Cargando…

Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa

In this study, we investigated the diversity of drug‐resistant Mycobacterium tuberculosis isolates from families who own cattle in the Eastern Cape Province of South Africa using spoligotyping and mycobacterial interspersed repetitive‐unit‐variable number tandem repeat (MIRU‐VNTR) typing. The Mycoba...

Descripción completa

Detalles Bibliográficos
Autores principales: Bhembe, Nolwazi L., Nwodo, Uchechukwu U., Okoh, Anthony I., Obi, Chikwelu L., Mabinya, Leonard V., Green, Ezekiel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436438/
https://www.ncbi.nlm.nih.gov/pubmed/30801981
http://dx.doi.org/10.1002/mbo3.449
_version_ 1783406814898946048
author Bhembe, Nolwazi L.
Nwodo, Uchechukwu U.
Okoh, Anthony I.
Obi, Chikwelu L.
Mabinya, Leonard V.
Green, Ezekiel
author_facet Bhembe, Nolwazi L.
Nwodo, Uchechukwu U.
Okoh, Anthony I.
Obi, Chikwelu L.
Mabinya, Leonard V.
Green, Ezekiel
author_sort Bhembe, Nolwazi L.
collection PubMed
description In this study, we investigated the diversity of drug‐resistant Mycobacterium tuberculosis isolates from families who own cattle in the Eastern Cape Province of South Africa using spoligotyping and mycobacterial interspersed repetitive‐unit‐variable number tandem repeat (MIRU‐VNTR) typing. The Mycobacterium tuberculosis was investigated using MIRU‐VNTR and the Mycobacterium tuberculosis families were evaluated using spoligotyping. Spoligotyping grouped 91% of the isolates into seven clusters, while 9% of the deoxyribonucleic acid (DNA) from TB isolates were unclustered from a total of 154 DNA used. Previously described shared types were observed in 89.6% of the isolates, with the Beijing family, SIT1, the principal genotype in the province, while the families T, SIT53 and X1, SIT1329 were the least detected genotypes. MIRU‐VNTR grouped 81% of the isolates in 23 clusters while 19% were unclustered. A combination of the VNTR and spoligotyping grouped 79% of the isolates into 23 clusters with 21% unclustered. The low level of diversity and the clonal spread of drug‐resistant Mycobacterium tuberculosis isolates advocate that the spread of TB in this study may be instigated by the clonal spread of Beijing genotype. The results from this study provide vital information about the lack of TB control and distribution of Mycobacterium tuberculosis complex strain types in the Eastern Cape Province of South Africa.
format Online
Article
Text
id pubmed-6436438
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-64364382019-04-08 Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa Bhembe, Nolwazi L. Nwodo, Uchechukwu U. Okoh, Anthony I. Obi, Chikwelu L. Mabinya, Leonard V. Green, Ezekiel Microbiologyopen Original Articles In this study, we investigated the diversity of drug‐resistant Mycobacterium tuberculosis isolates from families who own cattle in the Eastern Cape Province of South Africa using spoligotyping and mycobacterial interspersed repetitive‐unit‐variable number tandem repeat (MIRU‐VNTR) typing. The Mycobacterium tuberculosis was investigated using MIRU‐VNTR and the Mycobacterium tuberculosis families were evaluated using spoligotyping. Spoligotyping grouped 91% of the isolates into seven clusters, while 9% of the deoxyribonucleic acid (DNA) from TB isolates were unclustered from a total of 154 DNA used. Previously described shared types were observed in 89.6% of the isolates, with the Beijing family, SIT1, the principal genotype in the province, while the families T, SIT53 and X1, SIT1329 were the least detected genotypes. MIRU‐VNTR grouped 81% of the isolates in 23 clusters while 19% were unclustered. A combination of the VNTR and spoligotyping grouped 79% of the isolates into 23 clusters with 21% unclustered. The low level of diversity and the clonal spread of drug‐resistant Mycobacterium tuberculosis isolates advocate that the spread of TB in this study may be instigated by the clonal spread of Beijing genotype. The results from this study provide vital information about the lack of TB control and distribution of Mycobacterium tuberculosis complex strain types in the Eastern Cape Province of South Africa. John Wiley and Sons Inc. 2019-02-23 /pmc/articles/PMC6436438/ /pubmed/30801981 http://dx.doi.org/10.1002/mbo3.449 Text en © 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Bhembe, Nolwazi L.
Nwodo, Uchechukwu U.
Okoh, Anthony I.
Obi, Chikwelu L.
Mabinya, Leonard V.
Green, Ezekiel
Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa
title Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa
title_full Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa
title_fullStr Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa
title_full_unstemmed Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa
title_short Clonality and genetic profiles of drug‐resistant Mycobacterium tuberculosis in the Eastern Cape Province, South Africa
title_sort clonality and genetic profiles of drug‐resistant mycobacterium tuberculosis in the eastern cape province, south africa
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436438/
https://www.ncbi.nlm.nih.gov/pubmed/30801981
http://dx.doi.org/10.1002/mbo3.449
work_keys_str_mv AT bhembenolwazil clonalityandgeneticprofilesofdrugresistantmycobacteriumtuberculosisintheeasterncapeprovincesouthafrica
AT nwodouchechukwuu clonalityandgeneticprofilesofdrugresistantmycobacteriumtuberculosisintheeasterncapeprovincesouthafrica
AT okohanthonyi clonalityandgeneticprofilesofdrugresistantmycobacteriumtuberculosisintheeasterncapeprovincesouthafrica
AT obichikwelul clonalityandgeneticprofilesofdrugresistantmycobacteriumtuberculosisintheeasterncapeprovincesouthafrica
AT mabinyaleonardv clonalityandgeneticprofilesofdrugresistantmycobacteriumtuberculosisintheeasterncapeprovincesouthafrica
AT greenezekiel clonalityandgeneticprofilesofdrugresistantmycobacteriumtuberculosisintheeasterncapeprovincesouthafrica