Cell transfection of purified cytolethal distending toxin B subunits allows comparing their nuclease activity while plasmid degradation assay does not

The Cytolethal Distending Toxin (CDT) is produced by many pathogenic bacteria. CDT is known to induce genomic DNA damage to host eukaryotic cells through its catalytic subunit, CdtB. CdtB is structurally homologous to DNase I and has a nuclease activity, dependent on several key residues. Yet some d...

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Detalles Bibliográficos
Autores principales: Pons, Benoît J., Bezine, Elisabeth, Hanique, Mélissa, Guillet, Valérie, Mourey, Lionel, Chicher, Johana, Frisan, Teresa, Vignard, Julien, Mirey, Gladys
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438463/
https://www.ncbi.nlm.nih.gov/pubmed/30921382
http://dx.doi.org/10.1371/journal.pone.0214313

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438463/
https://www.ncbi.nlm.nih.gov/pubmed/30921382
http://dx.doi.org/10.1371/journal.pone.0214313

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