical application. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12891-019-2495-2) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-25 /pmc/articles/PMC6434889/ /pubmed/30909916 http://dx.doi.org/10.1186/s12891-019-2495-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Affan, Asmaa
Al-Jezani, Nedaa
Railton, Pamela
Powell, James N.
Krawetz, Roman J.
Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
title Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
title_full Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
title_fullStr Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
title_full_unstemmed Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
title_short Multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
title_sort multiple mesenchymal progenitor cell subtypes with distinct functional potential are present within the intimal layer of the hip synovium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434889/
https://www.ncbi.nlm.nih.gov/pubmed/30909916
http://dx.doi.org/10.1186/s12891-019-2495-2
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