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Stress-Induced MazF-Mediated Proteins in Escherichia coli
Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437054/ https://www.ncbi.nlm.nih.gov/pubmed/30914510 http://dx.doi.org/10.1128/mBio.00340-19 |
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author | Nigam, Akanksha Ziv, Tamar Oron-Gottesman, Adi Engelberg-Kulka, Hanna |
author_facet | Nigam, Akanksha Ziv, Tamar Oron-Gottesman, Adi Engelberg-Kulka, Hanna |
author_sort | Nigam, Akanksha |
collection | PubMed |
description | Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through the chromosomally borne mazF gene. We show that the mRNAs of almost all of them are characterized by the presence of an ACA site up to 100 nucleotides upstream of the AUG initiator. Therefore, under stressful conditions, induced MazF processes mRNAs that are translated by STM. Furthermore, the presence of the ACA sites far upstream (up to 100 nucleotides) of the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual-translation mechanisms enable the bacterium under stress also to prepare proteins for immediate functions while coming back to normal growth conditions. |
format | Online Article Text |
id | pubmed-6437054 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-64370542019-04-03 Stress-Induced MazF-Mediated Proteins in Escherichia coli Nigam, Akanksha Ziv, Tamar Oron-Gottesman, Adi Engelberg-Kulka, Hanna mBio Research Article Escherichia coli mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. By that means, under stress, the induced MazF generates a stress-induced translation machinery (STM) composed of MazF-processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here, we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through the chromosomally borne mazF gene. We show that the mRNAs of almost all of them are characterized by the presence of an ACA site up to 100 nucleotides upstream of the AUG initiator. Therefore, under stressful conditions, induced MazF processes mRNAs that are translated by STM. Furthermore, the presence of the ACA sites far upstream (up to 100 nucleotides) of the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual-translation mechanisms enable the bacterium under stress also to prepare proteins for immediate functions while coming back to normal growth conditions. American Society for Microbiology 2019-03-26 /pmc/articles/PMC6437054/ /pubmed/30914510 http://dx.doi.org/10.1128/mBio.00340-19 Text en Copyright © 2019 Nigam et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Nigam, Akanksha Ziv, Tamar Oron-Gottesman, Adi Engelberg-Kulka, Hanna Stress-Induced MazF-Mediated Proteins in Escherichia coli |
title | Stress-Induced MazF-Mediated Proteins in Escherichia coli |
title_full | Stress-Induced MazF-Mediated Proteins in Escherichia coli |
title_fullStr | Stress-Induced MazF-Mediated Proteins in Escherichia coli |
title_full_unstemmed | Stress-Induced MazF-Mediated Proteins in Escherichia coli |
title_short | Stress-Induced MazF-Mediated Proteins in Escherichia coli |
title_sort | stress-induced mazf-mediated proteins in escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437054/ https://www.ncbi.nlm.nih.gov/pubmed/30914510 http://dx.doi.org/10.1128/mBio.00340-19 |
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