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Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish

BACKGROUND: Diabetic sensorineural damage is a complication of the sensory neural system, resulting from long-term hyperglycemia. Red ginseng (RG) has shown efficacy for treatment of various diseases, including diabetes mellitus; however, there is little research about its benefit for treating senso...

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Autores principales: Nam, Youn Hee, Moon, Hyo Won, Lee, Yeong Ro, Kim, Eun Young, Rodriguez, Isabel, Jeong, Seo Yule, Castañeda, Rodrigo, Park, Ji-Ho, Choung, Se-Young, Hong, Bin Na, Kang, Tong Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437664/
https://www.ncbi.nlm.nih.gov/pubmed/30976165
http://dx.doi.org/10.1016/j.jgr.2018.02.006
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author Nam, Youn Hee
Moon, Hyo Won
Lee, Yeong Ro
Kim, Eun Young
Rodriguez, Isabel
Jeong, Seo Yule
Castañeda, Rodrigo
Park, Ji-Ho
Choung, Se-Young
Hong, Bin Na
Kang, Tong Ho
author_facet Nam, Youn Hee
Moon, Hyo Won
Lee, Yeong Ro
Kim, Eun Young
Rodriguez, Isabel
Jeong, Seo Yule
Castañeda, Rodrigo
Park, Ji-Ho
Choung, Se-Young
Hong, Bin Na
Kang, Tong Ho
author_sort Nam, Youn Hee
collection PubMed
description BACKGROUND: Diabetic sensorineural damage is a complication of the sensory neural system, resulting from long-term hyperglycemia. Red ginseng (RG) has shown efficacy for treatment of various diseases, including diabetes mellitus; however, there is little research about its benefit for treating sensorineural damage. Therefore, we aim to evaluate RG efficacy in alloxan-induced diabetic neuromast (AIDN) zebrafish. METHODS: In this study, we developed and validated an AIDN zebrafish model. To assess RG effectiveness, we observed morphological changes in live neuromast zebrafish. Also, zebrafish has been observed to have an ultrastructure of hair-cell cilia under scanning electron microscopy. Thus, we recorded these physiological traits to assess hair cell function. Finally, we confirmed that RG promoted neuromast recovery via nerve growth factor signaling pathway markers. RESULTS: First, we established an AIDN zebrafish model. Using this model, we showed via live neuromast imaging that RG fostered recovery of sensorineural damage. Damaged hair cell cilia were recovered in AIDN zebrafish. Furthermore, RG rescued damaged hair cell function through cell membrane ion balance. CONCLUSION: Our data suggest that RG potentially facilitates recovery in AIDN zebrafish, and its mechanism seems to be promotion of the nerve growth factor pathway through increased expression of topomyosin receptor kinase A, transient receptor potential channel vanilloid subfamily type 1, and mitogen-activated protein kinase phosphorylation.
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spelling pubmed-64376642019-04-11 Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish Nam, Youn Hee Moon, Hyo Won Lee, Yeong Ro Kim, Eun Young Rodriguez, Isabel Jeong, Seo Yule Castañeda, Rodrigo Park, Ji-Ho Choung, Se-Young Hong, Bin Na Kang, Tong Ho J Ginseng Res Research Article BACKGROUND: Diabetic sensorineural damage is a complication of the sensory neural system, resulting from long-term hyperglycemia. Red ginseng (RG) has shown efficacy for treatment of various diseases, including diabetes mellitus; however, there is little research about its benefit for treating sensorineural damage. Therefore, we aim to evaluate RG efficacy in alloxan-induced diabetic neuromast (AIDN) zebrafish. METHODS: In this study, we developed and validated an AIDN zebrafish model. To assess RG effectiveness, we observed morphological changes in live neuromast zebrafish. Also, zebrafish has been observed to have an ultrastructure of hair-cell cilia under scanning electron microscopy. Thus, we recorded these physiological traits to assess hair cell function. Finally, we confirmed that RG promoted neuromast recovery via nerve growth factor signaling pathway markers. RESULTS: First, we established an AIDN zebrafish model. Using this model, we showed via live neuromast imaging that RG fostered recovery of sensorineural damage. Damaged hair cell cilia were recovered in AIDN zebrafish. Furthermore, RG rescued damaged hair cell function through cell membrane ion balance. CONCLUSION: Our data suggest that RG potentially facilitates recovery in AIDN zebrafish, and its mechanism seems to be promotion of the nerve growth factor pathway through increased expression of topomyosin receptor kinase A, transient receptor potential channel vanilloid subfamily type 1, and mitogen-activated protein kinase phosphorylation. Elsevier 2019-04 2018-02-16 /pmc/articles/PMC6437664/ /pubmed/30976165 http://dx.doi.org/10.1016/j.jgr.2018.02.006 Text en © 2018 The Korean Society of Ginseng, Published by Elsevier Korea LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Nam, Youn Hee
Moon, Hyo Won
Lee, Yeong Ro
Kim, Eun Young
Rodriguez, Isabel
Jeong, Seo Yule
Castañeda, Rodrigo
Park, Ji-Ho
Choung, Se-Young
Hong, Bin Na
Kang, Tong Ho
Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
title Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
title_full Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
title_fullStr Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
title_full_unstemmed Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
title_short Panax ginseng (Korea Red Ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
title_sort panax ginseng (korea red ginseng) repairs diabetic sensorineural damage through promotion of the nerve growth factor pathway in diabetic zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437664/
https://www.ncbi.nlm.nih.gov/pubmed/30976165
http://dx.doi.org/10.1016/j.jgr.2018.02.006
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