Cargando…

Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli

Fluoroquinolones are important therapeutics in human and veterinary medicine. This study aimed to retrospectively analyse sedimentation dusts from intensive-livestock-farming barns for fluoroquinolones and investigate the association between resistant Escherichia coli and the detected drugs. Sedimen...

Descripción completa

Detalles Bibliográficos
Autores principales: Schulz, Jochen, Kemper, Nicole, Hartung, Joerg, Janusch, Franziska, Mohring, Siegrun A. I., Hamscher, Gerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435704/
https://www.ncbi.nlm.nih.gov/pubmed/30914675
http://dx.doi.org/10.1038/s41598-019-41528-z
_version_ 1783406691924049920
author Schulz, Jochen
Kemper, Nicole
Hartung, Joerg
Janusch, Franziska
Mohring, Siegrun A. I.
Hamscher, Gerd
author_facet Schulz, Jochen
Kemper, Nicole
Hartung, Joerg
Janusch, Franziska
Mohring, Siegrun A. I.
Hamscher, Gerd
author_sort Schulz, Jochen
collection PubMed
description Fluoroquinolones are important therapeutics in human and veterinary medicine. This study aimed to retrospectively analyse sedimentation dusts from intensive-livestock-farming barns for fluoroquinolones and investigate the association between resistant Escherichia coli and the detected drugs. Sedimentation-dust samples (n = 125) collected (1980–2009) at 14 barns of unknown-treatment status were analysed by HPLC and tandem-mass spectroscopy to detect enrofloxacin, ciprofloxacin, marbofloxacin, and difloxacin. Recent microbiological data were included to investigate the relationship between fluoroquinolone presence and fluoroquinolone-resistant E. coli. Fifty-nine dust samples (47%) from seven barns contained fluoroquinolone residues. Up to three different fluoroquinolones were detected in pig and broiler barns. Fluoroquinolone concentrations ranged from 10-pg/mg to 46-ng/mg dust. Fluoroquinolone-resistant E. coli were isolated from four barns. Of all the dust samples, 22% contained non-susceptible isolates. Non-susceptible isolate presence in the dust was significantly associated (p = 0.0283) with detecting the drugs, while drug detection increased the odds (4-fold) of finding non-susceptible E. coli (odds ratio = 3.9877, 95% CI: 1.2854–12.3712). This retrospective study shows that fluoroquinolone usage leads to dust contamination. We conclude that farmers and animals inhale/swallow fluoroquinolones and fluoroquinolone-resistant bacteria due to drug application. Furthermore, uncontrolled drug emissions via air exhausted from the barns can be assumed.
format Online
Article
Text
id pubmed-6435704
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-64357042019-04-03 Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli Schulz, Jochen Kemper, Nicole Hartung, Joerg Janusch, Franziska Mohring, Siegrun A. I. Hamscher, Gerd Sci Rep Article Fluoroquinolones are important therapeutics in human and veterinary medicine. This study aimed to retrospectively analyse sedimentation dusts from intensive-livestock-farming barns for fluoroquinolones and investigate the association between resistant Escherichia coli and the detected drugs. Sedimentation-dust samples (n = 125) collected (1980–2009) at 14 barns of unknown-treatment status were analysed by HPLC and tandem-mass spectroscopy to detect enrofloxacin, ciprofloxacin, marbofloxacin, and difloxacin. Recent microbiological data were included to investigate the relationship between fluoroquinolone presence and fluoroquinolone-resistant E. coli. Fifty-nine dust samples (47%) from seven barns contained fluoroquinolone residues. Up to three different fluoroquinolones were detected in pig and broiler barns. Fluoroquinolone concentrations ranged from 10-pg/mg to 46-ng/mg dust. Fluoroquinolone-resistant E. coli were isolated from four barns. Of all the dust samples, 22% contained non-susceptible isolates. Non-susceptible isolate presence in the dust was significantly associated (p = 0.0283) with detecting the drugs, while drug detection increased the odds (4-fold) of finding non-susceptible E. coli (odds ratio = 3.9877, 95% CI: 1.2854–12.3712). This retrospective study shows that fluoroquinolone usage leads to dust contamination. We conclude that farmers and animals inhale/swallow fluoroquinolones and fluoroquinolone-resistant bacteria due to drug application. Furthermore, uncontrolled drug emissions via air exhausted from the barns can be assumed. Nature Publishing Group UK 2019-03-26 /pmc/articles/PMC6435704/ /pubmed/30914675 http://dx.doi.org/10.1038/s41598-019-41528-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schulz, Jochen
Kemper, Nicole
Hartung, Joerg
Janusch, Franziska
Mohring, Siegrun A. I.
Hamscher, Gerd
Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli
title Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli
title_full Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli
title_fullStr Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli
title_full_unstemmed Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli
title_short Analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant Escherichia coli
title_sort analysis of fluoroquinolones in dusts from intensive livestock farming and the co-occurrence of fluoroquinolone-resistant escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435704/
https://www.ncbi.nlm.nih.gov/pubmed/30914675
http://dx.doi.org/10.1038/s41598-019-41528-z
work_keys_str_mv AT schulzjochen analysisoffluoroquinolonesindustsfromintensivelivestockfarmingandthecooccurrenceoffluoroquinoloneresistantescherichiacoli
AT kempernicole analysisoffluoroquinolonesindustsfromintensivelivestockfarmingandthecooccurrenceoffluoroquinoloneresistantescherichiacoli
AT hartungjoerg analysisoffluoroquinolonesindustsfromintensivelivestockfarmingandthecooccurrenceoffluoroquinoloneresistantescherichiacoli
AT januschfranziska analysisoffluoroquinolonesindustsfromintensivelivestockfarmingandthecooccurrenceoffluoroquinoloneresistantescherichiacoli
AT mohringsiegrunai analysisoffluoroquinolonesindustsfromintensivelivestockfarmingandthecooccurrenceoffluoroquinoloneresistantescherichiacoli
AT hamschergerd analysisoffluoroquinolonesindustsfromintensivelivestockfarmingandthecooccurrenceoffluoroquinoloneresistantescherichiacoli