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Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China
The cotton‐melon aphid, Aphis gossypii Glover, is a worldwide‐spreading species, and pesticide‐resistant populations are increasing rapidly. In this study, investigations were performed based on Illumina HiSeq sequencing of the 16S rDNA V4 region for the bacterial communities embodied as intracellul...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436440/ https://www.ncbi.nlm.nih.gov/pubmed/29877631 http://dx.doi.org/10.1002/mbo3.652 |
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author | Zhang, Shuai Luo, Junyu Wang, Li Zhang, Lijuan Zhu, Xiangzhen Jiang, Weili Cui, Jinjie |
author_facet | Zhang, Shuai Luo, Junyu Wang, Li Zhang, Lijuan Zhu, Xiangzhen Jiang, Weili Cui, Jinjie |
author_sort | Zhang, Shuai |
collection | PubMed |
description | The cotton‐melon aphid, Aphis gossypii Glover, is a worldwide‐spreading species, and pesticide‐resistant populations are increasing rapidly. In this study, investigations were performed based on Illumina HiSeq sequencing of the 16S rDNA V4 region for the bacterial communities embodied as intracellular symbionts under natural and in pesticide‐treated populations of A. gossypii. The results revealed that more than 82% of bacterial communities belonged to the phylum Proteobacteria in which the maximum proportion (53.24%) was of the genus Arsenophonus; Hamiltonella composed 22.31; and 1.37% was of the genus Acinetobacter. The relative abundance of Hamiltonella was obvious, vertically transmitted, divided into two groups, and its infection influenced the bacterial communities in A. gossypii. Symbiont density and composition were changed in samples tested on different days. Azadirachtin and phoxim influenced on the composition of bacterial communities. Different biomarkers were used for pesticide‐treated samples with LEfSe results. These findings will increase awareness regarding bacterial communities in naturally occurring populations of A. gossypii and pave the way to study the relationship between symbionts and pesticide resistance. |
format | Online Article Text |
id | pubmed-6436440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64364402019-04-08 Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China Zhang, Shuai Luo, Junyu Wang, Li Zhang, Lijuan Zhu, Xiangzhen Jiang, Weili Cui, Jinjie Microbiologyopen Original Articles The cotton‐melon aphid, Aphis gossypii Glover, is a worldwide‐spreading species, and pesticide‐resistant populations are increasing rapidly. In this study, investigations were performed based on Illumina HiSeq sequencing of the 16S rDNA V4 region for the bacterial communities embodied as intracellular symbionts under natural and in pesticide‐treated populations of A. gossypii. The results revealed that more than 82% of bacterial communities belonged to the phylum Proteobacteria in which the maximum proportion (53.24%) was of the genus Arsenophonus; Hamiltonella composed 22.31; and 1.37% was of the genus Acinetobacter. The relative abundance of Hamiltonella was obvious, vertically transmitted, divided into two groups, and its infection influenced the bacterial communities in A. gossypii. Symbiont density and composition were changed in samples tested on different days. Azadirachtin and phoxim influenced on the composition of bacterial communities. Different biomarkers were used for pesticide‐treated samples with LEfSe results. These findings will increase awareness regarding bacterial communities in naturally occurring populations of A. gossypii and pave the way to study the relationship between symbionts and pesticide resistance. John Wiley and Sons Inc. 2018-06-07 /pmc/articles/PMC6436440/ /pubmed/29877631 http://dx.doi.org/10.1002/mbo3.652 Text en © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zhang, Shuai Luo, Junyu Wang, Li Zhang, Lijuan Zhu, Xiangzhen Jiang, Weili Cui, Jinjie Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China |
title | Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China |
title_full | Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China |
title_fullStr | Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China |
title_full_unstemmed | Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China |
title_short | Bacterial communities in natural versus pesticide‐treated Aphis gossypii populations in North China |
title_sort | bacterial communities in natural versus pesticide‐treated aphis gossypii populations in north china |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436440/ https://www.ncbi.nlm.nih.gov/pubmed/29877631 http://dx.doi.org/10.1002/mbo3.652 |
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