Cargando…

miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation

MicroRNAs (miRNAs) play an essential role in articular cartilage development and growth. However, the exact mechanisms involved in this process remain unknown. In the present study, we investigated the biological functions of miR-27b during hypertrophic differentiation of rat articular chondrocytes....

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Jinying, Lv, Shuang, Hou, Yi, Xu, Kan, Sun, Dongjie, Zheng, Yangyang, Zhang, Zechuan, Li, Xianglan, Li, Yulin, Chi, Guangfan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435457/
https://www.ncbi.nlm.nih.gov/pubmed/29187585
http://dx.doi.org/10.1042/BSR20171109
_version_ 1783406634422239232
author Xu, Jinying
Lv, Shuang
Hou, Yi
Xu, Kan
Sun, Dongjie
Zheng, Yangyang
Zhang, Zechuan
Li, Xianglan
Li, Yulin
Chi, Guangfan
author_facet Xu, Jinying
Lv, Shuang
Hou, Yi
Xu, Kan
Sun, Dongjie
Zheng, Yangyang
Zhang, Zechuan
Li, Xianglan
Li, Yulin
Chi, Guangfan
author_sort Xu, Jinying
collection PubMed
description MicroRNAs (miRNAs) play an essential role in articular cartilage development and growth. However, the exact mechanisms involved in this process remain unknown. In the present study, we investigated the biological functions of miR-27b during hypertrophic differentiation of rat articular chondrocytes. Based on in situ hybridization and immunohistochemistry, we report that miR-27b expression is reduced in the hypertrophic zone of articular cartilage, but expression of peroxisome proliferator-activated receptor γ (Pparγ) is increased. Dual-luciferase reporter gene assay and Western blot analysis demonstrated that Pparγ2 is a target of miR-27b. Overexpression of miR-27b inhibited expression of Pparγ2, as well as type X collagen (Col10a1) and matrix metalloproteinase 13 (Mmp13), while significantly promoting the expression of Sex-determining Region-box 9 (Sox9) and type II collagen (Col2a1) at both the mRNA and protein levels. Rosiglitazone, a Pparγ agonist, suppressed Col2a1 expression, while promoting expression of runt-related transcription factor 2 (Runx2) and Col10a1 in a concentration-dependent manner. siRNA-mediated knockdown of Pparγ2 caused an increase in protein levels of Col2a1. The present study demonstrates that miR-27b regulates chondrocyte hypertrophy in part by targetting Pparγ2, and that miR-27b may have important therapeutic implications in cartilage diseases.
format Online
Article
Text
id pubmed-6435457
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-64354572019-04-12 miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation Xu, Jinying Lv, Shuang Hou, Yi Xu, Kan Sun, Dongjie Zheng, Yangyang Zhang, Zechuan Li, Xianglan Li, Yulin Chi, Guangfan Biosci Rep Research Articles MicroRNAs (miRNAs) play an essential role in articular cartilage development and growth. However, the exact mechanisms involved in this process remain unknown. In the present study, we investigated the biological functions of miR-27b during hypertrophic differentiation of rat articular chondrocytes. Based on in situ hybridization and immunohistochemistry, we report that miR-27b expression is reduced in the hypertrophic zone of articular cartilage, but expression of peroxisome proliferator-activated receptor γ (Pparγ) is increased. Dual-luciferase reporter gene assay and Western blot analysis demonstrated that Pparγ2 is a target of miR-27b. Overexpression of miR-27b inhibited expression of Pparγ2, as well as type X collagen (Col10a1) and matrix metalloproteinase 13 (Mmp13), while significantly promoting the expression of Sex-determining Region-box 9 (Sox9) and type II collagen (Col2a1) at both the mRNA and protein levels. Rosiglitazone, a Pparγ agonist, suppressed Col2a1 expression, while promoting expression of runt-related transcription factor 2 (Runx2) and Col10a1 in a concentration-dependent manner. siRNA-mediated knockdown of Pparγ2 caused an increase in protein levels of Col2a1. The present study demonstrates that miR-27b regulates chondrocyte hypertrophy in part by targetting Pparγ2, and that miR-27b may have important therapeutic implications in cartilage diseases. Portland Press Ltd. 2018-01-10 /pmc/articles/PMC6435457/ /pubmed/29187585 http://dx.doi.org/10.1042/BSR20171109 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Xu, Jinying
Lv, Shuang
Hou, Yi
Xu, Kan
Sun, Dongjie
Zheng, Yangyang
Zhang, Zechuan
Li, Xianglan
Li, Yulin
Chi, Guangfan
miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
title miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
title_full miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
title_fullStr miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
title_full_unstemmed miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
title_short miR-27b promotes type II collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
title_sort mir-27b promotes type ii collagen expression by targetting peroxisome proliferator-activated receptor-γ2 during rat articular chondrocyte differentiation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435457/
https://www.ncbi.nlm.nih.gov/pubmed/29187585
http://dx.doi.org/10.1042/BSR20171109
work_keys_str_mv AT xujinying mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT lvshuang mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT houyi mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT xukan mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT sundongjie mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT zhengyangyang mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT zhangzechuan mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT lixianglan mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT liyulin mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation
AT chiguangfan mir27bpromotestypeiicollagenexpressionbytargettingperoxisomeproliferatoractivatedreceptorg2duringratarticularchondrocytedifferentiation