Cargando…

Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy

OBJECTIVE: Heavy ions have contributed to tumor site-specific radiotherapy and are a major health risk for astronauts. The purpose of this study was to investigate the changes in gene expression in peripheral lymphocytes of cancer patients and astronauts exposed to (12)C ions, and identify suitable...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ruifeng, Dang, Xuhong, Zhang, Zhongxin, Yuan, Yayi, Ren, Yue, Duan, Zhikai, Zuo, Yahui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434914/
https://www.ncbi.nlm.nih.gov/pubmed/30962723
http://dx.doi.org/10.2147/CMAR.S188959
_version_ 1783406569246949376
author Zhang, Ruifeng
Dang, Xuhong
Zhang, Zhongxin
Yuan, Yayi
Ren, Yue
Duan, Zhikai
Zuo, Yahui
author_facet Zhang, Ruifeng
Dang, Xuhong
Zhang, Zhongxin
Yuan, Yayi
Ren, Yue
Duan, Zhikai
Zuo, Yahui
author_sort Zhang, Ruifeng
collection PubMed
description OBJECTIVE: Heavy ions have contributed to tumor site-specific radiotherapy and are a major health risk for astronauts. The purpose of this study was to investigate the changes in gene expression in peripheral lymphocytes of cancer patients and astronauts exposed to (12)C ions, and identify suitable molecular biomarkers for health monitoring. We also aimed to observe the effects of treatment and the level of damage, by comparing the transcriptional profiles of human lymphocyte cell lines exposed to (12)C ion beams at doses of 0–2.0 Gy. MATERIALS AND METHODS: A human lymphocyte cell line was irradiated with (12)C ion beams at 0, 0.1, 0.5, and 2.0 Gy and transcriptional profiles were evaluated using the Agilent human gene expression microarray at 24 hours after irradiation. Differentially expressed genes were identified using a fold change of ≥2.0. Representative genes were further validated by RT-PCR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to determine the roles of differentially expressed mRNAs. RESULTS: Based on the microarray assays, 1,113 genes were upregulated and 853 genes were downregulated in human lymphocyte cells irradiated with 0.1 Gy (12)C ion beams compared with the control group, 1,095 genes were upregulated and 1,220 genes were downregulated in cells irradiated with 0.5 Gy (12)C ion beams, and 1,055 genes were upregulated and 1,356 genes were downregulated in cells irradiated with 2.0 Gy. A total of 504 genes were differentially expressed in all irradiated groups, of which 88 genes were upregulated and 416 genes downregulated. Most of these altered genes were related to the cell cycle, apoptosis, signal transduction, DNA transcription, repair, and replication. The expression differences were further confirmed by RT-PCR for a subset of differentially expressed genes. CONCLUSION: Differentially expressed genes between treatment and control groups at 24 hours post-irradiation increased as the radiation dose increased; upregulated genes gradually decreased and downregulated genes increased. Our data indicated that (12)C ion beams could repress a number of genes in a dose-dependent manner, which might lead to the failure of multiple cellular biological functions.
format Online
Article
Text
id pubmed-6434914
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-64349142019-04-08 Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy Zhang, Ruifeng Dang, Xuhong Zhang, Zhongxin Yuan, Yayi Ren, Yue Duan, Zhikai Zuo, Yahui Cancer Manag Res Original Research OBJECTIVE: Heavy ions have contributed to tumor site-specific radiotherapy and are a major health risk for astronauts. The purpose of this study was to investigate the changes in gene expression in peripheral lymphocytes of cancer patients and astronauts exposed to (12)C ions, and identify suitable molecular biomarkers for health monitoring. We also aimed to observe the effects of treatment and the level of damage, by comparing the transcriptional profiles of human lymphocyte cell lines exposed to (12)C ion beams at doses of 0–2.0 Gy. MATERIALS AND METHODS: A human lymphocyte cell line was irradiated with (12)C ion beams at 0, 0.1, 0.5, and 2.0 Gy and transcriptional profiles were evaluated using the Agilent human gene expression microarray at 24 hours after irradiation. Differentially expressed genes were identified using a fold change of ≥2.0. Representative genes were further validated by RT-PCR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were performed to determine the roles of differentially expressed mRNAs. RESULTS: Based on the microarray assays, 1,113 genes were upregulated and 853 genes were downregulated in human lymphocyte cells irradiated with 0.1 Gy (12)C ion beams compared with the control group, 1,095 genes were upregulated and 1,220 genes were downregulated in cells irradiated with 0.5 Gy (12)C ion beams, and 1,055 genes were upregulated and 1,356 genes were downregulated in cells irradiated with 2.0 Gy. A total of 504 genes were differentially expressed in all irradiated groups, of which 88 genes were upregulated and 416 genes downregulated. Most of these altered genes were related to the cell cycle, apoptosis, signal transduction, DNA transcription, repair, and replication. The expression differences were further confirmed by RT-PCR for a subset of differentially expressed genes. CONCLUSION: Differentially expressed genes between treatment and control groups at 24 hours post-irradiation increased as the radiation dose increased; upregulated genes gradually decreased and downregulated genes increased. Our data indicated that (12)C ion beams could repress a number of genes in a dose-dependent manner, which might lead to the failure of multiple cellular biological functions. Dove Medical Press 2019-03-22 /pmc/articles/PMC6434914/ /pubmed/30962723 http://dx.doi.org/10.2147/CMAR.S188959 Text en © 2019 Zhang et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Zhang, Ruifeng
Dang, Xuhong
Zhang, Zhongxin
Yuan, Yayi
Ren, Yue
Duan, Zhikai
Zuo, Yahui
Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy
title Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy
title_full Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy
title_fullStr Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy
title_full_unstemmed Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy
title_short Comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)C ion beams at 0–2.0 Gy
title_sort comparison of transcriptional profiles in human lymphocyte cells irradiated with (12)c ion beams at 0–2.0 gy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434914/
https://www.ncbi.nlm.nih.gov/pubmed/30962723
http://dx.doi.org/10.2147/CMAR.S188959
work_keys_str_mv AT zhangruifeng comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy
AT dangxuhong comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy
AT zhangzhongxin comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy
AT yuanyayi comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy
AT renyue comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy
AT duanzhikai comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy
AT zuoyahui comparisonoftranscriptionalprofilesinhumanlymphocytecellsirradiatedwith12cionbeamsat020gy