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Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
OBJECTIVE(S): Histone deacetylase inhibitory and cytotoxic activities of 18 naturally occuring terpenoids (ferutinin, stylosin, tschimgine and guaiol), coumarins (umbelliprenin, farnesiferone B, conferone, feselol, ligupersin A, conferdione, conferoside) and sulfur-containing derivatives (latisulfie...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437461/ https://www.ncbi.nlm.nih.gov/pubmed/30944714 http://dx.doi.org/10.22038/IJBMS.2018.34338.8151 |
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author | Soltani, Saba Amin, Gholamreza Salehi-Sourmaghi, Mohammad Hossein Iranshahi, Mehrdad |
author_facet | Soltani, Saba Amin, Gholamreza Salehi-Sourmaghi, Mohammad Hossein Iranshahi, Mehrdad |
author_sort | Soltani, Saba |
collection | PubMed |
description | OBJECTIVE(S): Histone deacetylase inhibitory and cytotoxic activities of 18 naturally occuring terpenoids (ferutinin, stylosin, tschimgine and guaiol), coumarins (umbelliprenin, farnesiferone B, conferone, feselol, ligupersin A, conferdione, conferoside) and sulfur-containing derivatives (latisulfies A-E, persicasulphides A and C) from the roots of three species of Ferula (Ferula latisecta, Ferula ovina and Ferula flabelliloba) were evaluated. MATERIALS AND METHODS: The cytotoxic activity of compounds was evaluated against human cancer cell lines (HeLa, HCT116, A2780 and A549) by AlamarBlue® assay using vorinostat as the positive control. On the other hand, we aimed to evaluate their inhibitory activities against pan-HDAC. RESULTS: The methanolic extract of the roots of F. flabelliloba was subjected to silica gel column chromatography. Further purification by preparative thin-layer chromatography (PTLC) and semipreparative RP-HPLC yielded twelve known compounds (1-12). This is the first report on the isolation of guaiol (1), persicasulphide C (3) and conferoside (10) from the roots of F. flabelliloba. Six compounds including persicasulfide A, conferone, feselol, latisufide C, conferoside and ferutinin showed cytotoxic activity with IC(50 )values in the range of 11.61-49.40 μM against cancer cells and pan-HDAC inhibitory activity with IC(50) values in the range of 1.06-35.27 μM. CONCLUSION: Results indicated that persicasulfide A (2), conferone (6) and feselol (7) showed moderate cytotoxicity with IC50 values in the range of 11.76-39.24 μM against cancer cells and potent pan-HDAC inhibitory activity with IC(50) values in the range of 1.06-10.73 μM. Conferone was more active than others with a higher potency for HDAC inhibition (1.06- 1.17 μM). |
format | Online Article Text |
id | pubmed-6437461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-64374612019-04-03 Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula Soltani, Saba Amin, Gholamreza Salehi-Sourmaghi, Mohammad Hossein Iranshahi, Mehrdad Iran J Basic Med Sci Original Article OBJECTIVE(S): Histone deacetylase inhibitory and cytotoxic activities of 18 naturally occuring terpenoids (ferutinin, stylosin, tschimgine and guaiol), coumarins (umbelliprenin, farnesiferone B, conferone, feselol, ligupersin A, conferdione, conferoside) and sulfur-containing derivatives (latisulfies A-E, persicasulphides A and C) from the roots of three species of Ferula (Ferula latisecta, Ferula ovina and Ferula flabelliloba) were evaluated. MATERIALS AND METHODS: The cytotoxic activity of compounds was evaluated against human cancer cell lines (HeLa, HCT116, A2780 and A549) by AlamarBlue® assay using vorinostat as the positive control. On the other hand, we aimed to evaluate their inhibitory activities against pan-HDAC. RESULTS: The methanolic extract of the roots of F. flabelliloba was subjected to silica gel column chromatography. Further purification by preparative thin-layer chromatography (PTLC) and semipreparative RP-HPLC yielded twelve known compounds (1-12). This is the first report on the isolation of guaiol (1), persicasulphide C (3) and conferoside (10) from the roots of F. flabelliloba. Six compounds including persicasulfide A, conferone, feselol, latisufide C, conferoside and ferutinin showed cytotoxic activity with IC(50 )values in the range of 11.61-49.40 μM against cancer cells and pan-HDAC inhibitory activity with IC(50) values in the range of 1.06-35.27 μM. CONCLUSION: Results indicated that persicasulfide A (2), conferone (6) and feselol (7) showed moderate cytotoxicity with IC50 values in the range of 11.76-39.24 μM against cancer cells and potent pan-HDAC inhibitory activity with IC(50) values in the range of 1.06-10.73 μM. Conferone was more active than others with a higher potency for HDAC inhibition (1.06- 1.17 μM). Mashhad University of Medical Sciences 2019-01 /pmc/articles/PMC6437461/ /pubmed/30944714 http://dx.doi.org/10.22038/IJBMS.2018.34338.8151 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Soltani, Saba Amin, Gholamreza Salehi-Sourmaghi, Mohammad Hossein Iranshahi, Mehrdad Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula |
title | Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
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title_full | Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
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title_fullStr | Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
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title_full_unstemmed | Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
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title_short | Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula
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title_sort | histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of ferula |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437461/ https://www.ncbi.nlm.nih.gov/pubmed/30944714 http://dx.doi.org/10.22038/IJBMS.2018.34338.8151 |
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