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Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment

The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation. Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo. It would be...

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Autores principales: Zhou, Xianyu, Ma, Yan, Liu, Fei, Gu, Chuan, Wang, Xiuxia, Xia, Huitang, Zhou, Guangdong, Huang, Jinny, Luo, Xusong, Yang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435804/
https://www.ncbi.nlm.nih.gov/pubmed/30914712
http://dx.doi.org/10.1038/s41598-019-41611-5
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author Zhou, Xianyu
Ma, Yan
Liu, Fei
Gu, Chuan
Wang, Xiuxia
Xia, Huitang
Zhou, Guangdong
Huang, Jinny
Luo, Xusong
Yang, Jun
author_facet Zhou, Xianyu
Ma, Yan
Liu, Fei
Gu, Chuan
Wang, Xiuxia
Xia, Huitang
Zhou, Guangdong
Huang, Jinny
Luo, Xusong
Yang, Jun
author_sort Zhou, Xianyu
collection PubMed
description The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation. Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo. It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF). One potential method for MelSCs isolation is the “side population” technique, which is frequently used to isolate hematopoietic and tumor stem cells. In the present study, we decided to isolate HuHF MelSCs using “side population” to investigate their melanotic function. By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes. Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation. In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation.
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spelling pubmed-64358042019-04-03 Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment Zhou, Xianyu Ma, Yan Liu, Fei Gu, Chuan Wang, Xiuxia Xia, Huitang Zhou, Guangdong Huang, Jinny Luo, Xusong Yang, Jun Sci Rep Article The hair follicle serves as a melanocyte reservoir for both hair and skin pigmentation. Melanocyte stem cells (MelSCs) and melanocyte progenitors reside in the bulge/sub-bulge region of the lower permanent portion of the hair follicle and play a vital role for repigmentation in vitiligo. It would be beneficial to isolate MelSCs in order to further study their function in pigmentary disorders; however, due to the lack of specific molecular surface markers, this has not yet been successfully accomplished in human hair follicles (HuHF). One potential method for MelSCs isolation is the “side population” technique, which is frequently used to isolate hematopoietic and tumor stem cells. In the present study, we decided to isolate HuHF MelSCs using “side population” to investigate their melanotic function. By analyzing mRNA expression of TYR, SOX10, and MITF, melanosome structure, and immunofluorescence with melanocyte-specific markers, we revealed that the SP-fraction contained MelSCs with an admixture of differentiated melanocytes. Furthermore, our in vivo studies indicated that differentiated SP-fraction cells, when fabricated into a cell-chitosan/gelatin composite, could transiently repopulate immunologically compromised mice skin to regain pigmentation. In summary, the SP technique is capable of isolating HuHF MelSCs that can potentially be used to repopulate skin for pigmentation. Nature Publishing Group UK 2019-03-26 /pmc/articles/PMC6435804/ /pubmed/30914712 http://dx.doi.org/10.1038/s41598-019-41611-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zhou, Xianyu
Ma, Yan
Liu, Fei
Gu, Chuan
Wang, Xiuxia
Xia, Huitang
Zhou, Guangdong
Huang, Jinny
Luo, Xusong
Yang, Jun
Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
title Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
title_full Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
title_fullStr Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
title_full_unstemmed Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
title_short Melanocyte Chitosan/Gelatin Composite Fabrication with Human Outer Root Sheath-Derived Cells to Produce Pigment
title_sort melanocyte chitosan/gelatin composite fabrication with human outer root sheath-derived cells to produce pigment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435804/
https://www.ncbi.nlm.nih.gov/pubmed/30914712
http://dx.doi.org/10.1038/s41598-019-41611-5
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